Compounds > 3-amino-5-mercapto-1-2-4-triazole

3-amino-5-mercapto-1-2-4-triazole and acetonitrile

3-amino-5-mercapto-1-2-4-triazole has been researched along with acetonitrile* in 1 studies

Other Studies

1 other study(ies) available for 3-amino-5-mercapto-1-2-4-triazole and acetonitrile

ArticleYear
Determination of 3-amino-5-mercapto-1,2,4-triazole in serum.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2004, Mar-05, Volume: 801, Issue:2

    A high performance liquid chromatography (HPLC) method using fluorescence detection to determine 3-amino-5-mercapto-1,2,4-triazole (AMT) levels in serum has been developed. Sample preparation involved treatment with tributylphosphine (TBP) to reduce disulfides formed during storage, precipitation of proteins with acetonitrile (ACN), and precolumn derivatization using the thiol reactive fluorescent probe monobromobimane (MBB). The conjugate (AMT-MBB) was resolved by gradient elution from a C(18) reversed-phase column. The assay method was linear over a concentration range of 0.78-50 microg/ml and had a limit of detection (LOD) of 0.05 microg/ml AMT (10 microl injection). This method provides a sensitive and specific tool for the determination of AMT in serum and may have potential industrial hygiene application.

    Topics: Acetonitriles; Animals; Chemical Precipitation; Chromatography, High Pressure Liquid; Fluorescent Dyes; Humans; Male; Quality Control; Rats; Rats, Inbred F344; Sensitivity and Specificity; Solutions; Triazoles; Water

2004