6-mercapto-1-hexanol and 4-thiopyridine

Au nanoparticles modified with 4-mercaptopyridine and 6-mercapto-1-hexanol were used as coordination agents to prepare a novel hybrid nanomaterial with Ag:4,4'-bipyridine nanobelts. This nanohybrid was employed to modify glassy carbon electrodes and to construct a horseradish peroxidase-based mediatorless amperometric biosensor for H2O2. The electrode, poised at -100mV, exhibited a rapid response within 4s and a linear calibration range from 90pM to 6.5nM H2O2. The biosensor showed a high sensitivity of 283A/Mcm(2) and a very low detection limit of 45pM at a signal-to-noise ratio of 3. The enzyme biosensor showed high stability when stored at 4°C under dry conditions, retaining over 96% and 78% of its initial activity after 15 and 30days of storage at 4°C, respectively.

Topics: Biosensing Techniques; Calibration; Carbon; Electrochemical Techniques; Electrodes; Enzymes, Immobilized; Gold; Hexanols; Horseradish Peroxidase; Hydrogen Peroxide; Limit of Detection; Metal Nanoparticles; Pyridines; Reproducibility of Results; Signal-To-Noise Ratio; Sulfhydryl Compounds