4-demethylwyosine and 1-methylguanosine

4-demethylwyosine has been researched along with 1-methylguanosine* in 1 studies

Other Studies

1 other study(ies) available for 4-demethylwyosine and 1-methylguanosine

Article	Year
Mechanistic Studies of the Radical S-Adenosyl-L-methionine Enzyme 4-Demethylwyosine Synthase Reveal the Site of Hydrogen Atom Abstraction. Biochemistry, 2015, Jun-16, Volume: 54, Issue:23 TYW1 catalyzes the formation of 4-demethylwyosine via the condensation of N-methylguanosine (m¹G) with carbons 2 and 3 of pyruvate. In this study, labeled transfer ribonucleic acid (tRNA) and pyruvate were utilized to determine the site of hydrogen atom abstraction and regiochemistry of the pyruvate addition. tRNA containing a ²H-labeled m¹G methyl group was used to identify the methyl group of m¹G as the site of hydrogen atom abstraction by 5'-deoxyadenosyl radical. [2-¹³C₁-3,3,3-²H₃]Pyruvate was used to demonstrate retention of all the pyruvate protons, indicating that C2 of pyruvate forms the bridging carbon of the imidazoline ring and C3 the methyl. Topics: Archaeal Proteins; Biocatalysis; Carbon Radioisotopes; Carboxy-Lyases; Catalytic Domain; Deuterium; Free Radicals; Guanosine; Iron-Sulfur Proteins; Methanococcus; Methylation; Models, Molecular; Pyruvic Acid; RNA, Archaeal; RNA, Transfer, Phe; S-Adenosylmethionine; Stereoisomerism	2015

Article

Year

Mechanistic Studies of the Radical S-Adenosyl-L-methionine Enzyme 4-Demethylwyosine Synthase Reveal the Site of Hydrogen Atom Abstraction.

Biochemistry, 2015, Jun-16, Volume: 54, Issue:23

TYW1 catalyzes the formation of 4-demethylwyosine via the condensation of N-methylguanosine (m¹G) with carbons 2 and 3 of pyruvate. In this study, labeled transfer ribonucleic acid (tRNA) and pyruvate were utilized to determine the site of hydrogen atom abstraction and regiochemistry of the pyruvate addition. tRNA containing a ²H-labeled m¹G methyl group was used to identify the methyl group of m¹G as the site of hydrogen atom abstraction by 5'-deoxyadenosyl radical. [2-¹³C₁-3,3,3-²H₃]Pyruvate was used to demonstrate retention of all the pyruvate protons, indicating that C2 of pyruvate forms the bridging carbon of the imidazoline ring and C3 the methyl.

Topics: Archaeal Proteins; Biocatalysis; Carbon Radioisotopes; Carboxy-Lyases; Catalytic Domain; Deuterium; Free Radicals; Guanosine; Iron-Sulfur Proteins; Methanococcus; Methylation; Models, Molecular; Pyruvic Acid; RNA, Archaeal; RNA, Transfer, Phe; S-Adenosylmethionine; Stereoisomerism

2015