5-ethynyl-2--deoxyuridine has been researched along with cyanine-dye-3* in 1 studies
1 other study(ies) available for 5-ethynyl-2--deoxyuridine and cyanine-dye-3
| Article | Year |
|---|---|
A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.
The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is increasingly used for detection of various macromolecules and metabolites in biological samples. Here, we present a detailed analysis of the CuAAC reaction conditions in cells and tissue sections. Using the optimized CuAAC conditions, we have devised a highly sensitive immunostaining technique, based on the tyramide signal amplification/catalyzed reporter deposition (TSA/CARD) method with a novel alkyne tyramide substrate. The described method offers improved detection threshold compared to conventional immunofluorescent staining and produces significantly lower non-specific background than TSA/CARD with fluorescent tyramides. Topics: Animals; Azides; Boron Compounds; Brain Chemistry; Bromodeoxyuridine; Carbocyanines; Cells, Cultured; Click Chemistry; Copper; Deoxyuridine; DNA; Female; Fluorescent Antibody Technique; Fluorescent Dyes; Horseradish Peroxidase; Humans; Male; Mice; Pluripotent Stem Cells; Sensitivity and Specificity; Tyramine | 2019 |