4-amino-5-hydrazino-1-2-4-triazole-3-thiol has been researched along with tricine* in 1 studies
1 review(s) available for 4-amino-5-hydrazino-1-2-4-triazole-3-thiol and tricine
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Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.
Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria. Topics: Bacteriological Techniques; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Glycine; Lipid A; Lipopolysaccharides; Molecular Probe Techniques; O Antigens; Silver Staining; Sugar Acids; Sulfhydryl Compounds | 2006 |