6-thioguanylic-acid has been researched along with 6-thioguanosine* in 2 studies
2 other study(ies) available for 6-thioguanylic-acid and 6-thioguanosine
| Article | Year |
|---|---|
Analysis of thiopurines using aqueous normal phase chromatography.
The chromatography of several thiopurines is investigated using aqueous normal phase (ANP) conditions in conjunction with a silica hydride-based column. Both isocratic and gradient elution modes are tested. Detection of higher concentration samples is done by UV to demonstrate feasibility in this format while lower concentration samples utilize mass spectrometry (MS). Repeatability of successive runs is also tested with particular attention to gradient methods where the equilibration time of the stationary phase can be evaluated. Topics: Chromatography, High Pressure Liquid; Guanine Nucleotides; Guanosine; Thioguanine; Thionucleosides; Thionucleotides | 2014 |
Conversion of 6-mercaptopurine to 6-thioguanylic acid in L-1210 cells and human leukemia cells.
The metabolism of 6-mercaptopurine (6-MP) in L-1210 mouse leukemia cells and human chronic myelocytic leukemia cells (CML cells) was examined. The acid-soluble fractions obtained from cells incubated with [8-14C]6-MP were chromatographed on a Dowex-1 formate resin column using a formic acid linear gradient elution system. Chromatography of the extract of L-1210 cells revealed four principal radioactive peaks. The fraction containing the third peak was hydrolyzed by snake venom 5'-nucleotidase (Crotalus adamanteus). Cellulose thin layer chromatography revealed that the radioactive peak of the hydrolysate corresponded to 6-thioguanosine. The results showed that 6-MP was converted to 6-thioinosinic acid (6-TIMP) and 6-thioguanylic acid (6-TGMP) in L-1210 cells. In order to elucidate the pathway of 6-MP conversion to 6-TGMP, we examined the interaction of [8-14C]6-TIMP and purified IMP dehydrogenase. It was found by DEAE-cellulose thin layer chromatography that the IMP dehydrogenase converted 6-TIMP to 6-thioxanthylic acid (6-TXMP). Dowex-1 chromatography of the acid-soluble extract of human CML cells incubated with [8-14C]-6-MP also revealed a radioactive peak corresponding to 6-TGMP. These results suggest that 6-MP is metabolized to 6-TGMP by serial conversion to 6-TIMP and 6-TXMP through the de novo GMP synthetic pathway in L-1210 cells and human CML cells. Topics: Animals; Cells, Cultured; Chromatography; Chromatography, DEAE-Cellulose; Guanine Nucleotides; Guanosine; Humans; IMP Dehydrogenase; Kinetics; Leukemia L1210; Leukemia, Myeloid; Mercaptopurine; Mice; Thionucleosides; Thionucleotides | 1985 |