5,6-dihydroorotate has been researched along with orotic acid in 59 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 15 (25.42) | 18.7374 |
| 1990's | 10 (16.95) | 18.2507 |
| 2000's | 20 (33.90) | 29.6817 |
| 2010's | 13 (22.03) | 24.3611 |
| 2020's | 1 (1.69) | 2.80 |
| Authors | Studies |
|---|---|
| Christopherson, RI; Jones, ME | 2 |
| Dave, D; Gutteridge, WE; Richards, WH | 1 |
| Krungkrai, J | 1 |
| Cerami, A; Henderson, GB; Krungkrai, J | 1 |
| Ishida, R; Matsumoto, K; Sugano, H; Suzuki, M; Yamamura, M | 1 |
| Bidigare, RR; Choudhury, RR; Mehta, BJ; Pettigrew, DW; Sander, EG; Scheffler, JE | 1 |
| Cowling, RA; Lee, MH; Pettigrew, DW; Sander, EG | 1 |
| Eichler, W; Schertel, B | 1 |
| Gravel, D; Grégoire, M; Morais, R; Quilliam, MA | 1 |
| Pascal, RA; Walsh, CT | 1 |
| Levenson, CH; Meyer, RB | 1 |
| Choie, DD; Cooney, DA; Jayaram, HN; Kensler, TW; Schaeffer, C | 1 |
| Coombs, GH; Gero, AM | 1 |
| Cooney, DA; Han, N; Kensler, TW | 1 |
| Durschlag, RP; Robinson, JL | 1 |
| Cleaveland, ES; Cooney, DA; Ford, H; Kelley, JA; Paull, K; Zaharevitz, DW | 1 |
| Altekruse, D; Gonski, S; Knecht, W; Löffler, M; Rotgeri, A | 1 |
| Björnberg, O; Jensen, KF; Larsen, S; Rowland, P | 1 |
| Bloch, JC; Exinger, F; Pfeiffer, P | 1 |
| Flynn, PJ; Reece, RJ | 1 |
| Carrey, EA; Fairbanks, LD; Kirschbaum, B; Rückemann, K; Simmonds, HA; Swaminathan, R | 1 |
| Copeland, RA; Jiang, W; Kopcho, LM; Locke, G; Marcinkeviciene, J; Rogers, MJ | 1 |
| Bisaha, JJ; Jordan, DB; Picollelli, MA | 1 |
| Grossman, TH; Hedstrom, L; McLean, JE; Neidhardt, EA; Punreddy, SR | 1 |
| Gaill, F; Hervé, G; Minic, Z; Penverne, B; Simon, V | 1 |
| Björnberg, O; Jensen, KF; Jordan, DB; Palfey, BA | 1 |
| Amino, H; Kita, K; Mi-ichi, F; Takamiya, S; Takashima, E; Takeo, S | 1 |
| TAYLOR, ML; TAYLOR, WH | 1 |
| NOVELLI, GD; TAYLOR, WH | 1 |
| Antal, T; Hansen, M; Johansson, E; Larsen, S; Le Nours, J; Löffler, M; Ullrich, A | 1 |
| Jensen, KF; Mohsen, AW; Munro, AW; Rigby, SE; Scrutton, NS | 1 |
| Li, Y; Porter, TN; Raushel, FM | 1 |
| Chan, CW; Christopherson, RI; Lee, M; Maher, MJ; Mitchell Guss, J | 1 |
| Annoura, T; Aoki, T; Hashimoto, M; Iizumi, K; Kita, K; Makiuchi, T; Murata, E; Nara, T; Sariego, I; Tsubouchi, A | 1 |
| Basran, J; Combe, JP; Hothi, P; Leys, D; Munro, AW; Rigby, SE; Scrutton, NS | 1 |
| Anderson, MA; Chan, C; Christopherson, RI; Cleland, WW; Huang, DT; Shojaei, M | 1 |
| Christopherson, RI; Huang, DT; Kaplan, J; Katis, VL; Menz, RI; Wake, RG; Wolfenden, R; Zhao, F | 1 |
| Fagan, RL; Nelson, MN; Pagano, PM; Palfey, BA | 1 |
| Baker, KE; Kelln, RA; Krogan, NJ; Lamb, SS; Neuhard, J; Zaharik, ML | 1 |
| Al-Karadaghi, S; Dahlberg, L; Dufe, VT; Fritzson, I; Khairoullina, A; Svensson, B; Walse, B; Wellmar, U | 1 |
| Aoki, T; Harada, S; Inaoka, DK; Kita, K; Kurisu, G; Nara, T; Sakamoto, K; Shiba, T; Shimizu, H | 1 |
| Brunzelle, JS; Edwards, BF; Evans, DR; Fernando, R; Guy-Evans, HI; Martin, PD; Purcarea, C; Vaishnav, A; Zhang, P | 1 |
| Fagan, RL; Palfey, BA | 1 |
| Cheleski, J; Citadini, AP; da Costa Filho, AJ; Montanari, CA; Nonato, MC; Wiggers, HJ | 1 |
| Harada, S; Inaoka, DK; Kita, K | 1 |
| McDonald, CA; Palfey, BA | 1 |
| Costa-Filho, AJ; Couto, SG; Cristina Nonato, M | 1 |
| Bzik, DJ; Carruthers, VB; Fox, BA; Garavito, MF; Hortua Triana, MA; Huynh, MH; Löffler, M; Zimmermann, BH | 1 |
| Anderson, E; Ansari, M; Bengani, H; Branney, P; Campbell, L; Fitzpatrick, DR; Gener Querol, B; Gordon, C; Kirk, J; Kohlhase, J; Lam, W; Lees, M; Lengfeld, T; McKay, S; McKenzie, K; Medina, B; Mercer, C; Morrison, H; Rainger, J; Riess, A; Robertson, M; Smithson, S; Sokhi, K; Wieczorek, D | 1 |
| Bohne, W; Gross, U; Hegewald, J | 1 |
| Alves, CN; Lameira, J; Martí, S; Silva, Nde F | 1 |
| Huang, CY; Peng, WF | 1 |
| Alves, CN; Roitberg, AE; Silva, JR | 1 |
| Bamshad, MJ; Carpenter, KH; Duley, JA; Henman, MG; Marshall, GA; Ooi, CY; Pinner, JR; Wilcken, B | 1 |
| Dubey, VK; Kumar, R; Tiwari, K | 1 |
| Bai, C; Chu, Q; Li, B; Liu, M; Qiu, L; Ruan, Y; Wang, Y; Yang, C | 1 |
| Bellucci, MC; Sacchetti, A; Volonterio, A | 1 |
| Gornbein, JA; Koos, BJ | 1 |
1 review(s) available for 5,6-dihydroorotate and orotic acid
| Article | Year |
|---|---|
[Development of anti-trypanosome drugs targeting nucleotides biosynthesis and red-ox regulatory pathway].
Topics: Animals; Dihydroorotate Dehydrogenase; Drug Design; Energy Metabolism; Fumarates; Nucleotides; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Oxonic Acid; Protein Conformation; Succinic Acid; Trypanocidal Agents; Trypanosoma cruzi | 2009 |
58 other study(ies) available for 5,6-dihydroorotate and orotic acid
| Article | Year |
|---|---|
Interconversion of carbamayl-L-aspartate and L-dihydroorotate by dihydroorotase from mouse Ehrlich ascites carcinoma.
Topics: Amidohydrolases; Animals; Aspartate Carbamoyltransferase; Aspartic Acid; Carbamates; Carcinoma, Ehrlich Tumor; Diethyl Pyrocarbonate; Dihydroorotase; Hydrogen-Ion Concentration; Kinetics; Mathematics; Mice; Orotic Acid | 1979 |
Conversion of dihydroorotate to orotate in parasitic protozoa.
Topics: Animals; Antimalarials; Crithidia; Dihydroorotate Oxidase; Enzyme Inhibitors; Kinetics; Leishmania; Liver; Orotic Acid; Oxygen Consumption; Plasmodium; Rats; Trypanosoma | 1979 |
Malarial dihydroorotate dehydrogenase mediates superoxide radical production.
Topics: 2,6-Dichloroindophenol; Animals; Cytochrome c Group; Dihydroorotate Oxidase; Electrophoresis, Polyacrylamide Gel; Mitochondria; Nitroblue Tetrazolium; Orotic Acid; Oxidation-Reduction; Plasmodium berghei; Plasmodium falciparum; Superoxide Dismutase; Superoxides | 1991 |
Pyrimidine biosynthesis in parasitic protozoa: purification of a monofunctional dihydroorotase from Plasmodium berghei and Crithidia fasciculata.
Topics: Animals; Aspartic Acid; Chromatography, Gel; Crithidia; Dihydroorotase; Dimethyl Sulfoxide; Kinetics; Molecular Weight; Orotic Acid; Plasmodium berghei; Pyrimidines | 1990 |
Synthesis and central nervous system actions of thyrotropin-releasing hormone analogues containing a dihydroorotic acid moiety.
Topics: Anesthesia; Central Nervous System; Chemical Phenomena; Chemistry; Hypothermia; Molecular Structure; Motor Activity; Orotic Acid; Pentobarbital; Reserpine; Stereoisomerism; Structure-Activity Relationship; Thyrotropin; Thyrotropin-Releasing Hormone | 1990 |
Enzyme elements involved in the interconversion of L-carbamylaspartate and L-dihydroorotate by dihydroorotase from Clostridium oroticum.
Topics: Amidohydrolases; Aspartic Acid; Catalysis; Clostridium; Cobalt; Cyclization; Diethyl Pyrocarbonate; Dihydroorotase; Histidine; Hydrogen-Ion Concentration; Kinetics; Orotic Acid; Zinc | 1985 |
Bovine liver dihydropyrimidine amidohydrolase: pH dependencies of inactivation by chelators and steady-state kinetic properties.
Topics: Amidohydrolases; Animals; Binding, Competitive; Cattle; Chelating Agents; Hydrogen-Ion Concentration; Kinetics; Liver; Orotic Acid; Oxyquinoline; Phenanthrolines; Picolinic Acids; Uracil | 1986 |
Dihydroorotate induces Ca2+ release from rat liver mitochondria: a contribution to the mechanism of alloxan-induced Ca2+ release.
Topics: Alloxan; Animals; Calcium; Kinetics; Mitochondria, Liver; Orotic Acid; Oxidation-Reduction; Potassium Cyanide; Rats | 1988 |
On auxotrophy for pyrimidines of respiration-deficient chick embryo cells.
Topics: Animals; Aspartate Carbamoyltransferase; Cell Division; Chick Embryo; Chloramphenicol; Culture Media; Dihydroorotate Oxidase; Electron Transport; Multienzyme Complexes; Orotic Acid; Oxygen; Pyrimidines | 1984 |
Mechanistic studies with deuterated dihydroorotates on the dihydroorotate oxidase from Crithidia fasciculata.
Topics: Animals; Crithidia; Deuterium; Dihydroorotate Oxidase; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Magnetic Resonance Spectroscopy; Orotic Acid; Oxidoreductases | 1984 |
The overall synthesis of L-5,6-dihydroorotate by multienzymatic protein pyr1-3 from hamster cells. Kinetic studies, substrate channeling, and the effects of inhibitors.
Topics: Amidohydrolases; Animals; Aspartate Carbamoyltransferase; Carbamoyl-Phosphate Synthase (Ammonia); Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); Cell Line; Cricetinae; Dihydroorotase; Hydrogen-Ion Concentration; Kinetics; Ligases; Mesocricetus; Mice; Multienzyme Complexes; Neoplasm Proteins; Orotic Acid; Substrate Specificity | 1980 |
Design and synthesis of tetrahedral intermediate analogues as potential dihydroorotase inhibitors.
Topics: Amidohydrolases; Animals; Chemical Phenomena; Chemistry; Cricetinae; Dihydroorotase; Heterocyclic Compounds; Orotic Acid; Structure-Activity Relationship; Sulfonamides; Sulfoxides; Sulfur | 1984 |
A facile tritium release assay for mammalian L-dihydroorotate dehydrogenase.
Topics: Animals; Cricetinae; Dihydroorotate Oxidase; Kinetics; Methods; Mice; Mitochondria, Liver; Orotic Acid; Oxidoreductases; Tritium; Water | 1981 |
Leishmania mexicana: conversion of dihydroorotate to orotate in amastigotes and promastigotes.
Topics: Coenzymes; Dihydroorotate Oxidase; Enzyme Activation; Hydrogen-Ion Concentration; Kinetics; Leishmania; Orotic Acid; Oxidoreductases; Potassium Chloride; Pyrimidines; Subcellular Fractions | 1982 |
A straightforward method for the simultaneous preparation of radiolabeled L-dihydroörotic and N-carbamyl-L-aspartic acids.
Topics: Aspartic Acid; Carbon Radioisotopes; Chromatography, Ion Exchange; Electrophoresis; Isotope Labeling; NAD; Orotic Acid | 1981 |
Orotic acid-induced metabolic changes in the rat.
Topics: Animals; Dose-Response Relationship, Drug; Female; Lipid Metabolism; Liver; Nucleotides; Organ Size; Orotic Acid; Purines; Pyrimidines; Rats; Uridine | 1980 |
Identification of a novel inhibitor (NSC 665564) of dihydroorotate dehydrogenase with a potency equivalent to brequinar.
Topics: Antineoplastic Agents; Aspartic Acid; Biphenyl Compounds; Breast Neoplasms; Carbolines; Carcinoma, Non-Small-Cell Lung; Central Nervous System Neoplasms; Colonic Neoplasms; Dihydroorotate Dehydrogenase; Enzyme Inhibitors; Female; Humans; Kidney Neoplasms; Kinetics; Leukemia; Lung Neoplasms; Male; Melanoma; Mitochondria; Orotic Acid; Ovarian Neoplasms; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Prostatic Neoplasms; Ribonucleotides; Sodium Bicarbonate; Software; Tumor Cells, Cultured | 1996 |
Rat dihydroorotate dehydrogenase: isolation of the recombinant enzyme from mitochondria of insect cells.
Topics: Amino Acid Sequence; Animals; Blotting, Western; Cell Line; Chromatography, Ion Exchange; Dihydroorotate Dehydrogenase; DNA, Complementary; Genetic Vectors; Humans; Kinetics; Liver; Mitochondria; Molecular Sequence Data; Moths; Nucleopolyhedroviruses; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Rats; Recombinant Fusion Proteins; Spodoptera; Substrate Specificity; Ubiquinone | 1997 |
Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis.
Topics: Alkylating Agents; Binding Sites; Cysteine; Dihydroorotate Dehydrogenase; Dithionitrobenzoic Acid; Enzyme Inhibitors; Evolution, Molecular; Flavoproteins; Hydrogen-Ion Concentration; Kinetics; Lactococcus lactis; Mutagenesis, Site-Directed; Orotic Acid; Oxidation-Reduction; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Spectrophotometry | 1997 |
Dihydroorotate (dhout) and orotate (orout) utilizer mutants in yeast: identification of the dhout mutation and allelism of the DHO and URE2 genes.
Topics: Alleles; Chromosome Mapping; Cloning, Molecular; Fungal Proteins; Glutathione Peroxidase; Mutagenesis; Orotic Acid; Prions; Pyrimidines; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Ultraviolet Rays | 1998 |
Activation of transcription by metabolic intermediates of the pyrimidine biosynthetic pathway.
Topics: Animals; Binding Sites; Cell Line; DNA; DNA-Binding Proteins; Fungal Proteins; Orotic Acid; Pyrimidines; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcriptional Activation | 1999 |
Simultaneous separation by high-performance liquid chromatography of carbamoyl aspartate, carbamoyl phosphate and dihydroorotic acid.
Topics: Anti-Inflammatory Agents, Non-Steroidal; Aspartic Acid; Biphenyl Compounds; Carbamyl Phosphate; Chromatography, High Pressure Liquid; Humans; Immunosuppressive Agents; Isoxazoles; Leflunomide; Orotic Acid; T-Lymphocytes | 1999 |
A second dihydroorotate dehydrogenase (Type A) of the human pathogen Enterococcus faecalis: expression, purification, and steady-state kinetic mechanism.
Topics: Amino Acid Sequence; Barbiturates; Binding Sites; Catalysis; Cloning, Molecular; Dihydroorotate Dehydrogenase; Enterococcus faecalis; Enzyme Stability; Escherichia coli; Fumarates; Humans; Kinetics; Models, Chemical; Molecular Sequence Data; Molecular Weight; Orotic Acid; Oxidation-Reduction; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Oxygen; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Thermodynamics; Titrimetry; Vitamin K | 2000 |
Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: studies on pH, alternate substrates, and inhibitors.
Topics: Binding Sites; Carbon Radioisotopes; Catalysis; Deuterium; Dihydroorotate Dehydrogenase; Electron Transport; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Saccharomyces cerevisiae; Substrate Specificity | 2000 |
Expression and characterization of E. coli-produced soluble, functional human dihydroorotate dehydrogenase: a potential target for immunosuppression.
Topics: Animals; Cattle; Cloning, Molecular; Dihydroorotate Dehydrogenase; Escherichia coli; Histidine; Humans; Immunosuppression Therapy; Kinetics; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Recombinant Fusion Proteins; Solubility; Subcellular Fractions; Ubiquinone | 1999 |
Contribution of the bacterial endosymbiont to the biosynthesis of pyrimidine nucleotides in the deep-sea tube worm Riftia pachyptila.
Topics: Animals; Aspartate Carbamoyltransferase; Bacteria; Bacterial Proteins; Carbamoyl-Phosphate Synthase (Ammonia); Chromatography, Ion Exchange; Dihydroorotase; Glutamate-Ammonia Ligase; Invertebrates; Models, Biological; Nitrate Reductase; Nitrate Reductases; Orotic Acid; Pyrimidine Nucleotides; Seawater; Symbiosis; Tissue Extracts | 2001 |
Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis.
Topics: Binding Sites; Catalysis; Cysteine; Dihydroorotate Dehydrogenase; Escherichia coli; Fumarates; Lactobacillus; Mutation; Orotic Acid; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Recombinant Proteins; Serine; Substrate Specificity | 2001 |
Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration.
Topics: Animals; Blotting, Western; Fumarates; Mitochondria; Orotic Acid; Oxygen; Oxygen Consumption; Plasmodium falciparum; Succinate Dehydrogenase | 2001 |
ENZYMES OF THE PYRIMIDINE PATHWAY IN ESCHERICHIA COLI. II. INTRACELLULAR LOCALIZATION AND PROPERTIES OF DIHYDROOROTIC DEHYDROGENASE.
Topics: Carboxy-Lyases; Cholagogues and Choleretics; Cyanides; Cytochromes; Escherichia coli; Ferricyanides; Metabolism; Nucleotides; Orotic Acid; Oxidoreductases; Pharmacology; Pyrimidines; Research; Transferases | 1964 |
ENZYMES OF THE PYRIMIDINE PATHWAY IN ESCHERICHIA COLI. I. SYNTHESIS BY CELLS AND SPHEROPLASTS.
Topics: Amino Acids; Chloramphenicol; Edetic Acid; Escherichia coli; Nucleotides; Ornithine; Ornithine Carbamoyltransferase; Orotic Acid; Oxidoreductases; Pharmacology; Pyrimidines; Research; Spheroplasts; Sucrose; Transferases; Uracil | 1964 |
Inhibitor binding in a class 2 dihydroorotate dehydrogenase causes variations in the membrane-associated N-terminal domain.
Topics: Amino Acid Sequence; Aniline Compounds; Animals; Atovaquone; Biphenyl Compounds; Catalysis; Crotonates; Crystallography, X-Ray; Dihydroorotate Dehydrogenase; Drug Design; Enzyme Inhibitors; Hydrogen Bonding; Hydroxybutyrates; Immunosuppressive Agents; Models, Molecular; Molecular Sequence Data; Molecular Structure; Naphthoquinones; Nitriles; Orotic Acid; Oxidoreductases Acting on CH-CH Group Donors; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Rats; Sequence Alignment; Substrate Specificity; Toluidines | 2004 |
Thermodynamic basis of electron transfer in dihydroorotate dehydrogenase B from Lactococcus lactis: analysis by potentiometry, EPR spectroscopy, and ENDOR spectroscopy.
Topics: Dihydroorotate Dehydrogenase; Dimerization; Electron Spin Resonance Spectroscopy; Electron Transport; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Flavins; Lactococcus lactis; NAD; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Potentiometry; Protein Subunits; Thermodynamics; Titrimetry | 2004 |
Mechanism of the dihydroorotase reaction.
Topics: Deuterium; Dihydroorotase; Escherichia coli; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Orotic Acid; Spectrophotometry; Sulfur; Zinc | 2004 |
Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits.
Topics: Amino Acid Sequence; Animals; Binding Sites; Crystallography, X-Ray; Dihydroorotase; Escherichia coli Proteins; Humans; Models, Molecular; Molecular Sequence Data; Molecular Structure; Orotic Acid; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; Protein Subunits; Sequence Alignment | 2005 |
Genetic diversity and kinetic properties of Trypanosoma cruzi dihydroorotate dehydrogenase isoforms.
Topics: Animals; Base Sequence; Dihydroorotate Dehydrogenase; Fumarates; Genetic Variation; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Molecular Sequence Data; Orotic Acid; Oxidoreductases Acting on CH-CH Group Donors; Point Mutation; Polymorphism, Genetic; Recombinant Proteins; Sequence Alignment; Temperature; Trypanosoma cruzi | 2006 |
Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis.
Topics: Catalysis; Dihydroorotate Dehydrogenase; Dimerization; Electrochemistry; Electron Transport; Flavin Mononucleotide; Flavins; Lactococcus lactis; Lysine; Mutagenesis, Site-Directed; NAD; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Structure, Tertiary; Tyrosine | 2006 |
13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus.
Topics: Animals; Aspartic Acid; Base Sequence; Carbon Isotopes; Cricetinae; DNA Primers; Kinetics; Mass Spectrometry; Nitrogen Isotopes; Orotic Acid | 2006 |
Thermodynamic analysis of catalysis by the dihydroorotases from hamster and Bacillus caldolyticus, as compared with the uncatalyzed reaction.
Topics: Amino Acid Sequence; Animals; Bacillus; Catalysis; Cricetinae; Dihydroorotase; Enzyme Activation; Hydrolysis; Molecular Sequence Data; Orotic Acid; Protein Denaturation; Sequence Alignment; Temperature; Thermodynamics | 2006 |
Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases.
Topics: Binding Sites; Catalysis; Dihydroorotate Dehydrogenase; Escherichia coli; Escherichia coli Proteins; Flavin Mononucleotide; Humans; Hydrogen-Ion Concentration; Models, Chemical; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Serine; Substrate Specificity | 2006 |
Mutations in yhiT enable utilization of exogenous pyrimidine intermediates in Salmonella enterica serovar Typhimurium.
Topics: Amino Acid Sequence; Asparagine; Aspartic Acid; Bacterial Proteins; Base Sequence; Carrier Proteins; Cloning, Molecular; Escherichia coli; Genes, Bacterial; Malates; Membrane Proteins; Molecular Sequence Data; Mutation; Operon; Orotic Acid; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Salmonella typhimurium; Succinic Acid; Transcription Initiation Site | 2007 |
The structures of human dihydroorotate dehydrogenase with and without inhibitor reveal conformational flexibility in the inhibitor and substrate binding sites.
Topics: Binding Sites; Biphenyl Compounds; Carboxylic Acids; Crystallography, X-Ray; Dihydroorotate Dehydrogenase; Enzyme Inhibitors; Flavin Mononucleotide; Humans; Naphthalenes; Orotic Acid; ortho-Aminobenzoates; Oxidoreductases Acting on CH-CH Group Donors; Protein Binding; Protein Structure, Secondary; Recombinant Proteins; Ubiquinone | 2008 |
Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction.
Topics: Amino Acid Sequence; Animals; Cloning, Molecular; Crystallography, X-Ray; Dihydroorotate Dehydrogenase; Fumarates; Models, Molecular; Molecular Sequence Data; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Conformation; Sequence Homology, Amino Acid; Substrate Specificity; Trypanosoma cruzi | 2008 |
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.
Topics: Allosteric Regulation; Aspartate Carbamoyltransferase; Bacteria; Bacterial Proteins; Binding Sites; Crystallography, X-Ray; Dihydroorotase; Multienzyme Complexes; Orotic Acid; Protein Structure, Tertiary; Pyrimidines; Static Electricity; Thermodynamics | 2009 |
Roles in binding and chemistry for conserved active site residues in the class 2 dihydroorotate dehydrogenase from Escherichia coli.
Topics: Catalytic Domain; Dihydroorotate Dehydrogenase; Escherichia coli; Escherichia coli Proteins; Hydrogen Bonding; Molecular Structure; Mutagenesis, Site-Directed; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Binding | 2009 |
Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry.
Topics: Biocatalysis; Calorimetry; Dihydroorotate Dehydrogenase; Dimethyl Sulfoxide; Enzyme Assays; Fumarates; Kinetics; Octoxynol; Orotic Acid; Oxidoreductases Acting on CH-CH Group Donors; Recombinant Proteins; Substrate Specificity; Thermodynamics; Trypanosoma cruzi | 2010 |
Substrate binding and reactivity are not linked: grafting a proton-transfer network into a Class 1A dihydroorotate dehydrogenase.
Topics: Bacterial Proteins; Binding Sites; Biocatalysis; Dihydroorotate Dehydrogenase; Kinetics; Lactococcus lactis; Models, Chemical; Models, Molecular; Molecular Structure; Mutation; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Binding; Protein Structure, Tertiary; Protons; Substrate Specificity | 2011 |
Site directed spin labeling studies of Escherichia coli dihydroorotate dehydrogenase N-terminal extension.
Topics: Catalysis; Dihydroorotate Dehydrogenase; Electron Spin Resonance Spectroscopy; Escherichia coli; Magnetic Fields; Orotic Acid; Oxidoreductases Acting on CH-CH Group Donors; Protein Structure, Tertiary; Spin Labels | 2011 |
Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii.
Topics: Amino Acid Sequence; Biosynthetic Pathways; Cloning, Molecular; Conserved Sequence; Dihydroorotate Dehydrogenase; Enzyme Inhibitors; Gene Knockout Techniques; Kinetics; Mitochondria; Molecular Sequence Data; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Sorting Signals; Protein Transport; Proteolysis; Protozoan Proteins; Pyrimidines; Toxoplasma | 2012 |
Miller (Genee-Wiedemann) syndrome represents a clinically and biochemically distinct subgroup of postaxial acrofacial dysostosis associated with partial deficiency of DHODH.
Topics: Abnormalities, Multiple; Animals; Base Sequence; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); Child, Preschool; Dihydroorotate Dehydrogenase; DNA Mutational Analysis; Embryo, Mammalian; Female; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation, Developmental; Genetic Association Studies; Genetic Complementation Test; Humans; Infant; Limb Buds; Limb Deformities, Congenital; Male; Mandibulofacial Dysostosis; Mice; Micrognathism; Multienzyme Complexes; Mutation, Missense; Orotate Phosphoribosyltransferase; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Oxidoreductases Acting on CH-CH Group Donors; Pedigree; Reference Standards; Schizosaccharomyces; Schizosaccharomyces pombe Proteins | 2012 |
Identification of dihydroorotate dehydrogenase as a relevant drug target for 1-hydroxyquinolones in Toxoplasma gondii.
Topics: Binding Sites; Dihydroorotate Dehydrogenase; Enzyme Inhibitors; Humans; Inhibitory Concentration 50; Kinetics; Orotic Acid; Oxidoreductases Acting on CH-CH Group Donors; Point Mutation; Protozoan Proteins; Quinolones; Toxoplasma; Toxoplasmosis | 2013 |
Computational study of the mechanism of half-reactions in class 1A dihydroorotate dehydrogenase from Trypanosoma cruzi.
Topics: Biocatalysis; Dihydroorotate Dehydrogenase; Hydrogen Bonding; Molecular Dynamics Simulation; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Quantum Theory; Static Electricity; Trypanosoma cruzi | 2013 |
Allantoinase and dihydroorotase binding and inhibition by flavonols and the substrates of cyclic amidohydrolases.
Topics: Amidohydrolases; Bacterial Proteins; Binding, Competitive; Catalytic Domain; Dihydroorotase; Enzyme Inhibitors; Hydantoins; Kaempferols; Klebsiella pneumoniae; Models, Molecular; Orotic Acid; Phthalimides; Protein Binding; Salmonella typhimurium; Substrate Specificity | 2014 |
Insights into the mechanism of oxidation of dihydroorotate to orotate catalysed by human class 2 dihydroorotate dehydrogenase: a QM/MM free energy study.
Topics: Binding Sites; Biocatalysis; Dihydroorotate Dehydrogenase; Flavin Mononucleotide; Humans; Molecular Dynamics Simulation; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Protein Structure, Tertiary; Quantum Theory; Thermodynamics | 2015 |
Elevated plasma dihydroorotate in Miller syndrome: Biochemical, diagnostic and clinical implications, and treatment with uridine.
Topics: Abnormalities, Multiple; Child, Preschool; Dihydroorotate Dehydrogenase; Genotype; Humans; Limb Deformities, Congenital; Male; Mandibulofacial Dysostosis; Micrognathism; Mutation; Orotic Acid; Oxidation-Reduction; Oxidoreductases Acting on CH-CH Group Donors; Uridine | 2016 |
Biochemical characterization of dihydroorotase of Leishmania donovani: Understanding pyrimidine metabolism through its inhibition.
Topics: Biotin; Cytidine Deaminase; Dihydroorotase; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression Regulation, Enzymologic; Kaempferols; Kinetics; Leishmania donovani; Molecular Docking Simulation; Molecular Structure; Orotic Acid; Protein Domains; Protozoan Proteins; Pyrimidines; Recombinant Proteins; Reverse Transcriptase Polymerase Chain Reaction; Substrate Specificity; Sulfones | 2016 |
Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.
Topics: Acetyl Coenzyme A; Ammonia; Animals; Carbon; Cell Hypoxia; Cell Line, Tumor; Cell Survival; Female; Glucose; Glutamine; HEK293 Cells; Humans; Lactic Acid; Lipogenesis; Metabolic Networks and Pathways; Metabolome; Metabolomics; Mice; Mice, Nude; Neoplasms; Nitrogen; Nucleotides; Orotic Acid; Tumor Microenvironment; Xenograft Model Antitumor Assays | 2019 |
Multicomponent Approach to Libraries of Substituted Dihydroorotic Acid Amides.
Topics: Amides; Combinatorial Chemistry Techniques; Molecular Structure; Orotic Acid; Small Molecule Libraries | 2019 |
Early pregnancy metabolites predict gestational diabetes mellitus: implications for fetal programming.
Topics: Adult; Alanine; Arginine; Carnitine; Case-Control Studies; Diabetes, Gestational; Diet Therapy; Dopamine; Early Diagnosis; Epigenesis, Genetic; Female; Fetal Development; Gestational Age; Glucose Tolerance Test; Glucuronides; Humans; Hypoglycemic Agents; Lactones; Lysine; Meglutol; Metabolomics; Neopterin; Orotic Acid; Phenols; Pregnancy; Ribonucleosides; Sulfides | 2021 |