4-hydroxy-2-nonenal and 3-hydroxy-naphthoic-acid-hydrazide

4-hydroxy-2-nonenal has been researched along with 3-hydroxy-naphthoic-acid-hydrazide* in 2 studies

Other Studies

2 other study(ies) available for 4-hydroxy-2-nonenal and 3-hydroxy-naphthoic-acid-hydrazide

Article	Year
Imaging of oxidative stress at subcellular level by confocal laser scanning microscopy after fluorescent derivatization of cellular carbonyls. The American journal of pathology, 1993, Volume: 142, Issue:5 Confocal laser scanning fluorescence microscopy plus image videoanalysis was used to visualize the tissue areas and the subcellular sites first involved by oxidative stress and lipid peroxidation, in the well-established experimental model of lipid peroxidation induced by haloalkane intoxication in the liver cell. The fluorescent reagent 3-hydroxy-2-naphthoic acid hydrazide was employed to derivativize the carbonyl functions originating from the lipoperoxidative process in situ, in liver cryostat sections from in vivo intoxicated rats, as well as in isolated hepatocytes exposed in vitro to the pro-oxidant action of haloalkanes. The results obtained indicate that: 1) the detection of fluorescent derivatives of carbonyls indeed offers a gain in sensitivity, 2) haloalkane-induced lipid peroxidation in hepatocytes primarily involves the perinuclear endoplasmic reticulum, whereas the plasma membrane and the nuclear compartment are unaffected, and 3) lipid peroxidation also induces an increase of liver autofluorescence. Topics: Aldehydes; Amidines; Animals; Carbon Tetrachloride; Fluorescent Dyes; Image Processing, Computer-Assisted; Lasers; Lipid Peroxides; Liver; Male; Microscopy; Microscopy, Fluorescence; Naphthols; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Subcellular Fractions	1993
Quantification of the histochemical staining for carbonyles and DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B. Histochemistry, 1990, Volume: 94, Issue:5 Fixed cells and tissues pretreated with 4-hydroxynonenal were used as models for the histochemical demonstration of protein bound aldehydic groups. The aldehydes were stained with both a modification of the 2,4-dinitrophenylhydrazine method (2,4-DNPH) and the optimized staining using 3-hydroxy-2-naphthoic acid hydrazide and Fast blue B (NAH-FB). A correlation has been found between the specific microphotometric mean integrated maximum absorbance values of cells and tissues stained with 2,4-DNPH and with NAH-FB (cc = 0.999). The maximum absorbance measured after 2,4-DNPH-staining (epsilon 367 = 21,000) were 1.893 +/- 0.072 (P less than 0.01) times that of NAH-FB-staining at 550 nm. Microphotometrically determined DNA-values of different cells stained with the NAH-FB-DNA-method correlated with those determined with methods of analytical biochemistry and published by other authors. Topics: Aldehydes; Animals; Carcinoma, Ehrlich Tumor; Diazonium Compounds; DNA; Histocytochemistry; Liver; Microchemistry; Naphthols; Phenylhydrazines; Photometry; Proteins; Rats	1990

Article

Year

Imaging of oxidative stress at subcellular level by confocal laser scanning microscopy after fluorescent derivatization of cellular carbonyls.

The American journal of pathology, 1993, Volume: 142, Issue:5

Confocal laser scanning fluorescence microscopy plus image videoanalysis was used to visualize the tissue areas and the subcellular sites first involved by oxidative stress and lipid peroxidation, in the well-established experimental model of lipid peroxidation induced by haloalkane intoxication in the liver cell. The fluorescent reagent 3-hydroxy-2-naphthoic acid hydrazide was employed to derivativize the carbonyl functions originating from the lipoperoxidative process in situ, in liver cryostat sections from in vivo intoxicated rats, as well as in isolated hepatocytes exposed in vitro to the pro-oxidant action of haloalkanes. The results obtained indicate that: 1) the detection of fluorescent derivatives of carbonyls indeed offers a gain in sensitivity, 2) haloalkane-induced lipid peroxidation in hepatocytes primarily involves the perinuclear endoplasmic reticulum, whereas the plasma membrane and the nuclear compartment are unaffected, and 3) lipid peroxidation also induces an increase of liver autofluorescence.

Topics: Aldehydes; Amidines; Animals; Carbon Tetrachloride; Fluorescent Dyes; Image Processing, Computer-Assisted; Lasers; Lipid Peroxides; Liver; Male; Microscopy; Microscopy, Fluorescence; Naphthols; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Subcellular Fractions

1993

Quantification of the histochemical staining for carbonyles and DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B.

Histochemistry, 1990, Volume: 94, Issue:5

Fixed cells and tissues pretreated with 4-hydroxynonenal were used as models for the histochemical demonstration of protein bound aldehydic groups. The aldehydes were stained with both a modification of the 2,4-dinitrophenylhydrazine method (2,4-DNPH) and the optimized staining using 3-hydroxy-2-naphthoic acid hydrazide and Fast blue B (NAH-FB). A correlation has been found between the specific microphotometric mean integrated maximum absorbance values of cells and tissues stained with 2,4-DNPH and with NAH-FB (cc = 0.999). The maximum absorbance measured after 2,4-DNPH-staining (epsilon 367 = 21,000) were 1.893 +/- 0.072 (P less than 0.01) times that of NAH-FB-staining at 550 nm. Microphotometrically determined DNA-values of different cells stained with the NAH-FB-DNA-method correlated with those determined with methods of analytical biochemistry and published by other authors.

Topics: Aldehydes; Animals; Carcinoma, Ehrlich Tumor; Diazonium Compounds; DNA; Histocytochemistry; Liver; Microchemistry; Naphthols; Phenylhydrazines; Photometry; Proteins; Rats

1990