3-4-dihydroxyphenylpropionic-acid and hippuric-acid

The separation and detection of 11 urinary aromatic acids was developed using HPLC-MS/MS. The method features a simple sample preparation involving a single-step dilution with internal standard and a rapid 8 min chromatographic separation. The accuracy was evaluated by the recovery of known spikes between 87 and 110%. Inter- and intra-assay precision (CV) was below 11% in all cases and the analytes were observed to be stable for up to 8 weeks when stored at -20 degrees C. The method was validated based upon linearity, accuracy, precision and stability and was used to establish reference intervals for children and adults.

Topics: Acids, Heterocyclic; Adult; Caffeic Acids; Carboxylic Acids; Child; Chromatography, Liquid; Hippurates; Homovanillic Acid; Humans; Kynurenic Acid; Parabens; Phenylacetates; Reproducibility of Results; Tandem Mass Spectrometry; Vanilmandelic Acid; Xanthurenates