5--methoxyhydnocarpin and pheophorbide-a

5--methoxyhydnocarpin has been researched along with pheophorbide-a* in 2 studies

Other Studies

2 other study(ies) available for 5--methoxyhydnocarpin and pheophorbide-a

Article	Year
Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin. International journal of antimicrobial agents, 2003, Volume: 22, Issue:1 Previous research showed that berberine-containing Berberis species synthesise the substances 5'-methoxyhydnocarpin-D (5'-MHC-D) and pheophorbide a, which have no antimicrobial activity but inhibit the expression of multidrug resistant efflux pumps (MDRs) in Staphylococcus aureus and potentiate the action of berberine. The MDR pumps extrude synthetic and natural antimicrobials from bacterial cells. We searched for these compounds in Berberis aetnensis C. Presl. (Berberidaceae), an endemic plant of the volcano Mount Etna. This work confirms the presence of pheophorbide a and permits us to hypothesise the presence of 5'-MHC-D in leaf extracts. In fact, the activity of ciprofloxacin was improved when two chromatographic fractions isolated from leaf extracts were added. These results are indicative of the presence of MDR pump inhibitors. Moreover, crude extracts were tested on several micro-organisms and showed antimicrobial activity mainly against Gram-positive bacteria and yeasts. Topics: Anti-Bacterial Agents; Bacterial Proteins; Berberine; Berberis; Candida; Chlorophyll; Ciprofloxacin; Drug Interactions; Flavonoids; Gram-Negative Bacteria; Gram-Positive Bacteria; Molecular Structure; Multidrug Resistance-Associated Proteins; Plant Extracts; Staphylococcus aureus	2003
5'-Methoxyhydnocarpin-D and pheophorbide A: Berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus. Journal of natural products, 2000, Volume: 63, Issue:8 A new method of bioactivity-directed fractionation, based on multidrug resistant pump (MDR) inhibition in Staphylococcus aureus, was demonstrated. This resulted in the isolation, from berberine-containing Berberis species, of two compounds that are themselves devoid of S. aureus antibacterial activity, but that form potent synergistic couples with a subinhibitory concentration of berberine. The bacterial MDR pump inhibitors were identified as the flavonolignan 2 and the porphyrin 3. Another natural flavonolignan, silybin (8) from Silybum marianum, was also shown to be a bacterial MDR pump inhibitor. Topics: Berberine; Biological Assay; Chlorophyll; Chromatography, Gel; Chromatography, Thin Layer; Colorado; Drug Resistance, Microbial; Drug Resistance, Multiple; Flavonoids; Gas Chromatography-Mass Spectrometry; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Plant Leaves; Plants, Medicinal; Spectrometry, Mass, Fast Atom Bombardment; Spectrophotometry, Ultraviolet; Staphylococcus aureus	2000

Article

Year

Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin.

International journal of antimicrobial agents, 2003, Volume: 22, Issue:1

Previous research showed that berberine-containing Berberis species synthesise the substances 5'-methoxyhydnocarpin-D (5'-MHC-D) and pheophorbide a, which have no antimicrobial activity but inhibit the expression of multidrug resistant efflux pumps (MDRs) in Staphylococcus aureus and potentiate the action of berberine. The MDR pumps extrude synthetic and natural antimicrobials from bacterial cells. We searched for these compounds in Berberis aetnensis C. Presl. (Berberidaceae), an endemic plant of the volcano Mount Etna. This work confirms the presence of pheophorbide a and permits us to hypothesise the presence of 5'-MHC-D in leaf extracts. In fact, the activity of ciprofloxacin was improved when two chromatographic fractions isolated from leaf extracts were added. These results are indicative of the presence of MDR pump inhibitors. Moreover, crude extracts were tested on several micro-organisms and showed antimicrobial activity mainly against Gram-positive bacteria and yeasts.

Topics: Anti-Bacterial Agents; Bacterial Proteins; Berberine; Berberis; Candida; Chlorophyll; Ciprofloxacin; Drug Interactions; Flavonoids; Gram-Negative Bacteria; Gram-Positive Bacteria; Molecular Structure; Multidrug Resistance-Associated Proteins; Plant Extracts; Staphylococcus aureus

2003

5'-Methoxyhydnocarpin-D and pheophorbide A: Berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus.

Journal of natural products, 2000, Volume: 63, Issue:8

A new method of bioactivity-directed fractionation, based on multidrug resistant pump (MDR) inhibition in Staphylococcus aureus, was demonstrated. This resulted in the isolation, from berberine-containing Berberis species, of two compounds that are themselves devoid of S. aureus antibacterial activity, but that form potent synergistic couples with a subinhibitory concentration of berberine. The bacterial MDR pump inhibitors were identified as the flavonolignan 2 and the porphyrin 3. Another natural flavonolignan, silybin (8) from Silybum marianum, was also shown to be a bacterial MDR pump inhibitor.

Topics: Berberine; Biological Assay; Chlorophyll; Chromatography, Gel; Chromatography, Thin Layer; Colorado; Drug Resistance, Microbial; Drug Resistance, Multiple; Flavonoids; Gas Chromatography-Mass Spectrometry; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Plant Leaves; Plants, Medicinal; Spectrometry, Mass, Fast Atom Bombardment; Spectrophotometry, Ultraviolet; Staphylococcus aureus

2000