5-fluoroorotic acid has been researched along with orotic acid in 137 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 21 (15.33) | 18.7374 |
| 1990's | 36 (26.28) | 18.2507 |
| 2000's | 47 (34.31) | 29.6817 |
| 2010's | 31 (22.63) | 24.3611 |
| 2020's | 2 (1.46) | 2.80 |
| Authors | Studies |
|---|---|
| Cheleski, J; da Silva, AB; Montanari, CA; Nonato, MC; Pinheiro, MP; Rocha, JR; Wiggers, HJ | 1 |
| Hsiao, WL; Krooth, RS; Potvin, BW | 1 |
| Krungkrai, J; Krungkrai, SR; Phakanont, K | 1 |
| Leffers, NP; Rathod, PK; Young, RD | 1 |
| Bennett, JE; Edman, JC; Kwon-Chung, KJ; Varma, A | 1 |
| Silar, P; Thiele, DJ | 1 |
| Fujimura, H | 1 |
| Kondo, S; Oshima, T; Yamagishi, A | 1 |
| Gomez, ZM; Rathod, PK | 2 |
| Boy-Marcotte, E; Dingermann, T; Jacquet, M; Kalpaxis, D; Reindl, N; Werner, H; Zündorf, I | 1 |
| de Graaff, LH; Gruber, F; Kubicek, CP; Visser, J | 1 |
| Brown, CS; Heath, TD; Stern, WH | 1 |
| Alvarado, JA | 1 |
| Hubbert, T; Khatri, A; Milhous, WK; Rathod, PK | 1 |
| Christman, MF; Dietrich, FS; Fink, GR | 1 |
| Lee, GS; Ritzel, RG; Savage, EA; von Borstel, RC | 1 |
| Ronne, H; Rothstein, R | 1 |
| Boeke, JD; Fink, GR; Natsoulis, G; Trueheart, J | 1 |
| Antonova, M; Chelibonova-Lorer, H; Gavazova, E; Ivanov, S | 1 |
| Golovinsky, E; Karamanov, G; Maneva, L; Nikolova, K | 1 |
| Arenhövel, C; Golovinsky, E; Schuster, G | 1 |
| Heath, TD; Lewis, GP; Lopez, NG; Stern, WH | 1 |
| Heath, TD; Lopez, NG; Papahadjopoulos, D; Stern, WH | 1 |
| Malt, RA; Ouellette, AJ; Silverberg, MB | 1 |
| Gutteridge, WE; Hammond, DJ | 1 |
| Eriksson, G; Stenram, U | 1 |
| Boeke, JD; Fink, GR; LaCroute, F | 1 |
| Jacobs, E; Längle-Rouault, F | 1 |
| Gassis, S; Khosla, M; Lutz, C; Rathod, PK; Young, RD | 1 |
| Allshire, RC; Cranston, G; Nimmo, ER | 1 |
| Grunstein, M; Ling, X; Thompson, JS | 1 |
| Alvarez, MI; Campuzano, V; de Vicente, JI; del Valle, P; Eslava, AP | 1 |
| Akileswaran, L; Alic, M; Clark, EK; Gold, MH; Hornick, JL | 1 |
| Rathod, PK; Young, RD | 1 |
| Hekmat-Nejad, M; Rathod, PK | 1 |
| Gassis, S; Rathod, PK | 1 |
| Suzuki, T; Ueda, T; Watanabe, K | 1 |
| Grogan, DW; Jacobs, KL | 1 |
| Lee, PC; McErlean, T; Rathod, PK | 1 |
| Miceli, MV; Niesman, MR; Peyman, GA | 1 |
| Christopherson, RI; Rieckmann, KH; Seymour, KK; Yeo, AE | 1 |
| Davis, BP; Hendrick, J; Jeffries, TW; Lu, P | 1 |
| Igarashi, K; Isohara, T; Kohno, S; Shigeta, K; Uno, I; Yamano, T | 1 |
| Alvarez, MI; Eslava, AP; Iturriaga, EA; Velayos, A | 1 |
| Hisatomi, T; Kuroyanagi, S; Tsuboi, M | 1 |
| Atkinson, K; Chand-Goyal, T; Droby, S; Eckert, JW; Glickmann, E | 1 |
| Dolinski, KJ; Heitman, J | 1 |
| Goldstein, AL; McCusker, JH; Pan, X | 1 |
| Katze, MG; Park, YW; Tan, SL | 1 |
| Carbon, J; Fitch, IT; Kahana, JA; McIntosh, JR; Morphew, MK; Saunders, WS; Silver, PA; Zeng, X | 1 |
| Ishikawa, F; Matsuura, A; Naito, T | 1 |
| Kim, BG; Kwon, ST; Magae, Y; Yoo, YB | 1 |
| DasSarma, S; Krebs, MP; Peck, RF | 1 |
| Martusewitsch, E; Schleper, C; Sensen, CW | 1 |
| Kahn, ML; Mortimer, MW; Rogers, KN; Yurgel, S | 1 |
| Santoso, D; Thornburg, R | 1 |
| Grogan, DW; Reilly, MS | 1 |
| Huang, DC; Puthalakath, H; Strasser, A | 1 |
| Andersen, GR; Kinzy, TG; Nyborg, J; Pedersen, L; Valente, L | 1 |
| Richards, JD; Shaw, JM; Stillman, DJ; Voth, WP | 1 |
| Aris, JP; Wu, K; Wu, P | 1 |
| Gautsch, J; Hoekstra, D; Saghbini, M | 1 |
| Sniegowski, P | 1 |
| Jiang, YW | 1 |
| Beck, P; Dingermann, T; Winckler, T | 1 |
| Arod, F; Dubler, E; Schmalle, HW; Schneider, AG | 1 |
| Ajima, J; Maki, H; Umezu, K | 1 |
| Gou, X; Zhang, Y | 1 |
| Grogan, DW; Hansen, JE | 1 |
| Defossez, PA; Fourel, G; Gilson, E; Lebrun, E | 1 |
| CHAUDHURI, NK; HEIDELBERGER, C; MONTAG, BJ | 1 |
| HEIDELBERGER, C; WAGNER, NJ | 1 |
| BONO, VH; CHENG, CC; FREI, E; KELLY, MG | 1 |
| BASCH, RS; KARNOFSKY, DA | 1 |
| LEVIT, F | 1 |
| Gerngross, TU; Nett, JH | 1 |
| Gätgens, C; Schlösser, T; Stahmann, KP; Weber, U | 1 |
| Cho, SK; Hofmann, SL | 1 |
| Kuroiwa, T; Minoda, A; Sakagami, R; Tanaka, K; Yagisawa, F | 1 |
| Rustchenko, E; Wellington, M | 1 |
| Catalano, J; Daldal, F; Sanders, C; Yano, T | 1 |
| Davis, TN; Widlund, PO | 1 |
| Dujon, B; Fischer, G; Koszul, R | 1 |
| Kirkland, KT; Liebman, SW; Manogaran, AL | 1 |
| Giesecke, AV; Joung, JK; Meng, X; Smith, RM; Wolfe, SA | 1 |
| Akada, R; Hoshida, H; Ito, S; Kakihara, Y; Kaneko, S; Kida, K; Kitagawa, T; Kondo, A; Morimura, S; Toyonaga, D | 1 |
| Garrett, RA; Redder, P | 1 |
| Mansfield, TA; Mourad, GS; Prabhakar, JT; Schultes, NP; Snook, BM | 1 |
| Alhazzazi, T; Capone, RF; Fenno, JC; Ning, Y; Pakulis, N | 1 |
| Engelbrecher, A; Häuser, R; Titz, B; Uetz, P | 1 |
| Guss, JM; Lee, M; Maher, MJ | 1 |
| Chan, CW; Christopherson, RI; Graham, SC; Guss, JM; Lee, M; Maher, MJ | 1 |
| Bethke, L; Lakhia, R; Rangarajan, R; Thomas, S; Walker, K; Wirth, D | 1 |
| Hirai, H; Kimura, T; Oita, S; Sugiura, T; Yamagishi, K | 1 |
| Cregg, JM; Tolstorukov, I | 1 |
| Georgianna, DR; He, ZM; Obrian, GR; Payne, GA; Price, MS | 1 |
| Armstrong, J; Bone, N; Carr, AM; Garcia, V; Watson, AT | 1 |
| Ko, N; Nishihama, R; Pringle, JR | 1 |
| Baumeister, R; Dirnberger, D; Messerschmid, M | 1 |
| Acar, M; Mettetal, JT; van Oudenaarden, A | 1 |
| Hata, Y; Kondo, A; Kotaka, A; Sahara, H; Ueda, M | 1 |
| Ishih, A; Kano, S; Kino, H; Muregi, FW | 1 |
| Akada, R; Fukuda, H; Kaneko, S; Kondo, A; Noda, H; Tanaka, T | 1 |
| Beyer, AL; Jupiter, S; Lundkvist, P; Osheim, YN; Segerstolpe, A; Wieslander, L | 1 |
| Furukido, T; Jin, FJ; Koyama, Y; Nishida, M; Ogawa, M; Takahashi, T; Tokuoka, M; Utsushikawa, M | 1 |
| Afonso, B; Ajo-Franklin, CM; Silver, PA | 1 |
| Alaamery, MA; Allain, C; Ceyhan, O; Demirbas, D; Hoffman, CS; Ivey, FD; Wang, L; Wyman, AR | 1 |
| Li, YP; Wang, BH; Xu, Y | 1 |
| Argyros, DA; Barrett, TF; Caiazza, NC; Hogsett, DA; Lynd, LR; McCool, JD; Miller, BB; Murphy, DM; Olson, DG; Rajgarhia, VB; Tripathi, SA; Warner, AK | 1 |
| Fujita, Y; Itadani, A; Matsuzawa, T; Takegawa, K; Tanaka, N; Tohda, H | 1 |
| Matsuda, Y; Nakajima, K; Sakaguchi, T | 1 |
| Chabes, A; Luo, W; Rossmann, MP; Stillman, B; Tsaponina, O | 1 |
| Jerger, K; Kieler-Ferguson, HM; Riviere, K; Szoka, FC | 1 |
| Han, J; Liu, H; Liu, X; Xiang, H; Zhou, J | 1 |
| Carr, AM; Kunkel, TA; Miyabe, I | 1 |
| Linder, P; Redder, P | 1 |
| Murakami, A; Suzuki, H; Yoshida, K | 1 |
| Fairlamb, AH; Ong, HB; Patterson, S; Sienkiewicz, N; Wyllie, S | 1 |
| Cui, GZ; Cui, Q; Feng, Y; Hong, W; Liu, YJ; Xu, C; Zhang, J | 1 |
| Alcolea Palafox, M; Cuellar, A; Kiefer, W; Rastogi, VK; Rathor, SK; Schlücker, S | 1 |
| de Medeiros, AS; Hoffman, CS | 1 |
| Kurniyati, K; Li, C | 1 |
| Genee, HJ; Jensen, K; Rugbjerg, P; Sarup-Lytzen, K; Sommer, MO | 1 |
| Gong, C; Ito, T; Kawamoto, J; Kurihara, T | 1 |
| Honda, Y; Irie, T; Nakazawa, T; Sakamoto, M; Tsuzuki, M | 1 |
| Ishiwada, N; Kamei, K; Kawamoto, S; Ohkusu, M; Shimizu, K; Takahashi-Nakaguchi, A; Toh-E, A; Watanabe, A | 1 |
| Duina, AA; Turkal, CE | 1 |
| Kawaguchi, T; Kunitake, E; Sumitani, JI; Tani, S; Yuki, S | 1 |
| Kitamoto, H; Koike, H; Morita, T; Sameshima-Yamashita, Y; Shimoi, H; Watanabe, T; Yarimizu, T | 1 |
| Basen, M; Geiger, I; Henke, L; Müller, V | 1 |
| Kumakura, N; Shirasu, K; Ueno, A | 1 |
| Chen, R; Li, Z; Ma, X; Song, Q; Xiao, X; Xu, J | 1 |
| Bruni, GO; Lee, SC; Wang, P; Zhong, K | 1 |
| Billmyre, RB; Chang, Z; Heitman, J; Lee, SC | 1 |
| Bao, G; Chanda, MK; Lee, CM; Martin, R; Patterson, JO; Porteus, MH; Srifa, W; Wiebking, V | 1 |
| Fan, K; Li, J; Li, Y; Li, Z; Liu, W; Liu, Y; Pang, S; Qiu, S; Sun, Q; Wang, W; Wang, X; Wang, Y; Yang, W; Yang, Y; Yin, H; Yin, S; Yuan, F; Zhang, Q | 1 |
1 review(s) available for 5-fluoroorotic acid and orotic acid
| Article | Year |
|---|---|
Evolution: constantly avoiding mutation.
Topics: Archaeal Proteins; Bacterial Proteins; Evolution, Molecular; Genome, Archaeal; Mutation; Orotic Acid; Sulfolobus acidocaldarius | 2001 |
136 other study(ies) available for 5-fluoroorotic acid and orotic acid
| Article | Year |
|---|---|
Novel insights for dihydroorotate dehydrogenase class 1A inhibitors discovery.
Topics: Amino Acid Sequence; Biocatalysis; Computational Biology; Crystallography, X-Ray; Dihydroorotate Dehydrogenase; Dose-Response Relationship, Drug; Drug Discovery; Enzyme Inhibitors; Humans; Leishmania major; Ligands; Models, Molecular; Molecular Sequence Data; Molecular Structure; Oxidoreductases Acting on CH-CH Group Donors; Protein Structure, Tertiary; Sequence Alignment; Stereoisomerism; Structure-Activity Relationship; Trypanosoma cruzi | 2010 |
Resistance to 5-fluoroorotic acid and pyrimidine auxotrophy: a new bidirectional selective system for mammalian cells.
Topics: Animals; Clone Cells; Culture Media; Drug Resistance; Leukemia, Experimental; Mice; Orotic Acid; Phenotype; Uridine | 1979 |
Antimalarial activity of orotate analogs that inhibit dihydroorotase and dihydroorotate dehydrogenase.
Topics: Animals; Antimalarials; Chloroquine; Dihydroorotase; Dihydroorotate Dehydrogenase; Kinetics; Macrophages; Malaria; Mice; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Plasmodium berghei; Plasmodium falciparum; Pyrimidines | 1992 |
Molecular targets of 5-fluoroorotate in the human malaria parasite, Plasmodium falciparum.
Topics: Animals; Carbon Radioisotopes; Cells, Cultured; Erythrocytes; Humans; Orotic Acid; Plasmodium falciparum; Tetrahydrofolate Dehydrogenase; Thymidylate Synthase; Tritium | 1992 |
Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium.
Topics: Animals; Blotting, Southern; Cryptococcus neoformans; Culture Media; Deoxyribonuclease EcoRI; Deoxyribonucleases, Type II Site-Specific; DNA, Fungal; Humans; Mutation; Nucleic Acid Hybridization; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Restriction Mapping; Transformation, Genetic; Uracil; Uridine | 1992 |
New shuttle vectors for direct cloning in Saccharomyces cerevisiae.
Topics: Base Sequence; Cloning, Molecular; Genes, Fungal; Genetic Vectors; Molecular Sequence Data; Orotic Acid; Plasmids; Restriction Mapping; Retroviridae; Saccharomyces cerevisiae | 1991 |
Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.
Topics: Ampicillin; Ampicillin Resistance; Centromere; Electrophoresis, Agar Gel; Ethyl Methanesulfonate; Mutation; Orotic Acid; Plasmids; Saccharomyces; Saccharomyces cerevisiae; Transformation, Genetic; Uracil | 1991 |
Positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid.
Topics: Culture Media; Genotype; Mutation; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Species Specificity; Sulfolobus acidocaldarius; Uracil | 1991 |
Plasmodium yoelii: oral delivery of 5-fluoroorotate to treat malaria in mice.
Topics: Administration, Oral; Animals; Antimalarials; Injections, Intraperitoneal; Malaria; Mice; Orotic Acid; Plasmodium yoelii | 1991 |
Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.
Topics: Blotting, Southern; Dictyostelium; Drug Resistance, Microbial; Genes, Fungal; Genotype; Kinetics; Multienzyme Complexes; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Phenotype; Plasmids; Restriction Mapping; Transformation, Genetic | 1991 |
The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.
Topics: Blotting, Southern; Chromatography, Thin Layer; Drug Resistance, Microbial; Genes, Fungal; Mutation; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Transformation, Genetic; Trichoderma; Uridine | 1990 |
Ocular cicatricial disease. Drug effects in vitro on cell proliferation, contraction, and viability.
Topics: Animals; Cell Division; Cell Survival; Cells, Cultured; Cicatrix; Dactinomycin; Daunorubicin; Orotic Acid; Rabbits; Retinal Diseases; Skin; Tunicamycin | 1990 |
Antimalarial activity of a combination of 5-fluoroorotate and uridine in mice.
Topics: Animals; Drug Combinations; Malaria; Male; Mice; Orotic Acid; Plasmodium yoelii; Uridine | 1990 |
The use of a liposome-encapsulated 5-fluoroorotate for glaucoma surgery: I. Animal studies.
Topics: Animals; Ciliary Body; Conjunctiva; Disease Models, Animal; Drug Carriers; Glaucoma; Intraocular Pressure; Iris; Liposomes; Macaca fascicularis; Orotic Acid; Surgical Flaps; Wound Healing | 1989 |
Selective activity of 5-fluoroorotic acid against Plasmodium falciparum in vitro.
Topics: Animals; Antimalarials; Cell Survival; Cells, Cultured; Chloroquine; Humans; Orotic Acid; Plasmodium falciparum; Tumor Cells, Cultured; Uridine | 1989 |
Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II.
Topics: Blotting, Southern; Deoxyribonuclease EcoRI; DNA Topoisomerases, Type I; DNA Topoisomerases, Type II; DNA, Fungal; DNA, Ribosomal; Genes, Fungal; Genotype; Meiosis; Mitosis; Multigene Family; Mutation; Orotic Acid; Plasmids; Polymorphism, Restriction Fragment Length; Recombination, Genetic; Saccharomyces cerevisiae; Sister Chromatid Exchange | 1988 |
The base-alteration spectrum of spontaneous and ultraviolet radiation-induced forward mutations in the URA3 locus of Saccharomyces cerevisiae.
Topics: Adenine; Base Sequence; Cyclobutanes; DNA Mutational Analysis; DNA, Fungal; Genes, Fungal; Genetic Vectors; Orotic Acid; Plasmids; Pyrimidine Dimers; Saccharomyces cerevisiae; Ultraviolet Rays | 1988 |
Mitotic sectored colonies: evidence of heteroduplex DNA formation during direct repeat recombination.
Topics: Canavanine; Culture Media; DNA Repair; DNA, Fungal; Genes, Fungal; Mitosis; Orotic Acid; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; RNA, Transfer; Saccharomyces cerevisiae; Selection, Genetic; Suppression, Genetic | 1988 |
5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
Topics: Alleles; Genes, Fungal; Genetic Engineering; Mutation; Orotic Acid; Plasmids; Saccharomyces cerevisiae | 1987 |
Effect of fluororotic acid on the sialylation of the chicken liver, hepatoma MC-29 and serum glycoconjugates.
Topics: Animals; Carbon Radioisotopes; Chickens; Glucosamine; Glycoconjugates; Hexosamines; Liver; Liver Neoplasms, Experimental; Orotic Acid; Sialic Acids | 1988 |
Studies on the antimycotic activity of 5-fluoroorotic acid.
Topics: Adolescent; Adult; Aged; Animals; Antifungal Agents; Arthrodermataceae; Candida; Dermatomycoses; Female; Flucytosine; Humans; Liniments; Male; Mice; Microbial Sensitivity Tests; Middle Aged; Ointments; Orotic Acid; Powders | 1987 |
Analogues of pyrimidine base precursors as antiphytoviral agents.
Topics: Antiviral Agents; Cycloheximide; Fluorouracil; Orotic Acid; Plant Viruses; Thiouracil; Triazines; Virus Replication | 1987 |
Antiproliferative and anticontractile effects of liposome encapsulated fluoroorotate.
Topics: Animals; Eye Diseases; Fibroblasts; Liposomes; Orotic Acid; Rabbits; Retinal Diseases; Vitreous Body | 1987 |
5-Fluoroorotate: a new liposome-dependent cytotoxic agent.
Topics: Animals; Antineoplastic Agents; Cell Division; Cell Line; Cell Survival; Liposomes; Neoplasms, Experimental; Orotic Acid; Time Factors | 1985 |
Association of poly(adenylate)-deficient messenger ribonucleic acid with membranes in mouse kidney.
Topics: Animals; Cell Membrane; Kidney; Male; Mice; Orotic Acid; Poly A; Polyribosomes; RNA, Messenger; RNA, Ribosomal | 1981 |
Studies on the glycosomal orotate phosphoribosyl transferase of Trypanosoma cruzi.
Topics: Animals; Chagas Disease; Hydrogen-Ion Concentration; Magnesium; Microbodies; Organoids; Orotate Phosphoribosyltransferase; Orotic Acid; Pentosyltransferases; Phosphoribosyl Pyrophosphate; Substrate Specificity; Trypanosoma cruzi | 1983 |
The effect of 5-fluoroorotic acid on the early labelling of nucleotides and RNA in whole liver and of RNA in subnuclear fractions in rats given 3H-orotic acid.
Topics: Animals; Cell Nucleus; Liver; Orotic Acid; Rats; Ribonucleotides; RNA; Transcription, Genetic | 1984 |
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
Topics: Carboxy-Lyases; Drug Resistance, Microbial; Fungal Proteins; Microbiological Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae | 1984 |
A method for performing precise alterations in the yeast genome using a recycable selectable marker.
Topics: Base Sequence; Carrier Proteins; DNA-Binding Proteins; Fungal Proteins; Genetic Markers; Genome, Fungal; Kluyveromyces; Molecular Sequence Data; Mutagenesis, Site-Directed; MutL Proteins; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sequence Deletion; Transcription Factors | 1995 |
Selection and characterization of 5-fluoroorotate-resistant Plasmodium falciparum.
Topics: Animals; Drug Resistance; Fluorouracil; Orotic Acid; Plasmodium falciparum; Pyrimidines; Ultraviolet Rays | 1994 |
Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes.
Topics: Adenine; Cell Division; Chromosome Aberrations; DNA, Recombinant; Electrophoresis, Gel, Pulsed-Field; Extrachromosomal Inheritance; Gene Expression Regulation, Fungal; Gene Rearrangement; Genes, Fungal; Leucine; Orotic Acid; RNA, Fungal; Schizosaccharomyces; Uracil | 1994 |
Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast.
Topics: Amino Acid Sequence; Chromosomes, Fungal; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Fungal; Histones; Lysine; Molecular Sequence Data; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae; Telomere | 1994 |
Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.
Topics: Cell-Free System; Drug Resistance, Microbial; Genetic Complementation Test; Genetic Linkage; Genetic Markers; Genotype; Orotate Phosphoribosyltransferase; Orotic Acid; Phycomyces; Pyrimidines; Recombination, Genetic; Restriction Mapping; Spores, Fungal | 1993 |
Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Topics: Ascomycota; Basidiomycota; Drug Resistance, Microbial; Genes, Fungal; Lignin; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Recombination, Genetic; Transformation, Genetic; Uracil | 1993 |
Clonal viability measurements on Plasmodium falciparum to assess in vitro schizonticidal activity of leupeptin, chloroquine, and 5-fluoroorotate.
Topics: Animals; Antimalarials; Cell Division; Chloroquine; Drug Resistance; Humans; Leupeptins; Orotic Acid; Plasmodium falciparum | 1993 |
Kinetics of Plasmodium falciparum thymidylate synthase: interactions with high-affinity metabolites of 5-fluoroorotate and D1694.
Topics: Animals; Antimalarials; Enzyme Inhibitors; Fluorodeoxyuridylate; Kinetics; Orotic Acid; Plasmodium falciparum; Quinazolines; Thiophenes; Thymidylate Synthase | 1996 |
Frequency of drug resistance in Plasmodium falciparum: a nonsynergistic combination of 5-fluoroorotate and atovaquone suppresses in vitro resistance.
Topics: Animals; Antimalarials; Atovaquone; Cell Line; Dose-Response Relationship, Drug; Drug Interactions; Drug Resistance, Microbial; Mice; Naphthoquinones; Orotic Acid; Plasmodium falciparum | 1996 |
The 'polysemous' codon--a codon with multiple amino acid assignment caused by dual specificity of tRNA identity.
Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Anticodon; Base Sequence; Candida; Chromatography, Thin Layer; Codon; Electrophoresis, Polyacrylamide Gel; Genetic Complementation Test; Kinetics; Leucine; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Orotic Acid; Protein Biosynthesis; Ribonucleases; RNA, Transfer, Amino Acyl; RNA, Transfer, Leu; Serine | 1997 |
Rates of spontaneous mutation in an archaeon from geothermal environments.
Topics: DNA Damage; Hot Temperature; Mutation; Orotic Acid; Sulfolobus acidocaldarius | 1997 |
Variations in frequencies of drug resistance in Plasmodium falciparum.
Topics: Animals; Antimalarials; Atovaquone; Drug Resistance; Gene Frequency; Naphthoquinones; Orotic Acid; Plasmodium falciparum | 1997 |
Liposome uptake by human retinal pigment epithelial cells in culture.
Topics: Adult; Aged; Aged, 80 and over; Animals; Arylsulfonates; Cell Survival; Cells, Cultured; Coloring Agents; Fibroblasts; Fluorescent Dyes; Humans; Liposomes; Macrophages; Microscopy, Fluorescence; Middle Aged; Orotic Acid; Phagocytosis; Phospholipids; Pigment Epithelium of Eye; Rabbits; Tetrazolium Salts; Thiazoles | 1997 |
dCTP levels are maintained in Plasmodium falciparum subjected to pyrimidine deficiency or excess.
Topics: Animals; Antimalarials; Atovaquone; Deoxycytosine Nucleotides; Dihydroorotase; Dihydroorotate Dehydrogenase; Naphthoquinones; Nucleotides; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Plasmodium falciparum; Pyrimidines | 1997 |
Cloning and disruption of the beta-isopropylmalate dehydrogenase gene (LEU2) of Pichia stipitis with URA3 and recovery of the double auxotroph.
Topics: 3-Isopropylmalate Dehydrogenase; Alcohol Oxidoreductases; Blotting, Southern; Cloning, Molecular; DNA, Fungal; Drug Resistance, Microbial; Fungal Proteins; Genes, Fungal; Genetic Vectors; Mutagenesis, Insertional; Orotic Acid; Pichia; Polymerase Chain Reaction; Sequence Analysis, DNA; Transformation, Genetic | 1998 |
A novel indication of the activities of small molecule inhibitors of protein-protein interactions: application of the yeast two-hybrid system.
Topics: Cell Division; Cycloheximide; Drug Evaluation, Preclinical; Enzyme Inhibitors; Histidine; Hybrid Cells; Immunophilins; Orotic Acid; Protein Synthesis Inhibitors; Receptors, Transforming Growth Factor beta; Tacrolimus; Tacrolimus Binding Proteins; Yeasts | 1998 |
Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides.
Topics: Alleles; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Dimerization; Fungal Proteins; Genetic Complementation Test; Models, Molecular; Molecular Sequence Data; Mucor; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Protein Conformation; Pyrimidines; Sequence Analysis; Transformation, Genetic | 1998 |
Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3.
Topics: Genes, Fungal; Genetic Vectors; Mutation; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Plasmids; Saccharomyces; Transformation, Genetic; Uracil | 1998 |
Transformation of Candida oleophila and survival of a transformant on orange fruit under field conditions.
Topics: 2-Aminoadipic Acid; Candida; Citrus; Cloning, Molecular; DNA, Fungal; Drug Resistance; Genes, Fungal; Genetic Markers; Glucuronidase; Histidine; Mutation; Orotic Acid; Pest Control, Biological; Plant Diseases; Plasmids; Saccharomyces cerevisiae; Transformation, Genetic | 1999 |
Hmo1p, a high mobility group 1/2 homolog, genetically and physically interacts with the yeast FKBP12 prolyl isomerase.
Topics: Alleles; beta-Galactosidase; Blotting, Western; Dimerization; Fungal Proteins; Genetic Testing; Glucose; High Mobility Group Proteins; Immunophilins; Leucine; Models, Biological; Mutagenesis; Orotic Acid; Phenotype; Receptors, Formyl Peptide; Receptors, Immunologic; Receptors, Peptide; Saccharomyces cerevisiae Proteins; Sirolimus; Tacrolimus; Tacrolimus Binding Proteins; Transformation, Genetic; Yeasts | 1999 |
Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae.
Topics: Candida albicans; Deoxyribonucleases, Type II Site-Specific; Drug Resistance, Microbial; Fungal Proteins; Genes, Fungal; Genetic Markers; Kanamycin; Mutagenesis, Insertional; Open Reading Frames; Orotic Acid; Plasmids; Recombination, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Selection, Genetic; Sequence Homology, Nucleic Acid | 1999 |
Differential sensitivity to 5-fluoro-orotic acid as a screen for bait RNA-independent false positives in a yeast three-hybrid system.
Topics: Cloning, Molecular; DNA, Complementary; DNA, Fungal; False Positive Reactions; Gene Library; Genetic Testing; Orotic Acid; RNA, Fungal; RNA, Messenger; Sensitivity and Specificity; Yeasts | 1999 |
Slk19p is a centromere protein that functions to stabilize mitotic spindles.
Topics: Anaphase; Cell Nucleus; Centromere; Cytoplasm; Fungal Proteins; Genes, Lethal; Kinesins; Kinetochores; Microscopy, Fluorescence; Microtubule-Associated Proteins; Microtubules; Mitosis; Mutation; Orotic Acid; Phenotype; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spindle Apparatus | 1999 |
Genetic control of telomere integrity in Schizosaccharomyces pombe: rad3(+) and tel1(+) are parts of two regulatory networks independent of the downstream protein kinases chk1(+) and cds1(+).
Topics: Adenosine Triphosphatases; Ataxia Telangiectasia Mutated Proteins; Cell Cycle Proteins; Checkpoint Kinase 1; Checkpoint Kinase 2; Chromosomes, Fungal; DNA Helicases; DNA-Binding Proteins; Fungal Proteins; Orotic Acid; Protein Kinases; Protein Serine-Threonine Kinases; Proteins; Radiation Tolerance; Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Signal Transduction; Telomere; Tumor Suppressor Proteins | 1999 |
Isolation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus.
Topics: Aspartate Carbamoyltransferase; Blotting, Southern; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); DNA, Fungal; Drug Resistance, Microbial; Fungal Proteins; Genetic Vectors; Multienzyme Complexes; Mutation; Orotic Acid; Plasmids; Pleurotus; Saccharomyces cerevisiae Proteins; Transformation, Genetic; Trichoderma; Ultraviolet Rays; Uracil | 1999 |
Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker.
Topics: Amino Acid Sequence; Archaeal Proteins; Cloning, Molecular; Fungal Proteins; Genetic Complementation Test; Genetic Engineering; Genetic Markers; Halobacterium salinarum; Molecular Sequence Data; Mutation; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Selection, Genetic; Sequence Homology, Amino Acid | 2000 |
High spontaneous mutation rate in the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by transposable elements.
Topics: Amino Acid Sequence; Base Sequence; DNA Transposable Elements; DNA, Archaeal; Genome, Archaeal; Molecular Sequence Data; Mutagenesis, Insertional; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Sequence Homology, Amino Acid; Sulfolobus; Uracil | 2000 |
New substrates for the dicarboxylate transport system of Sinorhizobium meliloti.
Topics: Bacterial Proteins; Biological Transport, Active; Carrier Proteins; Dicarboxylic Acid Transporters; Dicarboxylic Acids; Escherichia coli; Kinetics; Orotic Acid; Sinorhizobium meliloti; Succinic Acid | 2000 |
Fluoroorotic acid-selected Nicotiana plumbaginifolia cell lines with a stable thymine starvation phenotype have lost the thymine-regulated transcriptional program.
Topics: Aspartate Carbamoyltransferase; Blotting, Western; Cell Line; Chromatography, Thin Layer; Dihydroorotase; Dihydroorotate Dehydrogenase; Gene Expression Regulation, Plant; Multienzyme Complexes; Nicotiana; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Phenotype; Plants, Toxic; Pyrimidines; RNA, Messenger; Thymine | 2000 |
Characterization of intragenic recombination in a hyperthermophilic archaeon via conjugational DNA exchange.
Topics: Alleles; Archaeal Proteins; Bacterial Proteins; Conjugation, Genetic; DNA-Binding Proteins; Drug Resistance, Microbial; Frameshift Mutation; Genes, Archaeal; Integration Host Factors; Orotic Acid; Promoter Regions, Genetic; Pseudomonas; Recombination, Genetic; Sulfolobus acidocaldarius; Temperature; Trans-Activators; Transcription Factors | 2001 |
Rapid selection against truncation mutants in yeast reverse two-hybrid screens.
Topics: Apoptosis Regulatory Proteins; Bcl-2-Like Protein 11; beta-Galactosidase; Binding Sites; Carrier Proteins; Drosophila Proteins; Dyneins; Flow Cytometry; Fluorouracil; Green Fluorescent Proteins; Luminescent Proteins; Membrane Proteins; Mutagenesis; Mutation, Missense; Orotic Acid; Point Mutation; Polymerase Chain Reaction; Proto-Oncogene Proteins; Recombinant Fusion Proteins; Restriction Mapping; Saccharomyces cerevisiae; Transfection | 2001 |
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex.
Topics: Amino Acid Substitution; Binding Sites; Carbohydrate Metabolism; Crystallography, X-Ray; Guanosine Diphosphate; Lysine; Magnesium; Models, Molecular; Mutation; Orotic Acid; Peptide Elongation Factor 1; Protein Conformation; Yeasts | 2001 |
Yeast vectors for integration at the HO locus.
Topics: Ampicillin Resistance; Deoxyribonucleases, Type II Site-Specific; DNA, Bacterial; DNA, Fungal; Drug Resistance, Microbial; Escherichia coli; Fungal Proteins; Genes, Fungal; Genetic Markers; Genetic Vectors; Gentamicins; Kanamycin Resistance; Molecular Sequence Data; Orotic Acid; Recombination, Genetic; Restriction Mapping; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Tandem Repeat Sequences | 2001 |
Nucleolar protein Nop12p participates in synthesis of 25S rRNA in Saccharomyces cerevisiae.
Topics: Alleles; Amino Acid Sequence; Cell Division; Cold Temperature; Fungal Proteins; Gene Deletion; Genetic Complementation Test; Molecular Sequence Data; Mutation; Nuclear Proteins; Orotic Acid; Phenotype; RNA Precursors; RNA Processing, Post-Transcriptional; RNA, Ribosomal; Saccharomyces cerevisiae; Sequence Homology, Amino Acid | 2001 |
Media formulations for various two-hybrid systems.
Topics: Adenine; Carbon; Culture Media; Cycloheximide; Fungal Proteins; Genes, Reporter; Genetic Techniques; Genetic Vectors; Mycology; Orotic Acid; Protein Processing, Post-Translational; RNA, Fungal; Saccharomyces cerevisiae; Two-Hybrid System Techniques | 2001 |
Transcriptional cosuppression of yeast Ty1 retrotransposons.
Topics: Base Sequence; Chromatin; Chromosomes, Fungal; DNA Methylation; Feedback; Fungal Proteins; Gene Expression Regulation, Fungal; Gene Silencing; Genes, Reporter; Molecular Sequence Data; Orotic Acid; Promoter Regions, Genetic; Recombinant Fusion Proteins; Retroelements; Saccharomyces cerevisiae; Selection, Genetic; Sequence Homology, Nucleic Acid; Terminal Repeat Sequences; Transcription, Genetic | 2002 |
Transfer RNA gene-targeted retrotransposition of Dictyostelium TRE5-A into a chromosomal UMP synthase gene trap.
Topics: Animals; Base Sequence; Dictyostelium; DNA, Protozoan; Gene Targeting; Genes, Protozoan; Genes, Reporter; Molecular Sequence Data; Multienzyme Complexes; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Phenotype; Retroelements; RNA, Protozoan; RNA, Transfer | 2002 |
The interaction of 5-fluoroorotic acid with transition metals: synthesis and characterisation of Ni(II), Cu(II) and Zn(II) complexes.
Topics: Antimalarials; Copper; Crystallography, X-Ray; Models, Molecular; Molecular Structure; Nickel; Orotic Acid; Spectrophotometry, Infrared; Thermogravimetry; Zinc | 2002 |
Elevated incidence of loss of heterozygosity (LOH) in an sgs1 mutant of Saccharomyces cerevisiae: roles of yeast RecQ helicase in suppression of aneuploidy, interchromosomal rearrangement, and the simultaneous incidence of both events during mitotic growt
Topics: Adenosine Triphosphatases; Aneuploidy; Cell Division; Chromosome Aberrations; DNA Helicases; DNA, Fungal; Drug Resistance, Microbial; Gene Frequency; Genotype; Loss of Heterozygosity; Mitosis; Mutation; Orotic Acid; Phenotype; Plasmids; RecQ Helicases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 2002 |
[Characterization and transformation of anti-FOA strain of Aspergillus awamori].
Topics: Aspergillus; Culture Media; Drug Resistance, Microbial; Fungal Proteins; Mutation; Orotic Acid; Spores, Fungal; Transformation, Genetic; Ultraviolet Rays; Uridine | 2000 |
Molecular characteristics of spontaneous deletions in the hyperthermophilic archaeon Sulfolobus acidocaldarius.
Topics: Base Sequence; Consensus Sequence; DNA, Archaeal; Evolution, Molecular; Gene Deletion; Hot Temperature; Molecular Sequence Data; Orotate Phosphoribosyltransferase; Orotic Acid; Pyrimidines; Selection, Genetic; Sequence Analysis, DNA; Sulfolobus acidocaldarius | 2003 |
A methyltransferase targeting assay reveals silencer-telomere interactions in budding yeast.
Topics: Cell Nucleus; Chromosomes; Cytoplasm; Gene Silencing; Genetic Vectors; Methyltransferases; Models, Genetic; Mutation; Orotic Acid; Plasmids; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomycetales; Site-Specific DNA-Methyltransferase (Adenine-Specific); Telomere | 2003 |
Studies on fluorinated pyrimidines. III. The metabolism of 5-fluorouracil-2-C14 and 5-fluoroorotic-2-C14 acid in vivo.
Topics: Antineoplastic Agents; Fluorides; Fluorouracil; Humans; Neoplasms; Orotic Acid; Pyrimidines | 1958 |
Some effects of 5-fluoroorotic acid and 5-fluorouracii on the soluble acid of rat liver.
Topics: Fluorouracil; Liver; Orotic Acid; Pyrimidines; RNA | 1962 |
MEHTYL-5-FLUOROOROTATE: SYNTHESIS AND COMPARISON WITH 5-FLUOROOROTIC ACID WITH RESPECT TO BIOLOGICAL ACTIVITY AND CELL ENTRY.
Topics: Animals; Leukemia L1210; Leukocytes; Metabolism; Mice; Orotic Acid; Research | 1964 |
Effects of 5-fluorodeoxyuridine and related halogenated pyrimidines on the sand-dollar embryo.
Topics: Cytidine; DNA; Female; Floxuridine; Fluorouracil; Humans; Orotic Acid; Pregnancy; Pyrimidines; Thymidine; Thymine; Uridine | 1960 |
Some effects of the intradermal injection of 5-fluoroorotic acid.
Topics: Injections, Intradermal; Orotic Acid; Skin | 1960 |
Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris.
Topics: Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Fungal; Drug Resistance, Fungal; Fungal Proteins; Genetic Vectors; Molecular Sequence Data; Mutagenesis, Insertional; Orotate Phosphoribosyltransferase; Orotic Acid; Pichia; Plasmids; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Transformation, Genetic | 2003 |
Alanine : glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance.
Topics: Alanine; Amino Acid Sequence; Animals; Base Sequence; DNA, Fungal; Glycine; Glyoxylates; Humans; Models, Chemical; Molecular Sequence Data; Mutagenesis, Insertional; Orotic Acid; Saccharomyces cerevisiae; Selection, Genetic; Sequence Alignment; Transaminases | 2004 |
pdf1, a palmitoyl protein thioesterase 1 Ortholog in Schizosaccharomyces pombe: a yeast model of infantile Batten disease.
Topics: Amino Acid Sequence; Cloning, Molecular; Gene Deletion; Genetic Complementation Test; Humans; Hydrogen-Ion Concentration; Models, Genetic; Molecular Sequence Data; Neuronal Ceroid-Lipofuscinoses; Orotic Acid; Point Mutation; Protein Processing, Post-Translational; Protein Structure, Tertiary; Schizosaccharomyces; Sequence Alignment; Thiolester Hydrolases; Vanadates | 2004 |
Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D.
Topics: Amino Acid Sequence; Base Sequence; Cell Culture Techniques; Cell Nucleus; Cells, Cultured; Culture Media; DNA, Plant; Molecular Sequence Data; Multienzyme Complexes; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Recombination, Genetic; Rhodophyta; Transformation, Genetic | 2004 |
5-Fluoro-orotic acid induces chromosome alterations in Candida albicans.
Topics: Candida albicans; Chromosome Aberrations; Chromosomes, Fungal; Karyotyping; Mutation; Orotic Acid; Phenotype | 2005 |
sacB-5-Fluoroorotic acid-pyrE-based bidirectional selection for integration of unmarked alleles into the chromosome of Rhodobacter capsulatus.
Topics: Alleles; ATP-Binding Cassette Transporters; Bacterial Proteins; Base Sequence; Chromosomes, Bacterial; Hexosyltransferases; Molecular Sequence Data; Orotate Phosphoribosyltransferase; Orotic Acid; Recombination, Genetic; Rhodobacter capsulatus | 2005 |
A high-efficiency method to replace essential genes with mutant alleles in yeast.
Topics: Alleles; Fungal Proteins; Gene Targeting; Genes, Essential; Genes, Fungal; Hydroxymethyl and Formyl Transferases; Mutagenesis, Site-Directed; Mutation; Orotic Acid; Phosphoribosylglycinamide Formyltransferase; Plasmids; Temperature; Yeasts | 2005 |
Stability of large segmental duplications in the yeast genome.
Topics: Fungal Proteins; Gene Duplication; Genome, Fungal; Karyotyping; Meiosis; Mitosis; Models, Biological; Models, Genetic; Mutation; Nucleic Acid Hybridization; Orotic Acid; Saccharomyces cerevisiae; Telomere; Translocation, Genetic | 2006 |
An engineered nonsense URA3 allele provides a versatile system to detect the presence, absence and appearance of the [PSI+] prion in Saccharomyces cerevisiae.
Topics: Alleles; Codon, Nonsense; DNA, Fungal; Fungal Proteins; Mutagenesis, Insertional; Orotic Acid; Peptide Termination Factors; Prions; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transformation, Genetic | 2006 |
Counter-selectable marker for bacterial-based interaction trap systems.
Topics: Escherichia coli; Gene Deletion; Gene Expression; Genes, Bacterial; Genes, Reporter; Genetic Markers; Genetic Vectors; Mutagenesis, Insertional; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Plasmids; Recombination, Genetic; Selection, Genetic; Sensitivity and Specificity; Transformation, Bacterial; Two-Hybrid System Techniques | 2006 |
PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae.
Topics: DNA, Fungal; Fungal Proteins; Gene Deletion; Genetic Markers; Mutagenesis, Insertional; Orotic Acid; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae | 2006 |
Mutations and rearrangements in the genome of Sulfolobus solfataricus P2.
Topics: Archaeal Proteins; DNA Mutational Analysis; DNA Transposable Elements; Drug Resistance, Microbial; Gene Rearrangement; Genome, Archaeal; Mutation; Orotic Acid; Sulfolobus solfataricus | 2006 |
A fluoroorotic acid-resistant mutant of Arabidopsis defective in the uptake of uracil.
Topics: Arabidopsis; Arabidopsis Proteins; Biological Transport; Chromosome Mapping; Chromosomes, Plant; Escherichia coli; Escherichia coli Proteins; Genetic Complementation Test; Membrane Transport Proteins; Mutation; Orotic Acid; Uracil | 2006 |
Characterization of Treponema denticola pyrF encoding orotidine-5'-monophosphate decarboxylase.
Topics: Bacterial Proteins; Culture Media; Drug Resistance, Bacterial; Genetic Complementation Test; Mutation; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Pyrimidines; Treponema denticola; Uracil | 2007 |
The Escherichia coli protein YjjG is a house-cleaning nucleotidase in vivo.
Topics: Azacitidine; Bromodeoxyuridine; Decitabine; Deoxyuridine; DNA Damage; Escherichia coli; Escherichia coli Proteins; Fluorouracil; Models, Biological; Molecular Structure; Mutation; N-Glycosyl Hydrolases; Nucleotidases; Orotic Acid; Substrate Specificity; Thymidine Monophosphate; Uridine | 2007 |
Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form.
Topics: Amino Acid Substitution; Catalytic Domain; Crystallography, X-Ray; Dihydroorotase; Escherichia coli Proteins; Mutation; Orotic Acid; Serine; Structure-Activity Relationship; Substrate Specificity; Threonine | 2007 |
Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design.
Topics: Amino Acid Sequence; Binding Sites; Catalytic Domain; Dihydroorotase; Escherichia coli Proteins; Hydrogen Bonding; Ligands; Models, Molecular; Molecular Sequence Data; Movement; Orotic Acid; Protein Conformation; Zinc | 2007 |
The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites.
Topics: Animals; DNA Mismatch Repair; Drug Resistance; Genetic Variation; Microsatellite Repeats; Mutation; Orotic Acid; Parasitic Sensitivity Tests; Plasmodium berghei; Plasmodium falciparum; Polymorphism, Genetic; Protozoan Proteins; Rats | 2007 |
Transformation by complementation of a uracil auxotroph of the hyper lignin-degrading basidiomycete Phanerochaete sordida YK-624.
Topics: Gene Expression Regulation, Fungal; Genetic Complementation Test; Glyceraldehyde-3-Phosphate Dehydrogenases; Green Fluorescent Proteins; Industrial Microbiology; Lignin; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Phanerochaete; Transformation, Genetic; Uracil | 2007 |
Classical genetics.
Topics: Autotrophic Processes; Crosses, Genetic; Genes, Essential; Genetic Complementation Test; Mutagenesis; Mutation; Nitrosoguanidines; Orotic Acid; Pichia; Uracil | 2007 |
Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus.
Topics: Antifungal Agents; Aspergillus flavus; Blotting, Southern; Drug Resistance, Fungal; Genes, Fungal; Genetic Complementation Test; Mutagenesis; Mutagenesis, Insertional; Orotic Acid; Phleomycins; Polymerase Chain Reaction; Protoplasts; Spores, Fungal; Transformation, Genetic; Uracil | 2007 |
Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe.
Topics: Amino Acid Sequence; Base Sequence; Cell Cycle Proteins; DNA-Binding Proteins; Gene Targeting; Genes, Fungal; Green Fluorescent Proteins; Integrases; Molecular Sequence Data; Orotic Acid; Polymerase Chain Reaction; Recombination, Genetic; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Transglutaminases; Uracil | 2008 |
Control of 5-FOA and 5-FU resistance by Saccharomyces cerevisiae YJL055W.
Topics: Antifungal Agents; Drug Resistance, Fungal; Fluorouracil; Gene Expression Regulation, Fungal; Orotic Acid; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 2008 |
An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor.
Topics: Brain; Cloning, Molecular; Frizzled Receptors; Fungal Proteins; Gene Library; Humans; Orotic Acid; Phenotype; Plasmids; Protein Interaction Mapping; Recombinant Fusion Proteins; Ubiquitin; Yeasts | 2008 |
Stochastic switching as a survival strategy in fluctuating environments.
Topics: Adaptation, Biological; Bacterial Proteins; Cell Survival; Environment; Fluorouracil; Fungal Proteins; Galactokinase; Galactose; Luminescent Proteins; Models, Biological; Orotic Acid; Phenotype; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Stochastic Processes; Time Factors; Uracil | 2008 |
Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity.
Topics: Diploidy; Fungal Proteins; Gene Targeting; Genetic Engineering; Loss of Heterozygosity; Orotic Acid; Recombination, Genetic; Saccharomyces cerevisiae | 2009 |
Plasmodium berghei: efficacy of 5-fluoroorotate in combination with commonly used antimalarial drugs in a mouse model.
Topics: Animals; Antimalarials; Artemisinins; Artesunate; Dapsone; Drug Synergism; Drug Therapy, Combination; Malaria; Male; Mice; Mice, Inbred ICR; Orotic Acid; Parasitemia; Plasmodium berghei; Pyrimethamine | 2009 |
Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae.
Topics: beta-Glucosidase; Culture Media; Fungal Proteins; Mutagenesis, Insertional; Orotic Acid; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Selection, Genetic | 2009 |
Mrd1p is required for release of base-paired U3 snoRNA within the preribosomal complex.
Topics: Alleles; Amino Acid Sequence; Base Pairing; Fluorouracil; Gene Expression Regulation, Fungal; Genes, Dominant; Genetic Complementation Test; Molecular Sequence Data; Mutant Proteins; Mutation; Orotic Acid; Protein Structure, Tertiary; Ribonucleoproteins; Ribosomes; RNA Precursors; RNA Processing, Post-Transcriptional; RNA-Binding Proteins; RNA, Small Nucleolar; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 2009 |
A trial of minimization of chromosome 7 in Aspergillus oryzae by multiple chromosomal deletions.
Topics: Aspergillus oryzae; Chromosome Deletion; Chromosomes, Fungal; Industrial Microbiology; Mutagenesis, Insertional; Orotic Acid | 2010 |
A synthetic circuit for selectively arresting daughter cells to create aging populations.
Topics: Cell Cycle; Genetic Engineering; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae | 2010 |
New classes of PDE7 inhibitors identified by a fission yeast-based HTS.
Topics: Anti-Inflammatory Agents; Catalytic Domain; Cyclic AMP; Cyclic Nucleotide Phosphodiesterases, Type 7; Enzyme Assays; High-Throughput Screening Assays; Humans; Hydrolysis; Inhibitory Concentration 50; Lipopolysaccharides; Orotic Acid; Phosphodiesterase Inhibitors; Schizosaccharomyces; Tumor Necrosis Factor-alpha | 2010 |
Use of the pyrG gene as a food-grade selection marker in Monascus.
Topics: Aspergillus flavus; Cloning, Molecular; DNA Primers; DNA, Fungal; Fungal Proteins; Genetic Engineering; Genetic Vectors; Genetics, Microbial; Molecular Sequence Data; Monascus; Mutagenesis; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Plasmids; Point Mutation; Polymerase Chain Reaction; Selection, Genetic; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Ultraviolet Rays | 2010 |
Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant.
Topics: Bacterial Proteins; Carboxylic Acids; Clostridium thermocellum; Gene Deletion; Gene Knockout Techniques; Metabolic Networks and Pathways; Molecular Biology; Orotic Acid; Phosphate Acetyltransferase; Plasmids; Selection, Genetic | 2010 |
New insights into galactose metabolism by Schizosaccharomyces pombe: isolation and characterization of a galactose-assimilating mutant.
Topics: Culture Media; Galactose; Gene Expression Profiling; Gene Expression Regulation, Fungal; Gene Silencing; Genes, Fungal; Mutation; Orotic Acid; RNA, Fungal; Schizosaccharomyces | 2011 |
Identification of the UMP synthase gene by establishment of uracil auxotrophic mutants and the phenotypic complementation system in the marine diatom Phaeodactylum tricornutum.
Topics: Amino Acid Sequence; Biosynthetic Pathways; Diatoms; Genetic Complementation Test; Humans; Molecular Sequence Data; Multienzyme Complexes; Mutagenesis; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Phenotype; Phylogeny; Protein Structure, Tertiary; RNA Interference; Sequence Alignment; Uracil | 2011 |
A common telomeric gene silencing assay is affected by nucleotide metabolism.
Topics: Antigens, Nuclear; Chromosomal Position Effects; Gene Silencing; Genes, Fungal; Histone-Lysine N-Methyltransferase; Models, Genetic; Mutation; Nuclear Proteins; Orotic Acid; Proliferating Cell Nuclear Antigen; Ribonucleotide Reductases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Telomere | 2011 |
Anti-tumor activity of liposome encapsulated fluoroorotic acid as a single agent and in combination with liposome irinotecan.
Topics: Animals; Antineoplastic Agents; Camptothecin; Cell Survival; Colonic Neoplasms; Drug Combinations; Drug Compounding; Drug Synergism; HT29 Cells; Humans; Irinotecan; Liposomes; Maximum Tolerated Dose; Mice; Mice, Inbred BALB C; Mice, Nude; Orotic Acid; Particle Size; Survival Analysis; Treatment Outcome; Xenograft Model Antitumor Assays | 2011 |
Development of pyrF-based gene knockout systems for genome-wide manipulation of the archaea Haloferax mediterranei and Haloarcula hispanica.
Topics: Alkyl and Aryl Transferases; Amino Acid Sequence; Archaeal Proteins; Culture Media; Gene Knockout Techniques; Genetic Vectors; Geranylgeranyl-Diphosphate Geranylgeranyltransferase; Haloarcula; Haloferax mediterranei; Molecular Sequence Data; Orotic Acid; Plasmids; Recombination, Genetic; Sequence Deletion; Uracil | 2011 |
The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.
Topics: Base Sequence; Cdc20 Proteins; Cell Cycle Proteins; DNA Replication; Evolution, Molecular; Genome, Fungal; Molecular Sequence Data; Orotic Acid; Point Mutation; Replication Origin; Ribonuclease H; Schizosaccharomyces; Schizosaccharomyces pombe Proteins | 2011 |
New range of vectors with a stringent 5-fluoroorotic acid-based counterselection system for generating mutants by allelic replacement in Staphylococcus aureus.
Topics: Alleles; Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Bacterial; Genetic Engineering; Genetic Vectors; Humans; Mutation; Orotic Acid; Recombination, Genetic; Selection, Genetic; Staphylococcus aureus | 2012 |
Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.
Topics: Bacterial Proteins; Drug Resistance, Bacterial; Gene Knockout Techniques; Genetics, Microbial; Geobacillus; Molecular Biology; Orotic Acid; Recombination, Genetic; Selection, Genetic; Uracil | 2012 |
Trypanosoma brucei (UMP synthase null mutants) are avirulent in mice, but recover virulence upon prolonged culture in vitro while retaining pyrimidine auxotrophy.
Topics: Amides; Animals; Biological Transport; Cell Line; Deoxyuridine; Fluorouracil; Gene Knockout Techniques; Mice; Multienzyme Complexes; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Pyrazoles; Pyrimidines; Ribonucleosides; Ribose; Transfection; Trypanocidal Agents; Trypanosoma brucei brucei; Uracil; Uridine; Uridine Monophosphate; Virulence | 2013 |
Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system.
Topics: Clostridium cellulolyticum; Gene Knockout Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Plasmids; Selection, Genetic | 2014 |
FT-IR and FT-Raman spectra of 5-fluoroorotic acid with solid state simulation by DFT methods.
Topics: Computer Simulation; Dimerization; Isomerism; Models, Molecular; Molecular Conformation; Orotic Acid; Quantum Theory; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Thermodynamics; Vibration | 2014 |
A yeast-based high-throughput screen for modulators of phosphodiesterase activity.
Topics: Culture Media; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Genes, Reporter; High-Throughput Screening Assays; Humans; Orotic Acid; Phosphodiesterase Inhibitors; Phosphoric Diester Hydrolases; Schizosaccharomyces | 2015 |
pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.
Topics: Drug Resistance, Bacterial; Gene Knockout Techniques; Genetics, Microbial; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Selection, Genetic; Treponema denticola | 2016 |
Molecular Buffers Permit Sensitivity Tuning and Inversion of Riboswitch Signals.
Topics: DNA; Genetic Engineering; Green Fluorescent Proteins; Models, Theoretical; Orotic Acid; Protein Domains; Riboswitch; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction | 2016 |
Development of a versatile method for targeted gene deletion and insertion by using the pyrF gene in the psychrotrophic bacterium, Shewanella livingstonensis Ac10.
Topics: Adaptation, Biological; Base Sequence; Cold Temperature; Gene Deletion; Gene Targeting; Genes, Bacterial; Mutagenesis, Insertional; Mutagenesis, Site-Directed; Organisms, Genetically Modified; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Shewanella | 2016 |
Marker recycling via 5-fluoroorotic acid and 5-fluorocytosine counter-selection in the white-rot agaricomycete Pleurotus ostreatus.
Topics: Antifungal Agents; Flucytosine; Fungal Proteins; Gene Knockout Techniques; Genetic Markers; Genetics, Microbial; Orotic Acid; Peroxidases; Pleurotus; Selection, Genetic | 2016 |
Putative orotate transporter of Cryptococcus neoformans, Oat1, is a member of the NCS1/PRT transporter super family and its loss causes attenuation of virulence.
Topics: Biological Transport; Cryptococcus neoformans; Gene Expression Regulation, Fungal; Membrane Transport Proteins; Mutation; Nitrogen; Orotic Acid; Uracil | 2017 |
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast.
Topics: Alleles; Histones; Homologous Recombination; Mutagenesis, Site-Directed; Open Reading Frames; Orotic Acid; Plasmids; Polymerase Chain Reaction; Saccharomyces cerevisiae Proteins; Saccharomycetales | 2017 |
Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus.
Topics: Aspergillus; Cellulases; Cellulose; Dipeptidyl Peptidase 4; DNA, Bacterial; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Mutagenesis, Insertional; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Recombinant Fusion Proteins; Signal Transduction; Xylose | 2017 |
Targeted gene replacement at the URA3 locus of the basidiomycetous yeast Pseudozyma antarctica and its transformation using lithium acetate treatment.
Topics: Acetates; Amino Acid Sequence; DNA, Fungal; Drug Resistance, Fungal; Electroporation; Fungal Proteins; Hot Temperature; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Plasmids; Streptothricins; Targeted Gene Repair; Transformation, Genetic; Trees; Ustilaginales | 2017 |
A Genetic System for the Thermophilic Acetogenic Bacterium Thermoanaerobacter kivui.
Topics: Carbon Cycle; Energy Metabolism; Fructose; Gene Deletion; Genome, Bacterial; Glucose; Homologous Recombination; Mannose; Orotic Acid; Phosphofructokinases; Thermoanaerobacter | 2018 |
Establishment of a selection marker recycling system for sequential transformation of the plant-pathogenic fungus Colletotrichum orbiculare.
Topics: Colletotrichum; Cucurbitaceae; Fungal Proteins; Orotic Acid; Plant Diseases; Virulence | 2019 |
Induction of a Toxin-Antitoxin Gene Cassette under High Hydrostatic Pressure Enables Markerless Gene Disruption in the Hyperthermophilic Archaeon
Topics: Adaptation, Physiological; Antitoxins; Archaea; Archaeal Proteins; Bacterial Proteins; Base Sequence; DNA-Binding Proteins; DNA, Archaeal; Gene Expression Regulation, Archaeal; Genes, Archaeal; Hot Temperature; Hydrostatic Pressure; Hydrothermal Vents; Hydroxymethylglutaryl CoA Reductases; Membrane Glycoproteins; Orotic Acid; Promoter Regions, Genetic; Pyrococcus; Toxins, Biological; Transformation, Genetic | 2019 |
CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar.
Topics: CRISPR-Associated Protein 9; CRISPR-Cas Systems; Genes, Fungal; Genetic Vectors; Orotate Phosphoribosyltransferase; Orotic Acid; Point Mutation; Rhizopus; Uracil | 2019 |
Broad antifungal resistance mediated by RNAi-dependent epimutation in the basal human fungal pathogen Mucor circinelloides.
Topics: Antifungal Agents; Drug Resistance, Multiple, Fungal; Epigenesis, Genetic; Genes, Fungal; Humans; Mucor; Mucormycosis; Mutation; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; RNA Interference; RNA, Fungal; Sirolimus; Tacrolimus | 2019 |
Metabolic engineering generates a transgene-free safety switch for cell therapy.
Topics: Animals; Base Sequence; Cell Proliferation; Cell- and Tissue-Based Therapy; Gene Editing; Gene Targeting; Genome, Human; Humans; K562 Cells; Male; Metabolic Engineering; Mice; Multienzyme Complexes; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Pluripotent Stem Cells; Transgenes; Uridine | 2020 |
Development of a
Topics: Gene Deletion; Gene Knockout Techniques; Genetic Complementation Test; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Streptomyces rimosus | 2021 |