5-formyl-2--deoxycytidine and metaperiodate

5-formyl-2--deoxycytidine has been researched along with metaperiodate* in 1 studies

Other Studies

1 other study(ies) available for 5-formyl-2--deoxycytidine and metaperiodate

ArticleYear
Synthesis and properties of oligonucleotides containing 5-formyl-2'-deoxycytidine: in vitro DNA polymerase reactions on DNA templates containing 5-formyl-2'-deoxycytidine.
    Nucleic acids research, 2001, Jun-15, Volume: 29, Issue:12

    Oligodeoxynucleotides (ODNs) containing 5-formyl-2'-deoxycytidine (fC) were synthesized by the phosphoramidite method and subsequent oxidation with sodium periodate. The stabilities of duplexes containing A, G, C or T opposite fC were studied by thermal denaturation. It was found that fC:A, fC:C or fC:T base pairs significantly reduce the thermal stabilities of duplexes. Next, single nucleotide insertion reactions were performed using ODNs containing fC as templates and the Klenow fragment of Escherichia coli DNA polymerase I. It was found that: (i) insertion of dGMP opposite fC appears to be less efficient relative to insertion opposite 5-methyl-2'-deoxycytidine (mC); (ii) dAMP is misincorporated more frequently opposite fC than mC, although the frequency of misincorporation seems to be dependent on the sequence; (iii) TMP is misincorporated more frequently opposite fC than mC. These results suggest that fC may induce the transition mutation C.G-->T.A and the transversion mutation C.G-->A.T during DNA synthesis.

    Topics: Base Pairing; Base Sequence; Deoxycytidine; DNA; DNA Polymerase I; DNA Primers; DNA Replication; Escherichia coli; Kinetics; Magnetic Resonance Spectroscopy; Mutagenesis; Nucleic Acid Denaturation; Nucleic Acid Hybridization; Oligonucleotides; Periodic Acid; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Templates, Genetic; Thermodynamics; Ultraviolet Rays

2001