4-hydroxyquinazoline and hexamethylene-bisacetamide

Hexamethylenebisacetamide (HMBA) selectively and reversibly inhibited proliferation of human and rat vascular smooth-muscle cells (VSMCs) compared with endothelial cells, fibroblasts or lymphocytes. Half-maximal inhibition of VSMC proliferation occurred at 2-5 mM-HMBA, and at 30- greater than 50 mM for other cell types. HMBA also prevented de-differentiation, defined by the loss of smooth-muscle-specific myosin heavy chain, of primary rat VSMCs and caused partial re-differentiation of subcultured cells. Other inhibitors of ADP-ribosyltransferase were also selective inhibitors of VSMC proliferation.

Topics: Acetamides; Animals; Cell Division; Cells, Cultured; Coumarins; DNA Replication; Endothelium, Vascular; Fibroblasts; Hematinics; Humans; Kinetics; Muscle, Smooth, Vascular; Quinazolines; Quinazolinones; Rats; Thymidine