Compounds > 4-acetamido-4--isothiocyanatostilbene-2-2--disulfonic-acid

4-acetamido-4--isothiocyanatostilbene-2-2--disulfonic-acid and gadolinium-chloride

4-acetamido-4--isothiocyanatostilbene-2-2--disulfonic-acid has been researched along with gadolinium-chloride* in 2 studies

Other Studies

2 other study(ies) available for 4-acetamido-4--isothiocyanatostilbene-2-2--disulfonic-acid and gadolinium-chloride

Article	Year
Role of potassium and chlorine channels in the regulation of thymocyte volume in rats. Bulletin of experimental biology and medicine, 2008, Volume: 145, Issue:5 Regulatory decrease in thymocyte volume under conditions of osmotic stress was abolished by potassium and chlorine channel blockers. Osmotic stress-activated chlorine channels belong to 2 pharmacological types. The maxi-anion channel is sensitive to Gd(3+). The volume-sensitive outwardly rectifying chlorine channel is inhibited with glybenclamide and phloretin. Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Animals; Cell Size; Chloride Channels; Gadolinium; Glyburide; In Vitro Techniques; Ion Transport; Osmotic Pressure; Phloretin; Potassium Channel Blockers; Potassium Channels; Rats; Rats, Wistar; T-Lymphocytes	2008
Electrophysiological, mechanosensitive responses of Xenopus laevis oocytes to direct, isotonic increase in intracellular volume. Journal of neuroscience methods, 2003, May-30, Volume: 125, Issue:1-2 An intra-oocyte injection method for obtaining the electrophysiological response of follicle-enclosed Xenopus laevis oocytes to an increase in intracellular volume (i.e. stretch) without changing the extracellular medium is described. The response comprised a 'stretch-activated' (SA) current which was evoked by injection of an isotonic 14-70 nl droplet and had a transient, smooth profile. Ionic substitution experiments revealed that the current was carried mainly by Na(+), K(+) and Cl(-) and had a reversal potential of about -2 mV. A similar result was obtained from experiments in which the holding potential was varied between -40 and +10 mV whilst repeatedly inducing the SA current. On average, the channel was blocked 60% by 10 microM gadolinium chloride, 58% by 50 microM amiloride, 11% by 50 microM 4,4'-diisothiocyanatostilbene-2, 2'-disulfonic acid and 63% by 50 microM 4-acetamido-4'-isothiocyanato-stilbene-2-2'-disulfonic acid. Maturation of the oocytes with 100 microM progesterone reduced the mechanosensitivity 12-fold. This injection technique is compared with other methods of eliciting mechanosensitive (MS) currents in X. laevis oocytes. These observed characteristics of the SA current are discussed in relation to the oocytes' endogenous MS cation and anion channels. Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Amiloride; Animals; Anti-Inflammatory Agents; Cell Size; Choline; Diuretics; Dose-Response Relationship, Drug; Electric Conductivity; Electrophysiology; Female; Gadolinium; Intracellular Fluid; Isotonic Solutions; Magnesium; Mechanotransduction, Cellular; Membrane Potentials; Oocytes; Patch-Clamp Techniques; Progesterone; Sodium; Time Factors; Xenopus laevis	2003

Article

Year

Role of potassium and chlorine channels in the regulation of thymocyte volume in rats.

Bulletin of experimental biology and medicine, 2008, Volume: 145, Issue:5

Regulatory decrease in thymocyte volume under conditions of osmotic stress was abolished by potassium and chlorine channel blockers. Osmotic stress-activated chlorine channels belong to 2 pharmacological types. The maxi-anion channel is sensitive to Gd(3+). The volume-sensitive outwardly rectifying chlorine channel is inhibited with glybenclamide and phloretin.

Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Animals; Cell Size; Chloride Channels; Gadolinium; Glyburide; In Vitro Techniques; Ion Transport; Osmotic Pressure; Phloretin; Potassium Channel Blockers; Potassium Channels; Rats; Rats, Wistar; T-Lymphocytes

2008

Electrophysiological, mechanosensitive responses of Xenopus laevis oocytes to direct, isotonic increase in intracellular volume.

Journal of neuroscience methods, 2003, May-30, Volume: 125, Issue:1-2

An intra-oocyte injection method for obtaining the electrophysiological response of follicle-enclosed Xenopus laevis oocytes to an increase in intracellular volume (i.e. stretch) without changing the extracellular medium is described. The response comprised a 'stretch-activated' (SA) current which was evoked by injection of an isotonic 14-70 nl droplet and had a transient, smooth profile. Ionic substitution experiments revealed that the current was carried mainly by Na(+), K(+) and Cl(-) and had a reversal potential of about -2 mV. A similar result was obtained from experiments in which the holding potential was varied between -40 and +10 mV whilst repeatedly inducing the SA current. On average, the channel was blocked 60% by 10 microM gadolinium chloride, 58% by 50 microM amiloride, 11% by 50 microM 4,4'-diisothiocyanatostilbene-2, 2'-disulfonic acid and 63% by 50 microM 4-acetamido-4'-isothiocyanato-stilbene-2-2'-disulfonic acid. Maturation of the oocytes with 100 microM progesterone reduced the mechanosensitivity 12-fold. This injection technique is compared with other methods of eliciting mechanosensitive (MS) currents in X. laevis oocytes. These observed characteristics of the SA current are discussed in relation to the oocytes' endogenous MS cation and anion channels.

Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Amiloride; Animals; Anti-Inflammatory Agents; Cell Size; Choline; Diuretics; Dose-Response Relationship, Drug; Electric Conductivity; Electrophysiology; Female; Gadolinium; Intracellular Fluid; Isotonic Solutions; Magnesium; Mechanotransduction, Cellular; Membrane Potentials; Oocytes; Patch-Clamp Techniques; Progesterone; Sodium; Time Factors; Xenopus laevis

2003