3-azidotyrosine has been researched along with triphenylphosphine* in 1 studies
1 other study(ies) available for 3-azidotyrosine and triphenylphosphine
Article | Year |
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Site-selective post-translational modification of proteins using an unnatural amino acid, 3-azidotyrosine.
An efficient method for site-selective modification of proteins using an unnatural amino acid, 3-azidotyrosine has been developed. This method utilizes the yeast amber suppressor tRNA(Tyr)/mutated tyrosyl-tRNA synthetase pair as a carrier of 3-azidotyrosine in an Escherichia coli cell-free translation system, and triarylphosphine derivatives for specific modification of the azido group. Using rat calmodulin (CaM) as a model protein, we prepared several unnatural CaM molecules, each carrying an azidotyrosine at predetermined positions 72, 78, 80 or 100, respectively. Post-translational modification of these proteins with a conjugate compound of triarylphosphine and biotin produced site-selectively biotinylated CaM molecules. Reaction efficiency was similar among these proteins irrespective of the position of introduction, and site-specificity of biotinylation was confirmed using mass spectrometry. In addition, CBP-binding activity of the biotinylated CaMs was confirmed to be similar to that of wild-type CaM. This method is intrinsically versatile in that it should be easily applicable to introducing any other desirable compounds (e.g., probes and cross-linkers) into selected sites of proteins as far as appropriate derivative compounds of triarylphosphine could be chemically synthesized. Elucidation of molecular mechanisms of protein functions and protein-to-protein networks will be greatly facilitated by making use of these site-selectively modified proteins. Topics: Amino Acid Sequence; Azides; Biotinylation; Calmodulin; Cell-Free System; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Organophosphorus Compounds; Protein Processing, Post-Translational; RNA, Transfer, Tyr; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tyrosine; Tyrosine-tRNA Ligase | 2007 |