5-7-dihydroxychromone and naringenin

To study the chemical constituents from herbs of Erigeron breviscapus.. The compounds were isolated and purified by various chromatographic techniques. Their structures were identified on the basis of physicochemical properties and spectral analysis.. Twelve compounds were isolated and structurally identified as quercetin-3-O-beta-D-glucoside (1), 5, 7-dihydroxychromone (2), 3-O-caffeoyl-gamma-quinide (3), naringenin (4), 3, 5-di-O-caffeoylquinic acid (5), 3,4-di-O-caffeoylquinic acid (6), 4, 5-di-O-caffeoylquinic acid (7), 1,3-di-O-caffeoylquinic acid (8), 1, 5-di-O-caffeoylquinic acid (9), 3-O-caffeoylquinic acid (10), 4-O-caffeoylquinic acid (11) and chlorogenic acid (12).. Compounds 1-4 were isolated from this plant for the first time.

Topics: Chlorogenic Acid; Chromatography; Chromones; Erigeron; Flavanones; Magnetic Resonance Spectroscopy; Mass Spectrometry; Plants, Medicinal; Quercetin; Quinic Acid

A new method was developed for the simultaneous determination of nine major constituents in Dracocephalum rupestre, including 5,7-dihydroxychromone (1), eriodictyol-7-O-beta-d-glucoside (2), luteolin-7-O-beta-d-glucoside (3), naringenin-7-O-beta-d-glucoside (4), apigenin-7-O-beta-d-glucoside (5), eriodictyol (6), luteolin (7), naringenin (8) and apigenin (9). The quantitative determination was conducted by reversed phase high-performance liquid chromatography with photodiode array detector (LC-PDA). Separation was performed on an Agilent Eclipse XDB-C(18) column (150 mm x 4.6 mm i.d., 5 microm) with gradient elution of acetonitrile and 0.5% aqueous acetic acid. The components were identified by retention time, ultraviolet (UV) spectra and quantified by LC-PDA at 260 nm. All calibration curves showed good linearity (r(2)>0.999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and R.S.D. values were less than 3.0%. The recoveries were between 95.15 and 104.45%. The limits of detection (LOD) ranged from 0.002 to 0.422 microg/ml and limits of quantification (LOQ) ranged from 0.005 to 1.208 microg/ml, respectively. The identity of the peaks was further confirmed by high-performance liquid chromatography with triple-quadrupole mass spectrometry system coupled with electrospray ionization (ESI) interface. The developed method was applied to the determination of nine constituents in 14 samples of D. rupestre collected at various harvesting times. Most compounds accumulated at much higher amounts in about June-July. The satisfactory results indicated that the developed method was readily utilized as a quality control method for D. rupestre.

Topics: Apigenin; Calibration; Chromatography, High Pressure Liquid; Chromones; Drug Stability; Drugs, Chinese Herbal; Flavanones; Glucosides; Luteolin; Medicine, Chinese Traditional; Molecular Structure; Plant Components, Aerial; Plant Extracts; Quality Control; Reference Standards; Reproducibility of Results; Seasons; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Temperature; Time Factors

To study the chemical constituents of the fruits of Polygonum orientale L., silica gel and ODS column chromatography methods were used to separate and purify the constituents. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Three compounds were identified as 28-O-beta-D-glucopyranosyl-3beta, 7beta-dihydroxy-lup-20 (29)-en-28-oate (1), 5,7-dihydroxychromone (2) and naringenin (3). Compound 1 is a new triterpenoid saponin and others were isolated from the fruits of this plant for the first time.

Topics: Chromones; Flavanones; Fruit; Molecular Structure; Plants, Medicinal; Polygonum; Saponins; Triterpenes