5-hydroxy-2--deoxycytidine and 2--deoxycytidine-5--triphosphate

5-hydroxy-2--deoxycytidine has been researched along with 2--deoxycytidine-5--triphosphate* in 2 studies

Other Studies

2 other study(ies) available for 5-hydroxy-2--deoxycytidine and 2--deoxycytidine-5--triphosphate

Article	Year
The mutations induced by oxidatively damaged nucleotides, 5-formyl-dUTP and 5-hydroxy-dCTP,in Escherichia coli. Nucleic acids research, 1998, Oct-15, Volume: 26, Issue:20 The mutational properties of 5-formyl-2'-deoxyuridine 5'-triphosphate (5-CHO-dUTP) and 5-hydroxy-2'-deoxycytidine 5'-triphosphate (5-OH-dCTP), the major oxidatively damaged pyrimidine nucleotides derived from dTTP and dCTP, respectively, were analyzed by an in vivo assay. 5-CHO-dUTP and 5-OH-dCTP were directly incorporated into Escherichia coli , and their mutagenicities were evaluated by the chromosomal lacI forward mutation assay. The mutation frequencies increased, depending on the dose of these damaged nucleotides, indicating that these nucleotides were incorporated into E.coli and acted as mutagens in vivo . The mutagenicities of 5-CHO-dUTP and 5-OH-dCTP were comparable to that of 8-hydroxy-2'-deoxyguanosine 5'-triphosphate, a major form of dGTP oxidative damage. 5-CHO-dUTP induced G.C to A.T, A.T to G.C and G.C to T.A mutations, and 5-OH-dCTP elicited G.C to A.T, A.T to C.G and G.C to T.A mutations. Topics: Bacterial Proteins; Deoxyadenine Nucleotides; Deoxycytidine; Deoxycytosine Nucleotides; Deoxyguanine Nucleotides; Deoxyuracil Nucleotides; Deoxyuridine; DNA Mutational Analysis; DNA Repair; Escherichia coli; Escherichia coli Proteins; Genome, Bacterial; Lac Repressors; Models, Genetic; Mutagenesis; Nucleic Acid Hybridization; Oxidants; Point Mutation; Repressor Proteins; Sequence Analysis, DNA; Thymine Nucleotides	1998
Reverse chemical mutagenesis: identification of the mutagenic lesions resulting from reactive oxygen species-mediated damage to DNA. Proceedings of the National Academy of Sciences of the United States of America, 1994, Jul-05, Volume: 91, Issue:14 An understanding of the contribution of reactive oxygen species to mutagenesis has been hampered by the vast number of different chemical modifications they cause in DNA. Even though many of these DNA alterations have been catalogued, the identification of specific lesions that cause mutations has depended on testing one modification at a time. In this study we present another approach to identify key mutagenic lesions from a pool of oxidatively modified nucleotides. dCTP was treated with an oxygen radical-generating system containing FeSO4, H2O2, and ascorbic acid. The modification products were separated by reverse-phase and anion-exchange HPLC and then incorporated by human immunodeficiency virus reverse transcriptase into a DNA that contains a target gene for scoring for mutations. One of the mutagenic species isolated was identified as 5-hydroxy-2'-deoxycytidine. It is incorporated efficiently into DNA and causes C-->T transitions in Escherichia coli at a frequency of 2.5%, which is more mutagenic than any previously identified oxidative DNA lesion. Topics: Base Sequence; Deoxycytidine; Deoxycytosine Nucleotides; Deoxyribonucleotides; DNA Damage; DNA, Bacterial; DNA, Viral; Escherichia coli; Gamma Rays; Genes, Bacterial; HIV Reverse Transcriptase; HIV-1; Molecular Sequence Data; Mutagenesis; Mutagens; Oxidation-Reduction; Phosphorus Radioisotopes; Reactive Oxygen Species; RNA-Directed DNA Polymerase	1994

Article

Year

The mutations induced by oxidatively damaged nucleotides, 5-formyl-dUTP and 5-hydroxy-dCTP,in Escherichia coli.

Nucleic acids research, 1998, Oct-15, Volume: 26, Issue:20

The mutational properties of 5-formyl-2'-deoxyuridine 5'-triphosphate (5-CHO-dUTP) and 5-hydroxy-2'-deoxycytidine 5'-triphosphate (5-OH-dCTP), the major oxidatively damaged pyrimidine nucleotides derived from dTTP and dCTP, respectively, were analyzed by an in vivo assay. 5-CHO-dUTP and 5-OH-dCTP were directly incorporated into Escherichia coli , and their mutagenicities were evaluated by the chromosomal lacI forward mutation assay. The mutation frequencies increased, depending on the dose of these damaged nucleotides, indicating that these nucleotides were incorporated into E.coli and acted as mutagens in vivo . The mutagenicities of 5-CHO-dUTP and 5-OH-dCTP were comparable to that of 8-hydroxy-2'-deoxyguanosine 5'-triphosphate, a major form of dGTP oxidative damage. 5-CHO-dUTP induced G.C to A.T, A.T to G.C and G.C to T.A mutations, and 5-OH-dCTP elicited G.C to A.T, A.T to C.G and G.C to T.A mutations.

Topics: Bacterial Proteins; Deoxyadenine Nucleotides; Deoxycytidine; Deoxycytosine Nucleotides; Deoxyguanine Nucleotides; Deoxyuracil Nucleotides; Deoxyuridine; DNA Mutational Analysis; DNA Repair; Escherichia coli; Escherichia coli Proteins; Genome, Bacterial; Lac Repressors; Models, Genetic; Mutagenesis; Nucleic Acid Hybridization; Oxidants; Point Mutation; Repressor Proteins; Sequence Analysis, DNA; Thymine Nucleotides

1998

Reverse chemical mutagenesis: identification of the mutagenic lesions resulting from reactive oxygen species-mediated damage to DNA.

Proceedings of the National Academy of Sciences of the United States of America, 1994, Jul-05, Volume: 91, Issue:14

An understanding of the contribution of reactive oxygen species to mutagenesis has been hampered by the vast number of different chemical modifications they cause in DNA. Even though many of these DNA alterations have been catalogued, the identification of specific lesions that cause mutations has depended on testing one modification at a time. In this study we present another approach to identify key mutagenic lesions from a pool of oxidatively modified nucleotides. dCTP was treated with an oxygen radical-generating system containing FeSO4, H2O2, and ascorbic acid. The modification products were separated by reverse-phase and anion-exchange HPLC and then incorporated by human immunodeficiency virus reverse transcriptase into a DNA that contains a target gene for scoring for mutations. One of the mutagenic species isolated was identified as 5-hydroxy-2'-deoxycytidine. It is incorporated efficiently into DNA and causes C-->T transitions in Escherichia coli at a frequency of 2.5%, which is more mutagenic than any previously identified oxidative DNA lesion.

Topics: Base Sequence; Deoxycytidine; Deoxycytosine Nucleotides; Deoxyribonucleotides; DNA Damage; DNA, Bacterial; DNA, Viral; Escherichia coli; Gamma Rays; Genes, Bacterial; HIV Reverse Transcriptase; HIV-1; Molecular Sequence Data; Mutagenesis; Mutagens; Oxidation-Reduction; Phosphorus Radioisotopes; Reactive Oxygen Species; RNA-Directed DNA Polymerase

1994