4-nitrocatechol and 1-3-benzodioxole

4-nitrocatechol has been researched along with 1-3-benzodioxole* in 1 studies

Other Studies

1 other study(ies) available for 4-nitrocatechol and 1-3-benzodioxole

ArticleYear
A spectrophotometric assay for the detection of fungal peroxygenases.
    Analytical biochemistry, 2012, Feb-01, Volume: 421, Issue:1

    Rapid and simple spectrophotometric methods are required for the unambiguous detection of recently discovered fungal peroxygenases in vivo and in vitro. This paper describes a peroxygenase-specific assay using 5-nitro-1,3-benzodioxole as substrate. The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (ε(425)=9,700 M(-1) cm(-1)), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514 nm (ε(514)=11,400 M(-1) cm(-1)). The assay is suitable for detecting peroxygenase activities in complex growth media and environmental samples as well as for high-throughput screenings.

    Topics: Catechols; Dioxoles; Fungi; High-Throughput Screening Assays; Hydrogen-Ion Concentration; Mixed Function Oxygenases; Spectrophotometry

2012