5-7-dihydroxychromone and eriodictyol

A new method was developed for the simultaneous determination of nine major constituents in Dracocephalum rupestre, including 5,7-dihydroxychromone (1), eriodictyol-7-O-beta-d-glucoside (2), luteolin-7-O-beta-d-glucoside (3), naringenin-7-O-beta-d-glucoside (4), apigenin-7-O-beta-d-glucoside (5), eriodictyol (6), luteolin (7), naringenin (8) and apigenin (9). The quantitative determination was conducted by reversed phase high-performance liquid chromatography with photodiode array detector (LC-PDA). Separation was performed on an Agilent Eclipse XDB-C(18) column (150 mm x 4.6 mm i.d., 5 microm) with gradient elution of acetonitrile and 0.5% aqueous acetic acid. The components were identified by retention time, ultraviolet (UV) spectra and quantified by LC-PDA at 260 nm. All calibration curves showed good linearity (r(2)>0.999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and R.S.D. values were less than 3.0%. The recoveries were between 95.15 and 104.45%. The limits of detection (LOD) ranged from 0.002 to 0.422 microg/ml and limits of quantification (LOQ) ranged from 0.005 to 1.208 microg/ml, respectively. The identity of the peaks was further confirmed by high-performance liquid chromatography with triple-quadrupole mass spectrometry system coupled with electrospray ionization (ESI) interface. The developed method was applied to the determination of nine constituents in 14 samples of D. rupestre collected at various harvesting times. Most compounds accumulated at much higher amounts in about June-July. The satisfactory results indicated that the developed method was readily utilized as a quality control method for D. rupestre.

Topics: Apigenin; Calibration; Chromatography, High Pressure Liquid; Chromones; Drug Stability; Drugs, Chinese Herbal; Flavanones; Glucosides; Luteolin; Medicine, Chinese Traditional; Molecular Structure; Plant Components, Aerial; Plant Extracts; Quality Control; Reference Standards; Reproducibility of Results; Seasons; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Temperature; Time Factors

To investigate chemical constituents of the stem of Viscum nudum and their bioacyivity.. The major chemical constituents were isolated from the AcOEt-solved part of ethanol-extract of the plant by column chromatography and the active screening test in vitro were taken out for looking for compounds to acccelerate PC12 cell differentiation.. 5 compounds were identified as eriodictyol (1), 5, 7-dihydroxy-3', 4'-dimethoxy flavanone (2), oleanolic (3), 5, 7-dihydroxychromone (4) and homeriodictyol (5) by spectral evidences, in which homeriodictyol (5) had acceleration differentiation to PC12 cell.. All compounds were obtained from this plant for the first time, and bioactive constituent was observed in the AcOEt-solved part.

Topics: Animals; Cell Differentiation; Chromones; Flavanones; Flavones; Oleanolic Acid; PC12 Cells; Plant Stems; Plants, Medicinal; Rats; Viscum