5-(n-4-chlorobenzyl)-n-(2--4--dimethyl)benzamil and ethylisopropylamiloride

5-(n-4-chlorobenzyl)-n-(2--4--dimethyl)benzamil has been researched along with ethylisopropylamiloride* in 1 studies

Other Studies

1 other study(ies) available for 5-(n-4-chlorobenzyl)-n-(2--4--dimethyl)benzamil and ethylisopropylamiloride

Article	Year
Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets. FEBS letters, 1987, Dec-10, Volume: 225, Issue:1-2 Recently it has been suggested [(1987) Nature 325, 456-458; (1987) FEBS Lett. 212, 123-126] that the activation of Na+/H+ exchange is a prerequisite for platelet aggregation and the development of the Ca2+ signal. As direct evidence for the role of the Na+/H+-exchange pathway the inhibition of the Ca2+ signal by EIPA, a specific inhibitor of Na+/H+ exchange, was offered. Here we demonstrate that low concentrations of EIPA (below 1 microM) completely block Na+/H+ exchange while EIPA inhibits aggregation or Ca2+ mobilization only in concentrations 100-times greater than 1 microM. Moreover, another amiloride analogue, CBDMB, developed to act predominantly on Na+/Ca2+ exchange, does not affect Na+/H+ exchange in platelets but blocks aggregation and Ca2+ mobilization. We conclude that while Na+/H+ exchange has a fundamental role in platelet functions it is not prerequisite for the development of Ca2+ signal and aggregation. Topics: Amiloride; Blood Platelets; Calcium; Carrier Proteins; Humans; Platelet Aggregation; Protons; Sodium; Sodium-Hydrogen Exchangers; Thrombin	1987

Article

Year

Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets.

FEBS letters, 1987, Dec-10, Volume: 225, Issue:1-2

Recently it has been suggested [(1987) Nature 325, 456-458; (1987) FEBS Lett. 212, 123-126] that the activation of Na+/H+ exchange is a prerequisite for platelet aggregation and the development of the Ca2+ signal. As direct evidence for the role of the Na+/H+-exchange pathway the inhibition of the Ca2+ signal by EIPA, a specific inhibitor of Na+/H+ exchange, was offered. Here we demonstrate that low concentrations of EIPA (below 1 microM) completely block Na+/H+ exchange while EIPA inhibits aggregation or Ca2+ mobilization only in concentrations 100-times greater than 1 microM. Moreover, another amiloride analogue, CBDMB, developed to act predominantly on Na+/Ca2+ exchange, does not affect Na+/H+ exchange in platelets but blocks aggregation and Ca2+ mobilization. We conclude that while Na+/H+ exchange has a fundamental role in platelet functions it is not prerequisite for the development of Ca2+ signal and aggregation.

Topics: Amiloride; Blood Platelets; Calcium; Carrier Proteins; Humans; Platelet Aggregation; Protons; Sodium; Sodium-Hydrogen Exchangers; Thrombin

1987