7-deazaadenine and 7-deazapurine

7-deazaadenine has been researched along with 7-deazapurine* in 2 studies

Other Studies

2 other study(ies) available for 7-deazaadenine and 7-deazapurine

Article	Year
Brightly Fluorescent 2'-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry. Bioconjugate chemistry, 2018, 11-21, Volume: 29, Issue:11 Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dN Topics: Adenine; Boron Compounds; Cell Line, Tumor; Cytosine; Deoxyribonucleosides; DNA; Flow Cytometry; Fluorescent Dyes; Humans; Methylation; Optical Imaging; Polyphosphates; Purines; Staining and Labeling	2018
Silver Ions in Non-canonical DNA Base Pairs: Metal-Mediated Mismatch Stabilization of 2'-Deoxyadenosine and 7-Deazapurine Derivatives with 2'-Deoxycytidine and 2'-Deoxyguanosine. Chemistry (Weinheim an der Bergstrasse, Germany), 2016, Sep-05, Volume: 22, Issue:37 Novel silver-mediated dA-dC, dA-dC, and dA-dG base pairs were formed in a natural DNA double helix environment (dA* denotes 7-deaza-dA, 7-deaza-7-iodo-dA, and 7-cyclopropyl-7-deaza-dA). 7-Deazapurine nucleosides enforce silver ion binding and direct metal-mediated base pair formation to their Watson-Crick face. New phosphoramidites were prepared from 7-deaza-dA, 7-deaza-7-iodo-dA, and 7-cyclopropyl-7-deaza-dA, which contain labile isobutyryl protecting groups. Solid-phase synthesis furnished oligonucleotides that contain mismatches in near central positions. Increased thermal stabilities (higher Tm values) were observed for oligonucleotide duplexes with non-canonical dA*-dC and dA-dC pairs in the presence of silver ions. The stability of the silver-mediated base pairs was pH dependent. Silver ion binding was not observed for the dA-dG mismatch but took place when mismatches were formed between 7-deazaadenine and guanine. The specific binding of silver ions was confirmed by stoichiometric UV titration experiments, which proved that one silver ion is captured by one mismatch. The stability increase of canonical DNA mismatches might have an impact on cellular DNA repair. Topics: Adenine; Anions; Base Pair Mismatch; Base Pairing; Binding Sites; Deoxyadenosines; Deoxycytidine; Deoxyguanosine; DNA; Guanine; Nucleic Acid Conformation; Nucleosides; Oligonucleotides; Purines; Silver; Solid-Phase Synthesis Techniques	2016

Article

Year

Brightly Fluorescent 2'-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry.

Bioconjugate chemistry, 2018, 11-21, Volume: 29, Issue:11

Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dN

Topics: Adenine; Boron Compounds; Cell Line, Tumor; Cytosine; Deoxyribonucleosides; DNA; Flow Cytometry; Fluorescent Dyes; Humans; Methylation; Optical Imaging; Polyphosphates; Purines; Staining and Labeling

2018

Silver Ions in Non-canonical DNA Base Pairs: Metal-Mediated Mismatch Stabilization of 2'-Deoxyadenosine and 7-Deazapurine Derivatives with 2'-Deoxycytidine and 2'-Deoxyguanosine.

Chemistry (Weinheim an der Bergstrasse, Germany), 2016, Sep-05, Volume: 22, Issue:37

Novel silver-mediated dA-dC, dA*-dC, and dA*-dG base pairs were formed in a natural DNA double helix environment (dA* denotes 7-deaza-dA, 7-deaza-7-iodo-dA, and 7-cyclopropyl-7-deaza-dA). 7-Deazapurine nucleosides enforce silver ion binding and direct metal-mediated base pair formation to their Watson-Crick face. New phosphoramidites were prepared from 7-deaza-dA, 7-deaza-7-iodo-dA, and 7-cyclopropyl-7-deaza-dA, which contain labile isobutyryl protecting groups. Solid-phase synthesis furnished oligonucleotides that contain mismatches in near central positions. Increased thermal stabilities (higher Tm values) were observed for oligonucleotide duplexes with non-canonical dA*-dC and dA-dC pairs in the presence of silver ions. The stability of the silver-mediated base pairs was pH dependent. Silver ion binding was not observed for the dA-dG mismatch but took place when mismatches were formed between 7-deazaadenine and guanine. The specific binding of silver ions was confirmed by stoichiometric UV titration experiments, which proved that one silver ion is captured by one mismatch. The stability increase of canonical DNA mismatches might have an impact on cellular DNA repair.

Topics: Adenine; Anions; Base Pair Mismatch; Base Pairing; Binding Sites; Deoxyadenosines; Deoxycytidine; Deoxyguanosine; DNA; Guanine; Nucleic Acid Conformation; Nucleosides; Oligonucleotides; Purines; Silver; Solid-Phase Synthesis Techniques

2016