3-4-dihydroxyphenyllactic-acid and puerarin

Topics: Benzyl Alcohols; Chlorogenic Acid; Chromatography, Liquid; Drugs, Chinese Herbal; Flavonoids; Glucosides; Glycyrrhizic Acid; Ion Mobility Spectrometry; Isoflavones; Lactates; Limit of Detection; Mass Spectrometry; Rutin; Spectrometry, Mass, Electrospray Ionization

Ischemic heart diseases are the leading cause of death in both developed and developing countries over the past decades. The aim of this study was to investigate the cardioprotective effects of the complex preparation (called Shenge), made of puerarin and Danshensu, on acute ischemic myocardial injury in rats and its underlying mechanisms. The left anterior descending (LAD) coronary artery was occluded to induce myocardial ischemia in hearts of SD rats. Shenge was injected into the tail vein 15 min after occlusion at doses of 0, 30, 60 or 120 mg/kg. Then, the ST elevation was measured at 60, 120 and 240 min after Shenge administration. The infarct size, serum levels of creatine kinase isoenzyme-MB (CK-MB), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA), and the ST elevation were measured after the rats were killed. Shenge decreased the ST elevation induced by acute myocardial ischemia, reduced infarct size, serum levels of CK-MB, LDH and MDA and increased the serum activity of SOD in a dose-dependent manner. The combined use of puerarin and Danshensu at a ratio of 1:1 shows the most effective activity. In conclusion, Shenge exerts significant cardioprotective effects against acute ischemic myocardial injury in rats, likely through its antioxidant and antilipid peroxidation properties, and thus may be used as an effective and promising medicine for both prophylaxis and treatment of ischemic heart disease.

Topics: Animals; Cardiotonic Agents; Diltiazem; Dose-Response Relationship, Drug; Drug Combinations; Isoflavones; Lactates; Molecular Conformation; Myocardial Ischemia; Rats; Rats, Sprague-Dawley; Time Factors; Vasodilator Agents

Ischemic heart diseases have been the leading cause of death in both developed and developing countries over the past decades. The aim of this study was to investigate the cardioprotective effects of the complex preparation (called Shenge), made of puerarin (isolated from Pueraria lobata Ohwi., also called Kudzu) and Danshensu (isolated from the Chinese herb, Salvia miltiorrhiza), on acute ischemic myocardial injury in rats and its underlying mechanisms. The left anterior descending (LAD) coronary artery was occluded to induce myocardial ischemia in the hearts of SD rats. Shenge was injected into the tail vein 15 min after occlusion at doses of 0, 30, 60, or 120 mg/kg body wt. ST elevation was then measured at 60, 120, and 240 min after Shenge administration. The ischemic size, serum levels of creatine kinase isoenzyme-MB (CK-MB), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA), and ST elevation were measured after the rats were sacrificed. Shenge decreased ST elevation induced by acute myocardial ischemia, reduced ischemic size, serum levels of CK-MB, LDH and MDA, and increased serum activity of SOD in a dose-dependent manner. The combined use of puerarin and Danshensu at a ratio of 1:1 showed the most effective activity. In conclusion, Shenge exerts significant cardioprotective effects against acute ischemic myocardial injury in rats, likely through its antioxidant and anti-lipid peroxidation properties, and thus may be an effective and promising medicine for both prophylaxis and treatment of ischemic heart disease.

Topics: Animals; Cardiotonic Agents; Coronary Circulation; Disease Models, Animal; Isoflavones; Lactates; Male; Myocardial Reperfusion Injury; Phytotherapy; Plant Extracts; Pueraria; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza

To predict the stable life for a complex injection of Chinese materia medica.. The prediction was carried out by initial average rate stability tests, and the contents of puerarin and danshensu (major active components of the injection) were assayed by HPLC.. The degradation of puerarin followed the first order action, while danshensu was hard to determine; the t0.9,25 degrees C was 3.49 years and 1.69 years respectively.. The stable life of the injection has been determined as 1.5 years.

Topics: Drug Combinations; Drug Stability; Drugs, Chinese Herbal; Injections; Isoflavones; Lactates; Plants, Medicinal; Pueraria; Salvia miltiorrhiza

The viscosity of ethanol-water at various temperatures was determined. The physicochemical properties and characters of the solvents used as the mobile phase of reversed-phase high performance liquid chromatography(RP-HPLC) were compared between methanol and ethanol. The components of several Chinese medicines have been determined by RP-HPLC with ethanol-water used as mobile phase, and the results were compared with those obtained with methanol-water or acetonitrile-water. It showed that ethanol can be used as RP-HPLC mobile phase by increasing the column temperature moderately.

Topics: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Ethanol; Flavonoids; Isoflavones; Lactates; Methanol; Water