5-10-methylenetetrahydrofolic-acid and ribothymidine

5-10-methylenetetrahydrofolic-acid has been researched along with ribothymidine* in 1 studies

Other Studies

1 other study(ies) available for 5-10-methylenetetrahydrofolic-acid and ribothymidine

ArticleYear
Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2.
    The Journal of biological chemistry, 1980, May-25, Volume: 255, Issue:10

    The methyl carbon of ribothymidine in Loop IV of the tRNA of Streptococcus faecalis, Bacillus subtilis, and some other microorganisms is derived directly from 5,10-methylenetetrahydrofolate, not S-adenosylmethionine. The pure enzyme from S. faecalis also requires FADH2. We have obtained evidence that tetrahydrofolate is a product of the reaction and demonstrated that label from [5-3H]5-deazaFMNH2 is incorporated into the methyl moiety of ribothymidine. These data indicate that the enzyme uses methylenetetrahydrofolate solely as a 1-carbon donor and employs FADH2 as a reducing agent in vitro according to the following reaction: tRNA(U psi C) + CH2 = THF + FADH2 leads to tRNA(T psi C) + THF + FAD.

    Topics: Enterococcus faecalis; Flavin-Adenine Dinucleotide; Methylation; Methyltransferases; Oxidation-Reduction; Ribonucleosides; RNA, Transfer; Species Specificity; Tetrahydrofolates; Uridine

1980