Page last updated: 2024-08-24

5-methylcytosine and sulfites

5-methylcytosine has been researched along with sulfites in 124 studies

Research

Studies (124)

TimeframeStudies, this research(%)All Research%
pre-19902 (1.61)18.7374
1990's19 (15.32)18.2507
2000's18 (14.52)29.6817
2010's65 (52.42)24.3611
2020's20 (16.13)2.80

Authors

AuthorsStudies
Hayatsu, H; Shiragami, M1
Collis, CM; Frommer, M; Grigg, GW; McDonald, LE; Millar, DS; Molloy, PL; Paul, CL; Watt, F1
Deutsch, WA; Green, DA1
Clark, SJ; Frommer, M; Harrison, J1
Jost, JP; Raizis, AM; Schmitt, F1
Clark, SJ; Frommer, M; Harrison, J; Paul, CL1
Clark, S; Grigg, G1
Bird, AP; Charlton, J; Feil, R; Reik, W; Walter, J1
Prydz, H; Reeben, M1
Heinlein, M; Kunze, R; Wang, L1
Grigg, GW1
DePamphilis, ML; Rein, T; Zorbas, H1
Buiting, K; Dittrich, B; Doerfler, W; Horsthemke, B; Schmitz, B; Zeschnigk, M1
Magill, C; Magill, JM; Newton, RJ; Zhou, Y1
Clark, SJ; Melki, JR; Millar, DS; Paul, CL; Stirzaker, C; Warnecke, PM1
Davey, MW; Grigg, GW; Paulin, R; Piper, AA1
Clark, SJ; Harrison, J; Stirzaker, C1
Arnaud, P; Deragon, JM; Goubely, C; Heslop-Harrison, JS; Tatout, C1
Radlińska, M; Skowronek, K1
Klimasauskas, S; Vilkaitis, G1
Jost, JP; Oakeley, EJ; Schmitt, F1
Clark, SJ; Grunau, C; Rosenthal, A1
Holt, BJ; Holt, PG; Watt, PM; White, GP1
Clark, SJ; Grunau, C; Melki, JR; Song, J; Stirzaker, C; Warnecke, PM1
Grønbaek, K; Guldberg, P; Worm, J1
Esteller, M; Fraga, MF1
Aoyama, T; Ishibe, T; Nagayama, S; Nakamura, T; Nakayama, T; Nishijo, K; Okamoto, T; Toguchida, J; Yasura, K1
Grange, T; Kress, C; Thomassin, H1
Hayatsu, H; Negishi, K; Tsuji, K1
Gustafson, TL; Hoose, SA; Kilgore, JA; Kladde, MP; Porter, W1
Clark, SJ; Frommer, M; Molloy, PL; Statham, A; Stirzaker, C1
Mastronardi, FG; Moscarello, MA; Noor, A; Paton, T; Wood, DD1
Hayatsu, H; Moriyama, K; Negishi, K; Shiraishi, M; Tsuji, K1
Chen, Z; Cokus, SJ; Feng, S; Haudenschild, CD; Jacobsen, SE; Merriman, B; Nelson, SF; Pellegrini, M; Pradhan, S; Zhang, X1
Hayatsu, H; Negishi, K; Shiraishi, M1
Nakatani, K; Oka, Y; Peng, T; Takei, F1
Freitag, M; Pomraning, KR; Smith, KM1
Burden, AF; Genereux, DP; Johnson, WC; Laird, CD; Stöger, R1
Hanna, K; Lyko, F; Pollex, T; Schaefer, M1
Huang, Y; Liu, DR; Pastor, WA; Rao, A; Shen, Y; Tahiliani, M1
Jin, SG; Kadam, S; Pfeifer, GP1
Dunican, D; Meehan, R; Nestor, C; Ruzov, A1
Ai, L; Brown, KD; Darst, RP; Kladde, MP; Pardo, CE1
Chávez, L; Kauder, S; Verdin, E1
Aichinger, E; Köhler, C1
Blair, T; Corrie, SR; Feng, Q; Kiviat, NB; Sova, P; Trau, M1
Iqbal, K; Jin, SG; Pfeifer, GP; Szabó, PE1
Cowley, MJ; Cropley, JE; Li, CC; Martin, DI; Preiss, T; Suter, CM1
Paszkowski, J; Reinders, J1
Preiss, T; Squires, JE1
Acevedo, LG; Jelinek, MA; Sanz, A1
Bartholomew, B; Darst, RP; Gangaraju, VK; Kladde, MP; Nabilsi, NH; Pardo, CE; Pondugula, S1
Balasubramanian, S; Booth, MJ; Branco, MR; Ficz, G; Krueger, F; Oxley, D; Reik, W1
Miller, AK; Schüler, P1
Huang, Y; Pastor, WA; Rao, A; Zepeda-Martínez, JA1
Li, G; Lim, JQ; Ruan, Y; Sung, WK; Tennakoon, C; Wei, CL; Wong, E1
He, C; Hon, GC; Jin, P; Ren, B; Song, CX; Szulwach, KE; Yu, M1
Dai, Q; He, C; Hon, GC; Jin, P; Lu, X; Min, JH; Park, B; Ren, B; Song, CX; Street, CR; Szulwach, KE; Tan, H; Wang, X; Yu, M; Zhang, L1
Bollig-Fischer, A; Cao, X; Chen, CC; Cingolani, P; Coon, M; Garfinkel, MD; Huang, Y; Hudson, ME; Khetani, RS; Land, S; Robinson, GE; Ruden, DM; Sammak, A; Zhong, S1
Calvo, MI; Carrasco, E; Espada, J1
Fu, B; Guo, G; Huang, R; Mao, W; Wang, J; Xing, X; Zhou, X1
Ackerman, SE; Chen, LM; Mandarano, AH; Militello, KT; Valentine, EL1
Hu, D; Jin, L; Min, S; Wang, L; Wang, W; Zhang, C1
Cai, W; Chen, B; Liu, Q; Mao, F; Sun, ZS; Wang, Z; Wu, H; Wu, J; Xie, Q; You, M1
Hahn, MA; Li, AX; Pfeifer, GP; Wu, X1
Bachman, M; Balasubramanian, S; Beck, S; Beraldi, D; Field, SF; Stewart, SK1
Yuan, BF1
Bian, Y; Dong, Y; Liu, B; Liu, C; Wang, X; Yang, X; Zhang, H; Zhang, Z; Zhu, B1
Burgess, D; Eichten, SR; Greally, JM; Green, D; Hermanson, PJ; Jeddeloh, J; Li, Q; Patterson, N; Richmond, T; Rosenbaum, H; Springer, NM; Suzuki, M; Wendt, J1
Chung, DH; Groom, J; He, C; Ji, L; Neumann, DA; Schmitz, RJ; Westpheling, J; Yu, M1
Han, D; He, C; Jiang, X; Liu, M; Lu, X; Sun, Z; Xia, B; Xie, W; Yi, C; Yin, Q; Zeng, H; Zhou, A1
Fukuzawa, S; Suetake, I; Tachibana, K; Tajima, S1
Al-Sarraj, S; Burrage, J; Dempster, E; Hannon, E; Kepa, A; Lunnon, K; Mill, J; Schalkwyk, L; Smith, RG; Troakes, C; Wong, C1
Bianchessi, V; Capogrossi, MC; Farina, F; Gualdi, V; Lauri, A; Nigro, P; Pompilio, G; Rizzi, V; Vinci, MC1
Balasubramanian, S; Beraldi, D; Billker, O; McInroy, GR; Modrzynska, K; Raiber, EA; van Delft, P1
Freeman, WM; Giles, CB; Hadad, N; Masser, DR; Richardson, A; Sonntag, WE; Stanford, DR; Wren, JD1
Axelsson, T; Barragan, I; Ewels, P; Ingelman-Sundberg, M; Ivanov, M; Käller, M; Kals, M; Lauschke, V; Lehtiö, J; Milani, L1
Wu, H; Wu, X; Zhang, Y1
Kisliouk, T; Marco, A; Meiri, N; Tabachnik, T; Weller, A1
Ficz, G; Giehr, P; Kyriakopoulos, C; Walter, J; Wolf, V1
Incarnato, D; Krepelova, A; Neri, F; Oliviero, S; Parlato, C1
Fukuzawa, S; Suetake, I; Tachibana, K; Tajima, S; Takahashi, S1
Aburatani, H; Hayashi, G; Kamio, A; Koyama, K; Nagae, G; Okamoto, A; Shiota, H; Umeda, T1
Bourret, G; Bramoulle, A; Chen, GG; Ehlert, U; Ernst, C; Gardini, ES; Gross, JA; Lepage, P; Lutz, PE; Masurel, A; Maussion, G; Mechawar, N; Théroux, JF; Turecki, G; Vaillancourt, K1
Clark, SJ; Gould, CM; Luu, PL; Nair, SS; Skvortsova, K; Stirzaker, C; Zotenko, E1
Moran, S1
Adey, A; Eils, R; Plass, C; Shendure, J; Wang, Q; Weichenhan, D; Wolf, S1
Peat, JR; Smallwood, SA1
Giehr, P; Walter, J1
Han, D; He, C; Hon, GC; Yu, M1
Chandra, T; Green, AR; Kirschner, K; Krueger, F1
Amrutkar, SM; Shao, F; Wu, Q1
Bu, R; Gao, G; Jia, Z; Ren, Y; Shi, Y; Wang, T; Xing, L; Zhang, B; Zhang, L1
DiStefano, JK; Leti, F; Llaci, L; Malenica, I1
Gopalan, V; Islam, F; Lam, AK; Tang, JC1
Giehr, P; Kyriakopoulos, C; Lepikhov, K; Wallner, S; Walter, J; Wolf, V1
Chung, TH; Jia, XY; Sun, X; Tan, D1
Edwards, JR; Gabel, HW; Schlosberg, CE; Wu, DY1
Bai, C; Bi, Y; Chen, L; Liu, Y; Schuster-Böckler, B; Siejka-Zielińska, P; Song, CX; Tomkova, M; Velikova, G; Yuan, F1
Tang, L1
Benner, A; Slynko, A1
Kriegel, AJ; Liang, M; Liu, Y1
Singer, BD1
Gouil, Q; Keniry, A1
An, Z; Li, F; Li, J; Liu, J; Luo, J; Zhang, Z1
Cui, X; Dai, Q; Dou, X; Gao, Y; He, C; Liu, C; Liu, J; Narkhede, P; Zhang, LS; Zhao, BS1
Gibas, P; Gordevičius, J; Klimašauskas, S; Kriukienė, E; Narmontė, M; Staševskij, Z1
Farrell, C; Fehling, HL; Henkhaus, R; Morselli, M; Pellegrini, M; Rubbi, L1
Huang, Z; Kohli, RM; Meng, Y; Pfeifer, GP; Szabó, PE1
Branco, MR; De Borre, M1
Berrios, KN; Kohli, RM; Luo, M; Schutsky, EK; Wang, T; Wu, H1
Ahmed, AA; Chen, J; Cheng, J; Hu, Z; Inoue, M; Liu, Y; Siejka-Zielińska, P; Song, CX1
Eden, HE; Huang, Z; Robertson, KD; Rothbart, SB; Tiedemann, RL1
Kiihl, SF1
Bogdanovic, O; Skvortsova, K1
Xu, X; Zeisberg, EM1
Burgess, A; David, R; Do, T; Li, J; Ng, PQ; Nguyen, V; Searle, I; Wu, X; Zhao, J1
Akalin, A; Alonso, A; Axelsson, T; Blume, A; Bruinsma, S; Butler, D; Campbell, MA; Cavalcante, R; Chen, Z; Davis, JW; Deng, Y; Foox, J; Franke, V; Golda, G; Gong, T; Greally, JM; Guo, S; Gurvitch, J; Hong, H; Ibanez de Caceres, I; Ishii, J; Lacey, M; Lalancette, C; Langhorst, BW; Lent, S; Li, J; Liao, W; Liljedahl, U; Lundmark, A; Mason, CE; Megherbi, DB; Melnick, A; Menor, M; Mozsary, C; Muszynska, A; Nasu, M; Ning, B; Nordlund, J; Padmanabhan, KR; Ponnaluri, VKC; Raine, A; Rodriguez-Antolin, C; Rosas, R; Scherer, A; Sheridan, C; Suzuki, M; Tong, W; Wang, C; Wang, H; Wang, Y; Williams, L; Wos, F; Xiao, W; Xu, J; Yang, X; Yang, Z; Łabaj, PP1
Angeloni, A; Bogdanovic, O; Ferguson, J1
Chen, KK; Feng, YQ; Ji, TT; Tang, F; Xie, C; Xie, NB; Xiong, J; Yu, SY; Yuan, BF1
Chen, KK; Feng, YQ; Guo, X; Ji, TT; Tang, F; Wang, M; Xie, C; Xie, NB; Xiong, J; Yu, SY; Yuan, BF1
Chen, SY; Huang, PH1
Han, Y; Shao, Z; Zhou, D1
An, R; Bai, Y; Cao, Y; Fan, Y; Gu, Q; Peng, J; Ren, L; Song, L; Song, W; Xie, XS; Yuan, T; Zheng, Y1

Reviews

10 review(s) available for 5-methylcytosine and sulfites

ArticleYear
Sequencing 5-methylcytosine residues in genomic DNA.
    BioEssays : news and reviews in molecular, cellular and developmental biology, 1994, Volume: 16, Issue:6

    Topics: 5-Methylcytosine; Base Sequence; Blotting, Southern; Cytosine; DNA; DNA Restriction Enzymes; Electrophoresis, Polyacrylamide Gel; Genome; Hydrazines; Methylation; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

1994
Sequencing 5-methylcytosine residues by the bisulphite method.
    DNA sequence : the journal of DNA sequencing and mapping, 1996, Volume: 6, Issue:4

    Topics: 5-Methylcytosine; Animals; Automation; Base Sequence; Cytosine; DNA; Indicators and Reagents; Nucleic Acid Denaturation; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Sulfites

1996
DNA methylation: a profile of methods and applications.
    BioTechniques, 2002, Volume: 33, Issue:3

    Topics: 5-Methylcytosine; Chromatography, High Pressure Liquid; Cytosine; DNA; DNA Methylation; Electrophoresis, Capillary; Genome; Microchemistry; Polymerase Chain Reaction; Restriction Mapping; Sequence Analysis, DNA; Sulfites

2002
In vivo, in vitro, and in silico analysis of methylation of the HIV-1 provirus.
    Methods (San Diego, Calif.), 2011, Volume: 53, Issue:1

    Topics: 5-Methylcytosine; Base Sequence; Cloning, Molecular; Computer Simulation; CpG Islands; DNA Methylation; DNA, Viral; Genome, Viral; HIV Infections; HIV-1; Humans; Models, Genetic; Proviruses; Sulfites; Virus Integration; Virus Latency

2011
Bisulfite methylation profiling of large genomes.
    Epigenomics, 2010, Volume: 2, Issue:2

    Topics: 5-Methylcytosine; Animals; Arabidopsis; Cytosine; DNA Methylation; Epigenesis, Genetic; Genome; High-Throughput Nucleotide Sequencing; Humans; Mice; Nanopores; Sulfites

2010
Novel DNA binding domain-based assays for detection of methylated and nonmethylated DNA.
    Epigenomics, 2011, Volume: 3, Issue:1

    Topics: 5-Methylcytosine; Biomarkers; CpG Islands; Cytosine; DNA (Cytosine-5-)-Methyltransferases; DNA Methylation; DNA Primers; Humans; Immunoprecipitation; Polymerase Chain Reaction; Protein Interaction Domains and Motifs; Sensitivity and Specificity; Sulfites

2011
5-methylcytosine and its derivatives.
    Advances in clinical chemistry, 2014, Volume: 67

    Topics: 5-Methylcytosine; Chromatography, Thin Layer; Cytosine; DNA Methylation; Electrophoresis, Capillary; Endonucleases; Epigenesis, Genetic; Gas Chromatography-Mass Spectrometry; Humans; Microarray Analysis; Oxidation-Reduction; Polymerase Chain Reaction; Sulfites

2014
Technologies for Deciphering Epigenomic DNA Patterns.
    Advances in experimental medicine and biology, 2017, Volume: 978

    Topics: 5-Methylcytosine; CpG Islands; Cytosine; DNA Methylation; Epigenomics; Formaldehyde; Humans; Microarray Analysis; Paraffin Embedding; Sulfites; Tissue Fixation; Uracil

2017
Methods for CpG Methylation Array Profiling Via Bisulfite Conversion.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1706

    Topics: 5-Methylcytosine; Animals; CpG Islands; DNA Methylation; Humans; Oligonucleotide Array Sequence Analysis; Sequence Analysis, DNA; Sulfites

2018
Latest techniques to study DNA methylation.
    Essays in biochemistry, 2019, 12-20, Volume: 63, Issue:6

    Topics: 5-Methylcytosine; Adenine; Animals; DNA; DNA Methylation; Epigenomics; Humans; Nanopores; Sequence Analysis, DNA; Sulfites

2019

Other Studies

114 other study(ies) available for 5-methylcytosine and sulfites

ArticleYear
Reaction of bisulfite with the 5-hydroxymethyl group in pyrimidines and in phage DNAs.
    Biochemistry, 1979, Feb-20, Volume: 18, Issue:4

    Topics: 5-Methylcytosine; Chemical Phenomena; Chemistry; Coliphages; Cytosine; DNA, Viral; Pentoxyl; Pyrimidines; Spectrophotometry, Ultraviolet; Sulfites; Uracil

1979
A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.
    Proceedings of the National Academy of Sciences of the United States of America, 1992, Mar-01, Volume: 89, Issue:5

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA; Humans; Kininogens; Methylation; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction; Promoter Regions, Genetic; Sulfites

1992
Direct determination of uracil in [32P,uracil-3H]poly(dA.dT) and bisulfite-treated phage PM2 DNA.
    Analytical biochemistry, 1984, Nov-01, Volume: 142, Issue:2

    Topics: 5-Methylcytosine; Bacteriophages; Chromatography, Thin Layer; Cytosine; Deoxyuracil Nucleotides; DNA; DNA Glycosylases; DNA, Viral; Humans; N-Glycosyl Hydrolases; Placenta; Poly dA-dT; Polydeoxyribonucleotides; Polyethyleneimine; Sulfites; Uracil; Uracil-DNA Glycosidase

1984
CpNpG methylation in mammalian cells.
    Nature genetics, 1995, Volume: 10, Issue:1

    Topics: 3T3 Cells; 5-Methylcytosine; Animals; Base Sequence; Cell Line; Cytosine; DNA; DNA Restriction Enzymes; Methylation; Methyltransferases; Mice; Molecular Sequence Data; Mutagens; Plasmids; Sequence Analysis; Sulfites; Transfection

1995
A bisulfite method of 5-methylcytosine mapping that minimizes template degradation.
    Analytical biochemistry, 1995, Mar-20, Volume: 226, Issue:1

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA; DNA Modification Methylases; DNA Primers; Hydrogen-Ion Concentration; Methylation; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Templates, Genetic

1995
High sensitivity mapping of methylated cytosines.
    Nucleic acids research, 1994, Aug-11, Volume: 22, Issue:15

    Topics: 5-Methylcytosine; Base Sequence; Cloning, Molecular; Cytosine; DNA Primers; DNA, Single-Stranded; Hydrogen-Ion Concentration; Kinetics; Methylation; Molecular Sequence Data; Nucleic Acid Denaturation; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Uracil

1994
Methylation analysis on individual chromosomes: improved protocol for bisulphite genomic sequencing.
    Nucleic acids research, 1994, Feb-25, Volume: 22, Issue:4

    Topics: 5-Methylcytosine; Animals; Chromosomes; Cytosine; DNA; Genome; Methylation; Mice; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

1994
An improved method for detection of 5-methylcytosine by PCR-based genomic sequencing.
    BioTechniques, 1994, Volume: 16, Issue:3

    Topics: 5-Methylcytosine; Animals; Base Sequence; Cytosine; DNA; Hydrogen-Ion Concentration; Methylation; Molecular Sequence Data; Nucleic Acid Denaturation; Polymerase Chain Reaction; Sulfites

1994
Methylation pattern of Activator transposase binding sites in maize endosperm.
    The Plant cell, 1996, Volume: 8, Issue:4

    Topics: 5-Methylcytosine; Base Sequence; Chromatids; Cytosine; DNA; DNA Primers; DNA Transposable Elements; Methylation; Molecular Sequence Data; Recombination, Genetic; Sulfites; Zea mays

1996
Active mammalian replication origins are associated with a high-density cluster of mCpG dinucleotides.
    Molecular and cellular biology, 1997, Volume: 17, Issue:1

    Topics: 5-Methylcytosine; Animals; CHO Cells; CpG Islands; Cricetinae; Cytosine; Dinucleoside Phosphates; DNA; DNA Methylation; Molecular Sequence Data; Replication Origin; Ribosomal Proteins; Sulfites

1997
Imprinted segments in the human genome: different DNA methylation patterns in the Prader-Willi/Angelman syndrome region as determined by the genomic sequencing method.
    Human molecular genetics, 1997, Volume: 6, Issue:3

    Topics: 5-Methylcytosine; Angelman Syndrome; Autoantigens; Chromosomes, Human, Pair 15; Cloning, Molecular; CpG Islands; Cytosine; Dinucleoside Phosphates; DNA Methylation; Female; Genomic Imprinting; Humans; Male; Plasmids; Polymerase Chain Reaction; Prader-Willi Syndrome; Promoter Regions, Genetic; Restriction Mapping; Ribonucleoproteins, Small Nuclear; Sequence Analysis, DNA; Sequence Deletion; snRNP Core Proteins; Sulfites

1997
Use of a single sequencing termination reaction to distinguish between cytosine and 5-methylcytosine in bisulfite-modified DNA.
    BioTechniques, 1997, Volume: 22, Issue:5

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA; DNA Methylation; DNA Primers; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

1997
Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA.
    Nucleic acids research, 1997, Nov-01, Volume: 25, Issue:21

    Topics: 5-Methylcytosine; Animals; Base Composition; Cell Line; Cytosine; DNA; DNA Methylation; DNA Primers; Genes, Retinoblastoma; Humans; Mice; Polymerase Chain Reaction; Sensitivity and Specificity; Sulfites

1997
Urea improves efficiency of bisulphite-mediated sequencing of 5'-methylcytosine in genomic DNA.
    Nucleic acids research, 1998, Nov-01, Volume: 26, Issue:21

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA, Single-Stranded; Genes, MDR; Genome, Human; Humans; Indicators and Reagents; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Urea

1998
Cytosines adjacent to methylated CpG sites can be partially resistant to conversion in genomic bisulfite sequencing leading to methylation artifacts.
    Analytical biochemistry, 1998, Nov-01, Volume: 264, Issue:1

    Topics: 5-Methylcytosine; Artifacts; CpG Islands; Cytosine; DNA Methylation; Sequence Analysis, DNA; Sulfites

1998
S1 SINE retroposons are methylated at symmetrical and non-symmetrical positions in Brassica napus: identification of a preferred target site for asymmetrical methylation.
    Plant molecular biology, 1999, Volume: 39, Issue:2

    Topics: 5-Methylcytosine; Brassica; Cytosine; DNA Methylation; DNA, Plant; In Situ Hybridization, Fluorescence; Short Interspersed Nucleotide Elements; Sulfites

1999
Novel procedure for the detection of 5-methylcytosine.
    Acta microbiologica Polonica, 1998, Volume: 47, Issue:4

    Topics: 5-Methylcytosine; Base Composition; Cytosine; DNA; DNA Methylation; DNA Modification Methylases; Genetic Techniques; Molecular Sequence Data; Plasmids; Sequence Analysis, DNA; Sulfites

1998
Bisulfite sequencing protocol displays both 5-methylcytosine and N4-methylcytosine.
    Analytical biochemistry, 1999, Jun-15, Volume: 271, Issue:1

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA; DNA Methylation; DNA Primers; DNA, Bacterial; Escherichia coli; Plasmids; Sequence Analysis, DNA; Sulfites

1999
Quantification of 5-methylcytosine in DNA by the chloroacetaldehyde reaction.
    BioTechniques, 1999, Volume: 27, Issue:4

    Topics: 5-Methylcytosine; Acetaldehyde; Borohydrides; Chemical Precipitation; Chromatography, High Pressure Liquid; Cinnamates; Cytosine; DNA; DNA Methylation; Fluorescent Dyes; Hydrogen-Ion Concentration; Hydrolysis; Hygromycin B; Purines; Silver Nitrate; Solutions; Spectrometry, Fluorescence; Sulfites

1999
Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.
    Nucleic acids research, 2001, Jul-01, Volume: 29, Issue:13

    Topics: 5-Methylcytosine; Amination; Bacteriophage M13; Base Sequence; Chromatography, High Pressure Liquid; Cloning, Molecular; Cytosine; DNA; DNA Methylation; DNA Primers; DNA, Single-Stranded; DNA, Viral; Genome; Molecular Sequence Data; Nucleic Acid Denaturation; Plasmids; Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA; Sulfites; Temperature; Templates, Genetic; Uracil

2001
Differential patterns of methylation of the IFN-gamma promoter at CpG and non-CpG sites underlie differences in IFN-gamma gene expression between human neonatal and adult CD45RO- T cells.
    Journal of immunology (Baltimore, Md. : 1950), 2002, Mar-15, Volume: 168, Issue:6

    Topics: 5-Methylcytosine; Adenine; Adult; Calcium-Binding Proteins; CD3 Complex; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cloning, Molecular; CpG Islands; Cytosine; Dinucleotide Repeats; DNA Methylation; DNA Methyltransferase 3A; DNA-Binding Proteins; Fetal Blood; Gene Expression Regulation; Humans; Infant, Newborn; Interferon-gamma; Interleukin-4; Leukocyte Common Antigens; Microfilament Proteins; Promoter Regions, Genetic; Sequence Analysis, DNA; Sulfites; T-Lymphocyte Subsets; Thymine; Tumor Necrosis Factor-alpha

2002
Identification and resolution of artifacts in bisulfite sequencing.
    Methods (San Diego, Calif.), 2002, Volume: 27, Issue:2

    Topics: 5-Methylcytosine; Artifacts; Cytosine; DNA Primers; False Positive Reactions; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

2002
Profiling DNA methylation by melting analysis.
    Methods (San Diego, Calif.), 2002, Volume: 27, Issue:2

    Topics: 5-Methylcytosine; Cytosine; DNA Methylation; Hot Temperature; Nucleic Acid Denaturation; Polymerase Chain Reaction; Sulfites; Thermodynamics

2002
Methylation in the core-promoter region of the chondromodulin-I gene determines the cell-specific expression by regulating the binding of transcriptional activator Sp3.
    The Journal of biological chemistry, 2004, Jul-02, Volume: 279, Issue:27

    Topics: 5-Methylcytosine; Azacitidine; Binding Sites; Blotting, Western; Bone and Bones; Cartilage; Cell Line, Tumor; Chondrocytes; Chromatin; CpG Islands; Cytosine; Decitabine; DNA Methylation; DNA-Binding Proteins; Genes, Reporter; Humans; Intercellular Signaling Peptides and Proteins; Luciferases; Membrane Proteins; Precipitin Tests; Promoter Regions, Genetic; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA, Messenger; RNA, Small Interfering; Sp1 Transcription Factor; Sp3 Transcription Factor; Sulfites; Thymine; Transcription Factors; Transcription, Genetic

2004
MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome.
    Nucleic acids research, 2004, Dec-02, Volume: 32, Issue:21

    Topics: 5-Methylcytosine; Animals; Benzothiazoles; Cell Line, Tumor; CpG Islands; Diamines; DNA Methylation; DNA Primers; DNA, Neoplasm; Fluorescent Dyes; Genomics; Oligonucleotides; Oligonucleotides, Antisense; Organic Chemicals; Polymerase Chain Reaction; Quinolines; Rats; Sequence Analysis, DNA; Sulfites

2004
Does urea promote the bisulfite-mediated deamination of cytosine in DNA? Investigation aiming at speeding-up the procedure for DNA methylation analysis.
    Nucleic acids symposium series (2004), 2006, Issue:50

    Topics: 5-Methylcytosine; Cell Line, Tumor; Cytosine; Deamination; DNA; DNA Methylation; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Urea

2006
Single-molecule and population probing of chromatin structure using DNA methyltransferases.
    Methods (San Diego, Calif.), 2007, Volume: 41, Issue:3

    Topics: 5-Methylcytosine; Cell Nucleus; Cells, Cultured; Chromatin Assembly and Disassembly; Cloning, Molecular; CpG Islands; DNA Methylation; DNA Modification Methylases; DNA Primers; Genes, Tumor Suppressor; Genome; Genome, Fungal; Humans; Molecular Biology; Nucleosomes; Polymerase Chain Reaction; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sulfites

2007
DNA methylation: bisulphite modification and analysis.
    Nature protocols, 2006, Volume: 1, Issue:5

    Topics: 5-Methylcytosine; Animals; DNA Methylation; Genome, Human; Humans; Sequence Analysis, DNA; Sulfites

2006
Peptidyl argininedeiminase 2 CpG island in multiple sclerosis white matter is hypomethylated.
    Journal of neuroscience research, 2007, Volume: 85, Issue:9

    Topics: 5-Methylcytosine; Blotting, Western; Brain; Citrulline; CpG Islands; DNA; DNA, Single-Stranded; Fluorescent Antibody Technique; Humans; Hydrolases; Methylation; Multiple Sclerosis; Myelin Basic Protein; Promoter Regions, Genetic; Protein-Arginine Deiminase Type 2; Protein-Arginine Deiminases; Reverse Transcriptase Polymerase Chain Reaction; Sulfites; Thymus Gland

2007
Chemistry of bisulfite genomic sequencing; advances and issues.
    Nucleic acids symposium series (2004), 2007, Issue:51

    Topics: 5-Methylcytosine; Cell Line, Tumor; Cytosine; Deamination; DNA Methylation; Genomics; Humans; Quaternary Ammonium Compounds; Sequence Analysis, DNA; Sulfites; Urea

2007
Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning.
    Nature, 2008, Mar-13, Volume: 452, Issue:7184

    Topics: 5-Methylcytosine; Animals; Arabidopsis; Base Sequence; Computational Biology; Cytosine; DNA Methylation; Gene Expression Regulation, Plant; Gene Library; Genome, Plant; Mice; Mutation; Promoter Regions, Genetic; Reproducibility of Results; Sequence Analysis, DNA; Sulfites; Uracil

2008
Bisulfite modification for analysis of DNA methylation.
    Current protocols in nucleic acid chemistry, 2008, Volume: Chapter 6

    Topics: 5-Methylcytosine; Animals; Base Composition; Base Sequence; Deamination; DNA; DNA Methylation; Genome; Molecular Sequence Data; Sequence Analysis, DNA; Solubility; Solutions; Sulfites

2008
The reaction of cytosine with bisulfite by base flipping from the duplex.
    Nucleic acids symposium series (2004), 2008, Issue:52

    Topics: 5-Methylcytosine; Base Pair Mismatch; Carbamates; Chromatography, High Pressure Liquid; Cytosine; DNA; Hydroxylamine; Naphthyridines; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfites

2008
Genome-wide high throughput analysis of DNA methylation in eukaryotes.
    Methods (San Diego, Calif.), 2009, Volume: 47, Issue:3

    Topics: 5-Methylcytosine; Chemistry Techniques, Analytical; DNA; DNA Methylation; Electronic Data Processing; Eukaryotic Cells; Gene Library; Immunoprecipitation; Neurospora crassa; Reproducibility of Results; Sequence Alignment; Software; Sulfites

2009
Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies.
    Nucleic acids research, 2008, Volume: 36, Issue:22

    Topics: 5-Methylcytosine; Cytosine; DNA; DNA Methylation; DNA, Single-Stranded; Nucleic Acid Conformation; Oligonucleotides; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Temperature

2008
RNA cytosine methylation analysis by bisulfite sequencing.
    Nucleic acids research, 2009, Volume: 37, Issue:2

    Topics: 5-Methylcytosine; Animals; Cytosine; Drosophila melanogaster; Methylation; RNA; RNA, Ribosomal, 16S; RNA, Transfer, Asp; Sequence Analysis, RNA; Sulfites

2009
The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing.
    PloS one, 2010, Jan-26, Volume: 5, Issue:1

    Topics: 5-Methylcytosine; Base Sequence; Chromatography, Liquid; Cytosine; Mass Spectrometry; Molecular Sequence Data; Polymerase Chain Reaction; Sulfites

2010
Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine.
    Nucleic acids research, 2010, Volume: 38, Issue:11

    Topics: 5-Methylcytosine; Antibodies; Cytosine; DNA; DNA Methylation; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Immunoprecipitation; Oligonucleotides; Sequence Analysis, DNA; Sulfites

2010
Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA.
    BioTechniques, 2010, Volume: 48, Issue:4

    Topics: 5-Methylcytosine; CpG Islands; Cytosine; DNA; DNA Methylation; DNA Restriction Enzymes; Genes, BRCA1; Humans; Octamer Transcription Factor-3; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

2010
Bisulfite sequencing of DNA.
    Current protocols in molecular biology, 2010, Volume: Chapter 7

    Topics: 5-Methylcytosine; DNA; Sequence Analysis, DNA; Sulfites

2010
Bisulphite sequencing of plant genomic DNA.
    Methods in molecular biology (Clifton, N.J.), 2010, Volume: 655

    Topics: 5-Methylcytosine; Base Sequence; Cytosine; DNA Methylation; DNA, Plant; DNA, Single-Stranded; Epigenomics; Genome, Plant; Plants; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Uracil

2010
Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay.
    The Analyst, 2011, Feb-21, Volume: 136, Issue:4

    Topics: 5-Methylcytosine; Biosensing Techniques; DNA; DNA Methylation; DNA-Binding Proteins; Flow Cytometry; Genetic Loci; Humans; Microspheres; Nucleic Acid Hybridization; Sulfites

2011
Reprogramming of the paternal genome upon fertilization involves genome-wide oxidation of 5-methylcytosine.
    Proceedings of the National Academy of Sciences of the United States of America, 2011, Mar-01, Volume: 108, Issue:9

    Topics: 5-Methylcytosine; Animals; Cellular Reprogramming; Chromosomal Proteins, Non-Histone; Cleavage Stage, Ovum; Cytosine; DNA-Binding Proteins; Embryo, Mammalian; Female; Fertilization; Gene Expression Regulation, Developmental; Genome; Male; Mice; Oocytes; Oxidation-Reduction; Repressor Proteins; Sequence Analysis, DNA; Sulfites; Zygote

2011
A sustained dietary change increases epigenetic variation in isogenic mice.
    PLoS genetics, 2011, Volume: 7, Issue:4

    Topics: 5-Methylcytosine; Alleles; Animals; CpG Islands; Cytosine; Dietary Supplements; DNA Methylation; Environment; Epigenesis, Genetic; Gene Expression; Genetic Loci; Genetic Variation; Mice; Mice, Inbred C57BL; Phenotype; Principal Component Analysis; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; Stochastic Processes; Sulfites

2011
Function and detection of 5-methylcytosine in eukaryotic RNA.
    Epigenomics, 2010, Volume: 2, Issue:5

    Topics: 5-Methylcytosine; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Humans; Mass Spectrometry; Methyltransferases; Models, Molecular; RNA; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sequence Analysis, DNA; Sulfites; Transcriptome; tRNA Methyltransferases

2010
Simultaneous single-molecule detection of endogenous C-5 DNA methylation and chromatin accessibility using MAPit.
    Methods in molecular biology (Clifton, N.J.), 2012, Volume: 833

    Topics: 5-Methylcytosine; Base Sequence; Chromatin; Chromatin Assembly and Disassembly; Cloning, Molecular; DNA Methylation; Humans; K562 Cells; Molecular Biology; Molecular Sequence Data; Sulfites; Transcription Initiation Site

2012
Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution.
    Science (New York, N.Y.), 2012, May-18, Volume: 336, Issue:6083

    Topics: 5-Methylcytosine; Animals; CpG Islands; Cytosine; DNA; DNA Methylation; Embryonic Stem Cells; Epigenesis, Genetic; Genes, Intracisternal A-Particle; High-Throughput Nucleotide Sequencing; Long Interspersed Nucleotide Elements; Mice; Oxidation-Reduction; Rhenium; Sequence Analysis, DNA; Sulfites; Transcription, Genetic; Uracil

2012
Sequencing the sixth base (5-hydroxymethylcytosine): selective DNA oxidation enables base-pair resolution.
    Angewandte Chemie (International ed. in English), 2012, Oct-22, Volume: 51, Issue:43

    Topics: 5-Methylcytosine; Base Pairing; Cytosine; Deamination; DNA; Oxidation-Reduction; Sequence Analysis, DNA; Sulfites

2012
The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine.
    Nature protocols, 2012, Volume: 7, Issue:10

    Topics: 5-Methylcytosine; Antibodies; Base Sequence; Cytosine; DNA Primers; Genomics; Immunoprecipitation; Molecular Sequence Data; Sulfites

2012
BatMeth: improved mapper for bisulfite sequencing reads on DNA methylation.
    Genome biology, 2012, Oct-03, Volume: 13, Issue:10

    Topics: 5-Methylcytosine; Algorithms; CpG Islands; DNA Methylation; Genome, Human; Genomics; Humans; Sequence Analysis, DNA; Sulfites

2012
Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine.
    Nature protocols, 2012, Volume: 7, Issue:12

    Topics: 5-Methylcytosine; Animals; Chromatography, High Pressure Liquid; Cytosine; DNA-Binding Proteins; Glucosyltransferases; Mice; Polymerase Chain Reaction; Proto-Oncogene Proteins; Sequence Analysis, DNA; Sulfites

2012
Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing.
    Nature communications, 2013, Volume: 4

    Topics: 5-Methylcytosine; Animals; DNA; DNA Methylation; DNA-Binding Proteins; Genome; Glucosyltransferases; Mass Spectrometry; Mice; Oxidation-Reduction; Promoter Regions, Genetic; Proto-Oncogene Proteins; Reproducibility of Results; Sequence Analysis, DNA; Staining and Labeling; Sulfites

2013
Intronic non-CG DNA hydroxymethylation and alternative mRNA splicing in honey bees.
    BMC genomics, 2013, Sep-30, Volume: 14

    Topics: 5-Methylcytosine; Africa; Alternative Splicing; Animals; Bees; Behavior, Animal; CpG Islands; Cytosine; DNA Methylation; Europe; Exons; Gene Expression Regulation; Genes, Insect; Honey; Introns; Reproducibility of Results; RNA Splice Sites; RNA, Messenger; Sequence Analysis, DNA; Sulfites

2013
Standard DNA methylation analysis in mouse epidermis: bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunological detection.
    Methods in molecular biology (Clifton, N.J.), 2014, Volume: 1094

    Topics: 5-Methylcytosine; Animals; Base Sequence; DNA; DNA Methylation; Epidermis; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites; Tail

2014
A novel combined bisulfite UDG assay for selective 5-methylcytosine detection.
    Talanta, 2013, Dec-15, Volume: 117

    Topics: 5-Methylcytosine; Cytosine; DNA; DNA Cleavage; DNA Methylation; Electrophoresis, Polyacrylamide Gel; Enzyme Assays; Humans; Oligodeoxyribonucleotides; Sensitivity and Specificity; Sequence Analysis, DNA; Sodium Hydroxide; Solutions; Sulfites; Uracil; Uracil-DNA Glycosidase

2013
A map of 5-methylcytosine residues in Trypanosoma brucei tRNA revealed by sodium bisulfite sequencing.
    Molecular and biochemical parasitology, 2014, Volume: 193, Issue:2

    Topics: 5-Methylcytosine; Base Sequence; Methylation; Molecular Sequence Data; RNA, Transfer; Sequence Analysis, RNA; Sulfites; Trypanosoma brucei brucei

2014
Is the contribution of cis and trans protonated 5-methylcytosine-SO3(-) isomers equal in the conversion to thymine-SO3(-) under bisulfite conditions? A theoretical perspective.
    Physical chemistry chemical physics : PCCP, 2014, Aug-14, Volume: 16, Issue:30

    Topics: 5-Methylcytosine; Isomerism; Models, Molecular; Protons; Sulfates; Sulfites; Thymine

2014
A Bayesian framework to identify methylcytosines from high-throughput bisulfite sequencing data.
    PLoS computational biology, 2014, Volume: 10, Issue:9

    Topics: 5-Methylcytosine; Algorithms; Bayes Theorem; High-Throughput Nucleotide Sequencing; Humans; Models, Genetic; Sensitivity and Specificity; Sequence Analysis, DNA; Sulfites

2014
Single base resolution analysis of 5-methylcytosine and 5-hydroxymethylcytosine by RRBS and TAB-RRBS.
    Methods in molecular biology (Clifton, N.J.), 2015, Volume: 1238

    Topics: 5-Methylcytosine; Cytosine; Deoxyribonuclease HpaII; Glycosylation; Humans; Oxidation-Reduction; Sequence Analysis, DNA; Sulfites

2015
Accurate measurement of 5-methylcytosine and 5-hydroxymethylcytosine in human cerebellum DNA by oxidative bisulfite on an array (OxBS-array).
    PloS one, 2015, Volume: 10, Issue:2

    Topics: 5-Methylcytosine; Adult; Cerebellum; Cytosine; DNA; DNA Methylation; Humans; Male; Oligonucleotide Array Sequence Analysis; Oxidation-Reduction; Reproducibility of Results; Sulfites

2015
Genetic and epigenetic modifications to the BBAA component of common wheat during its evolutionary history at the hexaploid level.
    Plant molecular biology, 2015, Volume: 88, Issue:1-2

    Topics: 5-Methylcytosine; Biological Evolution; DNA Methylation; Epigenesis, Genetic; Fluorescent Antibody Technique; Genome, Plant; Interphase; Karyotyping; Metaphase; Phenotype; Polymorphism, Genetic; Reproducibility of Results; Sequence Analysis, DNA; Sulfites; Tetraploidy; Triticum

2015
Post-conversion targeted capture of modified cytosines in mammalian and plant genomes.
    Nucleic acids research, 2015, Jul-13, Volume: 43, Issue:12

    Topics: 5-Methylcytosine; Alleles; Animals; Cell Line; Cytosine; DNA Methylation; Genome, Plant; Genomics; High-Throughput Nucleotide Sequencing; Humans; Mice; Polymorphism, Single Nucleotide; Sequence Analysis, DNA; Sulfites

2015
Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing.
    Nucleic acids research, 2015, Dec-02, Volume: 43, Issue:21

    Topics: 5-Methylcytosine; Animals; Cytosine; DNA-Binding Proteins; DNA, Bacterial; Firmicutes; Genome, Bacterial; Mice; Nucleotide Motifs; Proto-Oncogene Proteins; Sequence Analysis, DNA; Sulfites

2015
Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.
    Nature methods, 2015, Volume: 12, Issue:11

    Topics: 5-Methylcytosine; Animals; Cell Line; CpG Islands; Cytosine; DNA Methylation; DNA Primers; Epigenomics; Gene Expression Regulation; Genome; Mice; Mice, Transgenic; Oligonucleotides; Oxygen; Polymerase Chain Reaction; Sequence Analysis, DNA; Stem Cells; Sulfites

2015
Selective oxidation of 5-hydroxymethylcytosine with micelle incarcerated oxidants to determine it at single base resolution.
    Bioorganic & medicinal chemistry letters, 2015, Dec-15, Volume: 25, Issue:24

    Topics: 5-Methylcytosine; Base Sequence; Catalysis; Cytosine; Mass Spectrometry; Micelles; Oxidation-Reduction; Sulfites

2015
Variation in 5-hydroxymethylcytosine across human cortex and cerebellum.
    Genome biology, 2016, Feb-16, Volume: 17

    Topics: 5-Methylcytosine; Cerebellar Cortex; Cerebellum; Cytosine; DNA; DNA Methylation; Epigenesis, Genetic; Genome, Human; Humans; Sequence Analysis, DNA; Sulfites

2016
Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells.
    Mitochondrion, 2016, Volume: 27

    Topics: 5-Methylcytosine; Cytosine; DNA, Intergenic; DNA, Mitochondrial; Endothelial Cells; Humans; Mitochondria; Sequence Analysis, DNA; Sulfites

2016
Enhanced Methylation Analysis by Recovery of Unsequenceable Fragments.
    PloS one, 2016, Volume: 11, Issue:3

    Topics: 5-Methylcytosine; Chromatography, High Pressure Liquid; CpG Islands; DNA; DNA Methylation; Gene Library; Plasmodium berghei; Sequence Analysis, DNA; Sulfites; Tandem Mass Spectrometry

2016
Bisulfite oligonucleotide-capture sequencing for targeted base- and strand-specific absolute 5-methylcytosine quantitation.
    Age (Dordrecht, Netherlands), 2016, Volume: 38, Issue:3

    Topics: 5-Methylcytosine; Aging; Animals; DNA; DNA Methylation; Epigenesis, Genetic; Rats; Sequence Analysis, DNA; Sulfites

2016
Single base resolution analysis of 5-hydroxymethylcytosine in 188 human genes: implications for hepatic gene expression.
    Nucleic acids research, 2016, 08-19, Volume: 44, Issue:14

    Topics: 5-Methylcytosine; Adult; Base Pairing; Base Sequence; Chromatography, Liquid; CpG Islands; DNA; Gene Expression Regulation; Genes; Humans; Liver; Mass Spectrometry; Reproducibility of Results; Sequence Analysis, DNA; Sulfites

2016
Base-resolution profiling of active DNA demethylation using MAB-seq and caMAB-seq.
    Nature protocols, 2016, Volume: 11, Issue:6

    Topics: 5-Methylcytosine; Animals; Cell Line; Cytosine; DNA; DNA Methylation; DNA Modification Methylases; Mice; Sequence Analysis, DNA; Sulfites

2016
DNA CpG Methylation (5-Methylcytosine) and Its Derivative (5-Hydroxymethylcytosine) Alter Histone Posttranslational Modifications at the Pomc Promoter, Affecting the Impact of Perinatal Diet on Leanness and Obesity of the Offspring.
    Diabetes, 2016, Volume: 65, Issue:8

    Topics: 5-Methylcytosine; Animals; Brain; CpG Islands; Diet, High-Fat; DNA Methylation; Histones; Hypothalamus; Obesity; Pro-Opiomelanocortin; Promoter Regions, Genetic; Rats; Rats, Wistar; Sulfites; Thinness

2016
The Influence of Hydroxylation on Maintaining CpG Methylation Patterns: A Hidden Markov Model Approach.
    PLoS computational biology, 2016, Volume: 12, Issue:5

    Topics: 5-Methylcytosine; Animals; Cell Division; Computational Biology; CpG Islands; DNA Methylation; DNA Replication; Embryonic Stem Cells; Hydroxylation; Markov Chains; Mice; Models, Biological; Stochastic Processes; Sulfites

2016
Methylation-assisted bisulfite sequencing to simultaneously map 5fC and 5caC on a genome-wide scale for DNA demethylation analysis.
    Nature protocols, 2016, Volume: 11, Issue:7

    Topics: 5-Methylcytosine; Animals; Base Sequence; Cell Line; CpG Islands; Cytosine; DNA; DNA Methylation; Epigenesis, Genetic; Humans; Mice; Sequence Analysis, DNA; Sulfites

2016
Simple and accurate single base resolution analysis of 5-hydroxymethylcytosine by catalytic oxidative bisulfite sequencing using micelle incarcerated oxidants.
    Bioorganic & medicinal chemistry, 2016, 09-15, Volume: 24, Issue:18

    Topics: 5-Methylcytosine; Adamantane; Animals; Cyclic N-Oxides; Cytosine; DNA, Single-Stranded; Embryonic Stem Cells; Iodobenzenes; Mice; Micelles; Onium Compounds; Oxidants; Oxidation-Reduction; Sequence Analysis, DNA; Sodium Dodecyl Sulfate; Sulfites; Temperature

2016
Base-Resolution Analysis of 5-Hydroxymethylcytosine by One-Pot Bisulfite-Free Chemical Conversion with Peroxotungstate.
    Journal of the American Chemical Society, 2016, 11-02, Volume: 138, Issue:43

    Topics: 5-Methylcytosine; Base Pairing; Base Sequence; DNA; Selenium Radioisotopes; Sulfites; Tungsten Compounds

2016
Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine.
    BMC genomics, 2017, 01-18, Volume: 18, Issue:1

    Topics: 5-Methylcytosine; Adult; DNA Methylation; Humans; Male; Oxidation-Reduction; Sequence Analysis, DNA; Sulfites

2017
Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA.
    Epigenetics & chromatin, 2017, Volume: 10

    Topics: 5-Methylcytosine; Brain; Cell Line, Tumor; CpG Islands; DNA; DNA Methylation; Gene Expression Profiling; Genome, Human; Humans; Immunoprecipitation; Mixed Function Oxygenases; Oligonucleotide Array Sequence Analysis; Oxidation-Reduction; Proto-Oncogene Proteins; Sequence Analysis, DNA; Sulfites; Whole Genome Sequencing

2017
Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencing.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1708

    Topics: 5-Methylcytosine; Animals; CpG Islands; DNA Methylation; Epigenesis, Genetic; High-Throughput Nucleotide Sequencing; Humans; Mice; Regulatory Sequences, Nucleic Acid; Sulfites; Whole Genome Sequencing

2018
Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging Approach.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1708

    Topics: 5-Methylcytosine; Animals; CpG Islands; DNA Methylation; Epigenesis, Genetic; Gene Library; High-Throughput Nucleotide Sequencing; Mice; Sample Size; Sulfites; Whole Genome Sequencing

2018
Hairpin Bisulfite Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual Chromosomes.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1708

    Topics: 5-Methylcytosine; Chromosomes; DNA Methylation; DNA, Complementary; Epigenesis, Genetic; High-Throughput Nucleotide Sequencing; Humans; Nucleic Acid Conformation; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

2018
Tet-Assisted Bisulfite Sequencing (TAB-seq).
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1708

    Topics: 5-Methylcytosine; Animals; Cells, Cultured; DNA-Binding Proteins; Epigenomics; Glucosyltransferases; Humans; Mice; Proto-Oncogene Proteins; Recombinant Proteins; Sequence Analysis, DNA; Sulfites

2018
Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq).
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1708

    Topics: 5-Methylcytosine; DNA Methylation; Genomic Library; Humans; Organ Specificity; Sequence Analysis, DNA; Sulfites; Workflow

2018
Sulfinate Based Selective Labeling of 5-Hydroxymethylcytosine: Application to Biotin Pull Down Assay.
    Bioconjugate chemistry, 2018, 02-21, Volume: 29, Issue:2

    Topics: 5-Methylcytosine; Biotin; Biotinylation; DNA; Sulfinic Acids; Sulfites

2018
DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA.
    BMC biotechnology, 2018, 02-06, Volume: 18, Issue:1

    Topics: 5-Methylcytosine; CpG Islands; DNA Methylation; False Positive Reactions; High-Throughput Nucleotide Sequencing; Humans; Immunoprecipitation; Leukocytes, Mononuclear; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; Sulfites

2018
Epigenetics: DNA Methylation Analysis in Esophageal Adenocarcinoma.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1756

    Topics: 5-Methylcytosine; Adenocarcinoma; DNA; DNA Methylation; Epigenesis, Genetic; Esophageal Neoplasms; Esophagus; High-Throughput Nucleotide Sequencing; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA; Sulfites

2018
Two are better than one: HPoxBS - hairpin oxidative bisulfite sequencing.
    Nucleic acids research, 2018, 09-06, Volume: 46, Issue:15

    Topics: 5-Methylcytosine; Animals; Cytosine; DNA; DNA (Cytosine-5-)-Methyltransferase 1; DNA Methylation; DNA-Binding Proteins; Embryonic Stem Cells; Gene Expression Regulation; High-Throughput Nucleotide Sequencing; Mice; Oxidation-Reduction; Proto-Oncogene Proteins; Sulfites

2018
Mirror Bisulfite Sequencing: A Method for Single-Base Resolution of Hydroxymethylcytosine.
    Analytical chemistry, 2018, 11-20, Volume: 90, Issue:22

    Topics: 5-Methylcytosine; Base Sequence; DNA; DNA-Cytosine Methylases; Gene Library; Glucosyltransferases; Humans; Reproducibility of Results; Sensitivity and Specificity; Sequence Analysis, DNA; Sulfites

2018
ME-Class2 reveals context dependent regulatory roles for 5-hydroxymethylcytosine.
    Nucleic acids research, 2019, 03-18, Volume: 47, Issue:5

    Topics: 5-Methylcytosine; Animals; Brain; Databases, Genetic; Datasets as Topic; Genes; Genome; Humans; Machine Learning; Methylation; Mice; Organ Specificity; Promoter Regions, Genetic; Sequence Analysis, RNA; Sulfites

2019
Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution.
    Nature biotechnology, 2019, Volume: 37, Issue:4

    Topics: 5-Methylcytosine; Animals; Base Sequence; Biotechnology; CpG Islands; DNA; DNA Methylation; Humans; Mice; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfites; Whole Genome Sequencing

2019
Bisulfite-free epigenetic sequencing.
    Nature methods, 2019, Volume: 16, Issue:4

    Topics: 5-Methylcytosine; Sequence Analysis, DNA; Sulfites

2019
Statistical methods for classification of 5hmC levels based on the Illumina Inifinium HumanMethylation450 (450k) array data, under the paired bisulfite (BS) and oxidative bisulfite (oxBS) treatment.
    PloS one, 2019, Volume: 14, Issue:6

    Topics: 5-Methylcytosine; Case-Control Studies; DNA Methylation; Humans; Neoplasms; Oxidation-Reduction; Sulfites

2019
Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Adapter.
    Methods in molecular biology (Clifton, N.J.), 2019, Volume: 2018

    Topics: 5-Methylcytosine; Animals; CpG Islands; Cytosine; DNA Methylation; DNA Primers; Gene Library; Rats; Sequence Analysis, DNA; Sulfites

2019
A Practical Guide to the Measurement and Analysis of DNA Methylation.
    American journal of respiratory cell and molecular biology, 2019, Volume: 61, Issue:4

    Topics: 5-Methylcytosine; Base Sequence; Computational Biology; CpG Islands; DNA; DNA (Cytosine-5-)-Methyltransferase 1; DNA Methylation; High-Throughput Nucleotide Sequencing; Immunoprecipitation; Methylation; Molecular Structure; Nucleic Acid Hybridization; Quality Control; Sequence Alignment; Sulfites

2019
Episo: quantitative estimation of RNA 5-methylcytosine at isoform level by high-throughput sequencing of RNA treated with bisulfite.
    Bioinformatics (Oxford, England), 2020, 04-01, Volume: 36, Issue:7

    Topics: 5-Methylcytosine; Animals; High-Throughput Nucleotide Sequencing; Humans; Mice; Protein Isoforms; RNA; Sequence Analysis, RNA; Software; Sulfites

2020
DNA 5-Methylcytosine-Specific Amplification and Sequencing.
    Journal of the American Chemical Society, 2020, 03-11, Volume: 142, Issue:10

    Topics: 5-Methylcytosine; Animals; DNA; DNA Methylation; Humans; Mice; Nucleic Acid Amplification Techniques; Sequence Analysis, DNA; Sulfites; Whole Genome Sequencing

2020
Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing.
    PLoS biology, 2020, Volume: 18, Issue:4

    Topics: 5-Methylcytosine; Acetylation; Animals; Bacteriophage lambda; Cell Line; DNA Methylation; Embryonic Stem Cells; Genome; Histones; Lysine; Mice; Oligonucleotides; Reproducibility of Results; Sequence Analysis, DNA; Sulfites

2020
Targeted bisulfite sequencing for biomarker discovery.
    Methods (San Diego, Calif.), 2021, Volume: 187

    Topics: 5-Methylcytosine; Age Factors; Aging; Biomarkers; DNA Methylation; Epigenesis, Genetic; Epigenomics; High-Throughput Nucleotide Sequencing; Humans; Models, Genetic; Sequence Analysis, DNA; Sulfites

2021
High-Resolution Analysis of 5-Hydroxymethylcytosine by TET-Assisted Bisulfite Sequencing.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2198

    Topics: 5-Methylcytosine; Animals; Cytosine; Dioxygenases; DNA; DNA Methylation; Epigenesis, Genetic; Epigenomics; Genome; Humans; Oxidation-Reduction; Sequence Analysis, DNA; Sulfites

2021
Oxidative Bisulfite Sequencing: An Experimental and Computational Protocol.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2198

    Topics: 5-Methylcytosine; Animals; Computational Biology; Cytosine; Dioxygenases; DNA; DNA Methylation; High-Throughput Nucleotide Sequencing; Humans; Oxidation-Reduction; Oxidative Stress; Sequence Analysis, DNA; Sulfites

2021
Bisulfite-Free Sequencing of 5-Hydroxymethylcytosine with APOBEC-Coupled Epigenetic Sequencing (ACE-Seq).
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2198

    Topics: 5-Methylcytosine; Animals; Computational Biology; Cytidine Deaminase; Cytosine; DNA; DNA Methylation; Epigenomics; Humans; Proteins; Sequence Analysis, DNA; Single Molecule Imaging; Sulfites

2021
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution.
    Nature communications, 2021, 01-27, Volume: 12, Issue:1

    Topics: 5-Methylcytosine; Animals; Base Sequence; Mice; Mouse Embryonic Stem Cells; Oxidation-Reduction; Pyridines; Sequence Analysis, DNA; Sulfites

2021
Distinguishing Active Versus Passive DNA Demethylation Using Illumina MethylationEPIC BeadChip Microarrays.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2272

    Topics: 5-Methylcytosine; Computational Biology; Cytosine; DNA; DNA Demethylation; DNA Methylation; Epigenesis, Genetic; High-Throughput Nucleotide Sequencing; Humans; Microarray Analysis; Oxidation-Reduction; Sulfites

2021
Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2272

    Topics: 5-Methylcytosine; Computational Biology; DNA; DNA Methylation; Epigenesis, Genetic; High-Throughput Nucleotide Sequencing; Humans; Oxidation-Reduction; Sulfites

2021
TAB-seq and ACE-seq Data Processing for Genome-Wide DNA hydroxymethylation Profiling.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2272

    Topics: 5-Methylcytosine; Computational Biology; DNA; DNA Methylation; Epigenesis, Genetic; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Mixed Function Oxygenases; Oxidation-Reduction; Proto-Oncogene Proteins; Sulfites

2021
High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2272

    Topics: 5-Methylcytosine; Chromatin; Computational Biology; CRISPR-Cas Systems; DNA; DNA Methylation; Epigenesis, Genetic; Gene Editing; High-Throughput Nucleotide Sequencing; Humans; Mixed Function Oxygenases; Oxidation-Reduction; Promoter Regions, Genetic; Proto-Oncogene Proteins; Sulfites

2021
Quantitative and Single-Nucleotide Resolution Profiling of RNA 5-Methylcytosine.
    Methods in molecular biology (Clifton, N.J.), 2021, Volume: 2298

    Topics: 5-Methylcytosine; Computational Biology; Nucleotides; Polymerase Chain Reaction; RNA; RNA Processing, Post-Transcriptional; Sequence Analysis, DNA; Sulfites

2021
The SEQC2 epigenomics quality control (EpiQC) study.
    Genome biology, 2021, 12-06, Volume: 22, Issue:1

    Topics: 5-Methylcytosine; Algorithms; CpG Islands; DNA; DNA Methylation; Epigenesis, Genetic; Epigenome; Epigenomics; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Quality Control; Sequence Alignment; Sequence Analysis, DNA; Sulfites; Whole Genome Sequencing

2021
Nanopore Sequencing and Data Analysis for Base-Resolution Genome-Wide 5-Methylcytosine Profiling.
    Methods in molecular biology (Clifton, N.J.), 2022, Volume: 2458

    Topics: 5-Methylcytosine; Data Analysis; DNA Methylation; High-Throughput Nucleotide Sequencing; Nanopore Sequencing; Sequence Analysis, DNA; Sulfites

2022
Bisulfite-Free and Single-Base Resolution Detection of Epigenetic DNA Modification of 5-Methylcytosine by Methyltransferase-Directed Labeling with APOBEC3A Deamination Sequencing.
    Analytical chemistry, 2022, 11-08, Volume: 94, Issue:44

    Topics: 5-Methylcytosine; Animals; Cytosine; Deamination; DNA; DNA Methylation; Epigenesis, Genetic; Humans; Mammals; Methyltransferases; Sequence Analysis, DNA; Sulfites

2022
Engineered APOBEC3C Sequencing Enables Bisulfite-Free and Direct Detection of DNA Methylation at a Single-Base Resolution.
    Analytical chemistry, 2023, 01-17, Volume: 95, Issue:2

    Topics: 5-Methylcytosine; Cytidine Deaminase; Cytosine; DNA; DNA Methylation; Epigenesis, Genetic; Humans; Sequence Analysis, DNA; Sulfites

2023
Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing.
    Journal of visualized experiments : JoVE, 2023, 07-07, Issue:197

    Topics: 5-Methylcytosine; DNA Methylation; High-Throughput Nucleotide Sequencing; RNA; RNA, Messenger; Sequence Analysis, DNA; Sulfites

2023
Optimized bisulfite sequencing analysis reveals the lack of 5-methylcytosine in mammalian mitochondrial DNA.
    BMC genomics, 2023, Aug-04, Volume: 24, Issue:1

    Topics: 5-Methylcytosine; Animals; DNA Methylation; DNA, Mitochondrial; Mammals; Mitochondria; Sequence Analysis, DNA; Sulfites

2023
Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalability.
    Proceedings of the National Academy of Sciences of the United States of America, 2023, Dec-05, Volume: 120, Issue:49

    Topics: 5-Methylcytosine; Animals; Cytosine; DNA Methylation; Mice; Sequence Analysis, DNA; Sulfites

2023