3-methylpseudouridine has been researched along with 1-methylpseudouridine* in 1 studies
1 other study(ies) available for 3-methylpseudouridine and 1-methylpseudouridine
| Article | Year |
|---|---|
The isolation from ribonucleic acid of substituted uridines containing alpha-aminobutyrate moieties derived from methionine.
The RNA of an established line of Chinese hamster cells growing in cell culture contains a small number of uridines substituted at the 3-position with a gamma-linked alpha-aminobutyrate residue. Structure has been ascertained by: (a) examination of incorporation of isotopic labels from precursors; (b) degradation with anhydrous hydrazine and comparison of the products with synthetic material or with hydrazinolysis products of known uridines; and (c) comparison of the unknowns as their hydantoin derivatives with the 5-beta-(bromoethyl)hydantoin alkylation products of uridine and of 1- and 3-methylpseudouridine. In this manner it is shown that 18-S RNA of ribosomes contains a single residue of a nucleoside which we tentatively identify as 1-methyl-3-gamma-(alpha-amino-alpha-carboxypropyl)pseudouridine per molecule. RNA isolated from the supernatant fraction, sedimenting at 4 S and co-electrophoresing with transfer RNA on polyacrylamide gels, contains several similar bases, one of which is identified as 3-gamma-(alpha-amino-alpha-carboxypropyl)uridine. Each of the above nucleosides derives its alpha-aminobutyrate residue from methionine. Topics: Aminobutyrates; Animals; Centrifugation, Density Gradient; CHO Cells; Chromatography, Paper; Cricetinae; Cricetulus; Hydralazine; Hydrogen-Ion Concentration; Methionine; Models, Chemical; Pseudouridine; RNA; Spectrophotometry; Tritium; Uridine | 1974 |