6-ketoprostaglandin-f1-alpha and thiobarbituric-acid

The stable metabolite of prostacyclin 6-keto PGF1 alpha originating from the (1-14C) arachidonic acid, and lipid peroxidation expressed as thiobarbituric acid-reacting substance (TBARS) were studied in the aorta of rats fed a diet enriched in 2% w/w cholesterol autoxidation products for 24 hours prior to sacrifice. A slight increase was found in the amount of TBARS as well as in the conversion of (1-14C) arachidonic acid to 6-keto PGF1 alpha. These results suggest that in aorta there exists a protection mechanism which acts to increase the production of prostacyclin.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Aorta; Arachidonic Acid; Arachidonic Acids; Arteries; Cholesterol; Diet, Atherogenic; Epoprostenol; Lipid Peroxides; Male; Oxidation-Reduction; Rats; Rats, Inbred Strains; Thiobarbiturates