5-formylcytosine and dicyanmethane

5-formylcytosine has been researched along with dicyanmethane* in 1 studies

Other Studies

1 other study(ies) available for 5-formylcytosine and dicyanmethane

Article	Year
Bisulfite-free and quantitative detection of 5-formylcytosine in DNA through qPCR. Chemical communications (Cambridge, England), 2021, Dec-16, Volume: 57, Issue:100 An easily operated bisulfite-free method was presented to detect and quantify 5fC through quantitative real-time PCR. Malononitrile can selectively label 5fC under mild reaction conditions causing a C-to-T conversion that affects the nick ligation of the complementary pairing oligos, and then the ligation product is amplified and visualized by qPCR. Topics: Cytosine; DNA; Limit of Detection; Nitriles; Real-Time Polymerase Chain Reaction	2021

Article

Year

Bisulfite-free and quantitative detection of 5-formylcytosine in DNA through qPCR.

Chemical communications (Cambridge, England), 2021, Dec-16, Volume: 57, Issue:100

An easily operated bisulfite-free method was presented to detect and quantify 5fC through quantitative real-time PCR. Malononitrile can selectively label 5fC under mild reaction conditions causing a C-to-T conversion that affects the nick ligation of the complementary pairing oligos, and then the ligation product is amplified and visualized by qPCR.

Topics: Cytosine; DNA; Limit of Detection; Nitriles; Real-Time Polymerase Chain Reaction

2021