6-beta-hydroxycortisol and boric-acid

6-beta-hydroxycortisol has been researched along with boric-acid* in 1 studies

Other Studies

1 other study(ies) available for 6-beta-hydroxycortisol and boric-acid

Article	Year
Automated on-line SPE LC-MS/MS method to quantitate 6beta-hydroxycortisol and cortisol in human urine: use of the 6beta-hydroxycortisol to cortisol ratio as an indicator of CYP3A4 activity. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005, Jul-25, Volume: 821, Issue:2 A sensitive method for quantitation of urinary 6beta-hydroxycortisol (6beta-HC) and cortisol using on-line SPE and LC-MS/MS was developed and validated. Human urine samples were injected directly onto an on-line solid phase extraction apparatus, Prospekt-2, followed by HPLC separation and electrospray triple quadrupole LC-MS/MS detection. The inter-day precision for the 6beta-HC:cortisol ratio was 7-9%. The lower limit of quantitation was 1 and 0.2 ng/mL for 6beta-HC and cortisol, respectively. Using the method we observed a diurnal variation on the 6beta-HC:cortisol ratio in healthy volunteers with the maximal ratio observed in the 2-10 pm urine collection period. Topics: Autoanalysis; Boric Acids; Chromatography, Liquid; Circadian Rhythm; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; Drug Stability; Female; Humans; Hydrocortisone; Male; Mass Spectrometry; Online Systems; Reference Values; Reproducibility of Results; Sensitivity and Specificity	2005

Article

Year

Automated on-line SPE LC-MS/MS method to quantitate 6beta-hydroxycortisol and cortisol in human urine: use of the 6beta-hydroxycortisol to cortisol ratio as an indicator of CYP3A4 activity.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005, Jul-25, Volume: 821, Issue:2

A sensitive method for quantitation of urinary 6beta-hydroxycortisol (6beta-HC) and cortisol using on-line SPE and LC-MS/MS was developed and validated. Human urine samples were injected directly onto an on-line solid phase extraction apparatus, Prospekt-2, followed by HPLC separation and electrospray triple quadrupole LC-MS/MS detection. The inter-day precision for the 6beta-HC:cortisol ratio was 7-9%. The lower limit of quantitation was 1 and 0.2 ng/mL for 6beta-HC and cortisol, respectively. Using the method we observed a diurnal variation on the 6beta-HC:cortisol ratio in healthy volunteers with the maximal ratio observed in the 2-10 pm urine collection period.

Topics: Autoanalysis; Boric Acids; Chromatography, Liquid; Circadian Rhythm; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; Drug Stability; Female; Humans; Hydrocortisone; Male; Mass Spectrometry; Online Systems; Reference Values; Reproducibility of Results; Sensitivity and Specificity

2005