3-7-12-trihydroxycholest-24-enoic-acid has been researched along with 3-7-12-trihydroxycholestan-26-oic-acid* in 2 studies
2 other study(ies) available for 3-7-12-trihydroxycholest-24-enoic-acid and 3-7-12-trihydroxycholestan-26-oic-acid
| Article | Year |
|---|---|
Non-stereoselective formation of 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 beta-cholestan-26-oic acid during cholic acid biosynthesis.
Incubation of (25RS)-, (25R)- and (25S)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA, 6, 6a, 6b) and (24E)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-en-26-oic acid (7) with rat liver mitochondria gave all four stereoisomers (9a,9b,9c,9d) of 3 alpha,7 alpha,12 alpha,24-Tetrahydroxy-5 beta-cholestan-26-oic acid (TeHCA). The corresponding 27-nor analogs (10,11) were also converted non-stereoselectively to a 1:1 mixture of the epimeric 24-hydroxy compounds (12). Topics: Animals; Cholestanols; Cholesterol; Cholic Acid; Cholic Acids; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Male; Mitochondria, Liver; Rats; Rats, Wistar; Stereoisomerism | 1994 |
Identification of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholest-24-enoic acid as an intermediate in the peroxisomal conversion of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid to cholic acid.
In this study, we have identified a delta 24-unsaturated intermediate involved in the conversion of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid to cholic acid by the peroxisomal fraction of rat liver. An accumulation of this intermediate was observed when NAD+ was omitted from the reaction mixture. The intermediate was isolated by reversed-phase high-pressure liquid chromatography and identified by combined gas-liquid chromatography-mass spectrometry. The peroxisomal fraction was able to catalyze the conversion of the delta 24-unsaturated intermediate to cholic acid in the presence of CoA, ATP, Mg2+ and NAD+. The identification of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-enoic acid in cholic acid formation supports the proposed reaction mechanism in which the side-chain cleavage of C27-steroids is similar to that of peroxisomal beta-oxidation of fatty acids. This involves an FAD-dependent oxidase acting on 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-CoA. Topics: Adenosine Triphosphate; Animals; Cholestanols; Cholesterol; Cholic Acid; Cholic Acids; Chromatography, High Pressure Liquid; Coenzyme A; Gas Chromatography-Mass Spectrometry; Liver; Magnesium; Male; Microbodies; NAD; Rats; Rats, Inbred Strains | 1989 |