4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and triphenylphosphine

A mitochondria-targetable probe Mito-DHP for nitric oxide (NO) was designed and synthesized by introducing dihydropyridine and triphenylphosphonium (TPP) moieties into boron dipyrromethene (BODIPY) dye. Mito-DHP was able to effectively detect nitric oxide through the aromatization of dihydropyridine to fluorescent pyridine product under oxygen-free conditions. The probe Mito-DHP showed high selectivity to NO over a number of reactive oxygen/nitrogen species (ROS/RNS) as well as high sensitivity (detection limit at 25nM), pH stability and bio-compatibility. Furthermore, Mito-DHP proved to target mitochondria specifically and to visualize both exogenous and endogenous NO in real time.

Topics: Animals; Boron Compounds; Cell Survival; Dihydropyridines; Fluorescent Dyes; Hep G2 Cells; Humans; Mice; Mitochondria; Nitric Oxide; Organophosphorus Compounds; RAW 264.7 Cells