4-hydroxy-2-nonenal has been researched along with cupric-chloride* in 2 studies
2 other study(ies) available for 4-hydroxy-2-nonenal and cupric-chloride
| Article | Year |
|---|---|
Detection of new epitopes formed upon oxidation of low-density lipoprotein, lipoprotein (a) and very-low-density lipoprotein. Use of an antiserum against 4-hydroxynonenal-modified low-density lipoprotein.
4-Hydroxynonenal (HNE) is a major aldehydic propagation product formed during peroxidation of unsaturated fatty acids. The aldehyde was used to modify freshly prepared human low-density lipoprotein (LDL). A polyclonal antiserum was raised in the rabbit and absorbed with freshly prepared LDL. The antiserum did not react with human LDL, but reacted with CuCl2-oxidized LDL and in a dose-dependent manner with LDL, modified with 1, 2 and 3 mM-HNE, in the double-diffusion analysis. LDL treated with 4 mM of hexanal or hepta-2,4-dienal or 4-hydroxyhexenal or malonaldehyde (4 or 20 mM) did not react with the antiserum. However, LDL modified with 4 mM-4-hydroxyoctenal showed a very weak reaction. Lipoprotein (a) and very-low-density lipoprotein were revealed for the first time to undergo oxidative modification initiated by CuCl2. This was evidenced by the generation of lipid hydroperoxides and thiobarbituric acid-reactive substances, as well as by a marked increase in the electrophoretic mobility. After oxidation these two lipoproteins also reacted positively with the antiserum against HNE-modified LDL. Topics: Adult; Aldehydes; Copper; Epitopes; Female; Humans; Immune Sera; Immunodiffusion; Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Lipoproteins, VLDL; Male; Oxidation-Reduction | 1990 |
Oxidation of human low density lipoprotein initiated by copper (II) chloride.
Topics: Aldehydes; beta Carotene; Carotenoids; Copper; Free Radicals; Humans; Kinetics; Lipoproteins, LDL; Oxidation-Reduction; Vitamin E | 1988 |