6-7-dihydroxyflavone and 4-(4-6-dimethoxy-1-3-5-triazin-2-yl)-4-methylmorpholinium-chloride

We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.

Topics: Animals; Antineoplastic Agents; Cell Proliferation; Cell Survival; Drug Screening Assays, Antitumor; Flavones; HeLa Cells; Hep G2 Cells; Humans; Hyaluronic Acid; Mice; Molecular Structure; Morpholines; Nanogels; NIH 3T3 Cells; Particle Size; Polyethylene Glycols; Polyethyleneimine; Surface Properties