3-4-dihydroxybenzalacetone and caffeic-acid-phenethyl-ester

3,4-dihydroxybenzalacetone (DBL) and Caffeic acid phenethyl ester (CAPE) are both catechol-containing phenylpropanoid derivatives with diverse bioactivities. In the present study, we analyzed the ability of these compounds to activate the unfolded protein response (UPR) and the oxidative stress response. When human SH-SY5Y neuroblastoma cells were treated with DBL or CAPE, the expression of endoplasmic reticulum (ER) stress-related genes such as HSPA5, HYOU1, DDIT3, and SEC61b increased to a larger extent in response to CAPE treatment, while that of antioxidant genes such as HMOX1, GCLM, and NQO1 increased to a larger extent in response to DBL treatment. DNA microarray analysis confirmed the strong link of these compounds to ER stress. Regarding the mechanism, activation of the UPR by these compounds was associated with enhanced levels of oxidized proteins in the ER, and N-acetyl cysteine (NAC), which provides anti-oxidative effects, suppressed the induction of the UPR-target genes. Furthermore, both compounds enhanced the expression of LC3-II, a marker of autophagy, and 4-Phenylbutyric acid (4-PBA), a chemical chaperone that reduces ER stress, suppressed it. Finally, pretreatment of cells with DBL, CAPE or low doses of ER stressors protected cells against a neurotoxin 6-hydroxydopamine (6-OHDA) in an autophagy-dependent manner. These results suggest that DBL and CAPE induce oxidized protein-mediated ER stress and autophagy that may have a preconditioning effect in SH-SY5Y cells.

Topics: Autophagy; Caffeic Acids; Cell Line, Tumor; Dose-Response Relationship, Drug; Endoplasmic Reticulum Chaperone BiP; Endoplasmic Reticulum Stress; Gene Expression Regulation; Humans; Microtubule-Associated Proteins; Neurons; Neuroprotective Agents; Oxidative Stress; Oxidopamine; Phenylethyl Alcohol; Signal Transduction; Time Factors; Unfolded Protein Response

3,4-Dihydroxybenzalacetone (DBL) and caffeic acid phenethyl ester (CAPE) are both catechol-containing phenylpropanoid derivatives with various bioactivities. In the present study, we compared the effects of these compounds and other phenylpropanoid derivatives on the activation of nuclear factor-κB (NF-κB) signaling, a major pathway in the inflammatory response, using RAW 264.7 cells. Lipopolysaccharide (LPS)- and interferon γ-induced production of nitrite was strongly suppressed by CAPE and, to a lesser extent, by DBL and caffeic acid ethyl ester. Consistent with these results, induction of NF-κB downstream genes, such as Nitric oxide synthase, interleukin 1 beta, and interleukin 6, and translocation of NF-κB p65 to the nucleus were reduced after LPS stimulation, to a greater extent with CAPE than with DBL. Interestingly, the phosphorylation of p65 was reduced by both compounds, especially by CAPE, even when the level of IκB was not altered. Furthermore, the thiol groups of p65 were modified by CAPE, and the inhibitory effects of CAPE and DBL on the p65 phosphorylation and nitrite production were reversed by pretreatment with thiol-containing reagents. These results suggest that CAPE has strong inhibitory effects on the NF-κB activation that are associated with the modification of thiol groups and phosphorylation of p65.

Topics: Animals; Anti-Inflammatory Agents; Caffeic Acids; Cell Nucleus; Depression, Chemical; Inflammation; Interleukin-1beta; Mice; NF-kappa B; Nitric Oxide Synthase; Nitrites; Phenylethyl Alcohol; Phosphorylation; RAW 264.7 Cells; Signal Transduction