4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and deoxy-5-methylcytidylic-acid

4-4-difluoro-4-bora-3a-4a-diaza-s-indacene has been researched along with deoxy-5-methylcytidylic-acid* in 1 studies

Other Studies

1 other study(ies) available for 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and deoxy-5-methylcytidylic-acid

Article	Year
Determination of the DNA methylation level in tumor cells by capillary electrophoresis and laser-induced fluorescence detection. Electrophoresis, 2004, Volume: 25, Issue:6 An analytical method to determine the genome-wide DNA methylation in only 100 ng DNA is presented. The analysis is based on DNA isolation and hydrolysis followed by derivatization of the 2'-desoxyribonucleoside-3'-monophosphates with a fluorescence dye (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylene diamine hydrochloride, Bodipy FL EDA). The separation of the derivatives was carried out by micellar electrokinetic chromatography, and laser-induced fluorescence was used for detection. To calculate the methylation level, the derivatization factor and the quantum yields of the Bodipy conjugates of 2'-desoxycytidine-3'-monophosphate (dCMP) and 2'-desoxy-5-methylcytidine-3'-monophosphate (5m-dCMP) were determined by measurement of methylated Lambda DNA. The assignment was made by cochromatography with the synthesized and characterized standard compound 5m-dCMP. After optimization of the method it was possible to determine the methylation level in 100-ng DNA samples with a standard deviation of less than 5%. Topics: Bacteriophage lambda; Boron Compounds; Breast Neoplasms; Cell Line; Deoxycytidine Monophosphate; DNA Methylation; DNA, Neoplasm; Electrophoresis, Capillary; Fluorescent Dyes; Humans; Lasers; Neoplasms	2004

Article

Year

Determination of the DNA methylation level in tumor cells by capillary electrophoresis and laser-induced fluorescence detection.

Electrophoresis, 2004, Volume: 25, Issue:6

An analytical method to determine the genome-wide DNA methylation in only 100 ng DNA is presented. The analysis is based on DNA isolation and hydrolysis followed by derivatization of the 2'-desoxyribonucleoside-3'-monophosphates with a fluorescence dye (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylene diamine hydrochloride, Bodipy FL EDA). The separation of the derivatives was carried out by micellar electrokinetic chromatography, and laser-induced fluorescence was used for detection. To calculate the methylation level, the derivatization factor and the quantum yields of the Bodipy conjugates of 2'-desoxycytidine-3'-monophosphate (dCMP) and 2'-desoxy-5-methylcytidine-3'-monophosphate (5m-dCMP) were determined by measurement of methylated Lambda DNA. The assignment was made by cochromatography with the synthesized and characterized standard compound 5m-dCMP. After optimization of the method it was possible to determine the methylation level in 100-ng DNA samples with a standard deviation of less than 5%.

Topics: Bacteriophage lambda; Boron Compounds; Breast Neoplasms; Cell Line; Deoxycytidine Monophosphate; DNA Methylation; DNA, Neoplasm; Electrophoresis, Capillary; Fluorescent Dyes; Humans; Lasers; Neoplasms

2004