4-nitrocatechol and 2-aminophenol

4-nitrocatechol has been researched along with 2-aminophenol* in 2 studies

Other Studies

2 other study(ies) available for 4-nitrocatechol and 2-aminophenol

ArticleYear
Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor.
    Acta crystallographica. Section D, Biological crystallography, 2013, Volume: 69, Issue:Pt 1

    Dioxygen activation by nonhaem Fe(II) enzymes containing the 2-His-1-carboxylate facial triad has been extensively studied in recent years. Here, crystal structures of 2-aminophenol 1,6-dioxygenase, an enzyme that represents a minor group of extradiol dioxygenases and that catalyses the ring opening of 2-aminophenol, in complex with the lactone intermediate (4Z,6Z)-3-iminooxepin-2(3H)-one and the product 2-aminomuconic 6-semialdehyde and in complex with the suicide inhibitor 4-nitrocatechol are reported. The Fe-ligand binding schemes observed in these structures revealed some common geometrical characteristics that are shared by the published structures of extradiol dioxygenases, suggesting that enzymes that catalyse the oxidation of noncatecholic compounds are very likely to utilize a similar strategy for dioxygen activation and the fission of aromatic rings as the canonical mechanism. The Fe-ligation arrangement, however, is strikingly enantiomeric to that of all other 2-His-1-carboxylate enzymes apart from protocatechuate 4,5-dioxygenase. This structural variance leads to the generation of an uncommon O(-)-Fe(2+)-O(-) species prior to O(2) binding, which probably forms the structural basis on which APD distinguishes its specific substrate and inhibitor, which share an analogous molecular structure.

    Topics: Aminophenols; Bacterial Proteins; Catalytic Domain; Catechols; Comamonas; Crystallization; Crystallography, X-Ray; Dioxygenases; Evolution, Molecular; Iron; Iron Deficiencies; Protein Subunits; Substrate Specificity

2013
Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor.
    Acta crystallographica. Section F, Structural biology and crystallization communications, 2012, Nov-01, Volume: 68, Issue:Pt 11

    Dioxygen activation implemented by nonhaem Fe(II) enzymes containing the 2-His-1-carboxylate facial triad has been extensively studied in recent years. Extradiol dioxygenase is the archetypal member of this superfamily and catalyzes the oxygenolytic ring opening of catechol analogues. Here, the crystallization and preliminary X-ray analysis of 2-aminophenol 1,6-dioxygenase, an enzyme representing a minor subset of extradiol dioxygenases that catalyze the fission of 2-aminophenol rather than catecholic compounds, is reported. Crystals of the holoenzyme with FeII and of complexes with the substrate 2-aminophenol and the suicide inhibitor 4-nitrocatechol were grown using the cocrystallization method under the same conditions as used for the crystallization of the apoenzyme. The crystals belonged to space group C2 and diffracted to 2.3-2.7 Å resolution; the crystal that diffracted to the highest resolution had unit-cell parameters a=270.24, b=48.39, c=108.55 Å, β=109.57°. All X-ray data sets collected from diffraction-quality crystals were suitable for structure determination.

    Topics: Aminophenols; Apoenzymes; Bacterial Proteins; Catechols; Chromatography, Gel; Chromatography, Ion Exchange; Comamonas testosteroni; Crystallization; Crystallography, X-Ray; Dioxygenases; Enzyme Inhibitors

2012