3-methyl-2-benzothiazolone-hydrazone and ferric-chloride

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at lambdamax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with lambdamax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 microg mL-1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

Topics: Anti-Bacterial Agents; Benzothiazoles; Ceftazidime; Chemistry, Pharmaceutical; Chlorides; Dosage Forms; Ethylenediamines; Excipients; Ferric Compounds; Hydrazones; Hydrogen-Ion Concentration; Injections; Reproducibility of Results; Spectrophotometry; Technology, Pharmaceutical

A sensitive spectrophotometric method for the determination of amitriptyline hydrochloride, nortriptyline hydrochloride and doxepin hydrochloride in pure and dosage forms, is described. The method is based on the oxidative coupling of the drugs with 3-methylbenzothiazolin-2-one hydrazone in the presence of iron(III) chloride in 1 M hydrochloric acid. The commonly encountered excipients and additives do not interfere with the determinations. Results of the present method are comparable with those of official methods. The new method offers the advantage of simplicity and rapidity.

Topics: Amitriptyline; Antidepressive Agents; Benzothiazoles; Chlorides; Doxepin; Excipients; Ferric Compounds; Hydrazones; Hydrochloric Acid; Nortriptyline; Spectrophotometry; Thiazoles; Time Factors