6-ketoprostaglandin-f1-alpha and protocatechuic-acid

The compounds of Radix Paeoniae Rubra (RPR) were isolated and identified by bioassay-guided method, and antithrombotic effects and mechanism were investigated by the acute blood stasis rat model. The RPR extract was evaluated by APTT, TT, PT, and FIB assays in vitro. Results indicated that RPR extract exhibited the anticoagulant activity. In order to find active compounds, six compounds were isolated and identified, and four compounds, paeoniflorin (Pae), pentagalloylglucose (Pen), albiflorin (Ali), and protocatechuic acid (Pro), exhibited the anticoagulant activity in vitro. Therefore, the antithrombosis effects of RPR extract and four active compounds were investigated in vivo by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of TXB

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Anticoagulants; Biological Assay; Bridged-Ring Compounds; Drugs, Chinese Herbal; Endothelin-1; Female; Fibrinolytic Agents; Glucosides; Hydrolyzable Tannins; Hydroxybenzoates; Male; Monoterpenes; Nitric Oxide Synthase Type III; Paeonia; Phytotherapy; Rats; Rats, Sprague-Dawley; Thrombosis; Thromboxane B2; Viscosity