4-4-difluoro-4-bora-3a-4a-diaza-s-indacene has been researched along with ethidium-homodimer* in 1 studies
1 other study(ies) available for 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and ethidium-homodimer
| Article | Year |
|---|---|
Analysis of the structure and composition of individual lipoplex particles by flow fluorometry.
A flow fluorometric approach to study cationic lipoid-DNA complexes is presented. The approach uses standard flow cytometry equipment and common fluorescent dyes (BODIPY and ethidium homodimer-2) to detect both lipoid and DNA content in individual particles. In addition, a procedure that allows determination of whether or not liposomes remain intact is described. The procedure is based on monitoring the retention of a polar tracer that has been preloaded into its aqueous compartment. Sample preparation, instrument setup, data analysis, and methodological limitations are described. Applications of the procedure to cationic lipoid-DNA complexes are described, and illustrations are given for the determination of how the lipoid content, composition, and structure of individual lipoplexes in a population evolve over time, starting at about 1 min after DNA and vesicles are mixed. Analogous procedures can be applied to other heterogeneous particles and supramolecular structures. Topics: Boron Compounds; Cations; DNA; Ethidium; Flow Cytometry; Fluorescent Dyes; Lipids; Liposomes | 2005 |