Compounds > 3-3--dihexyl-2-2--oxacarbocyanine

3-3--dihexyl-2-2--oxacarbocyanine and 7-ketocholesterol

3-3--dihexyl-2-2--oxacarbocyanine has been researched along with 7-ketocholesterol* in 1 studies

Other Studies

1 other study(ies) available for 3-3--dihexyl-2-2--oxacarbocyanine and 7-ketocholesterol

Article	Year
Analysis of the fluorescence of monodansylcadaverine-positive cytoplasmic structures during 7-ketocholesterol-induced cell death. Analytical and quantitative cytology and histology, 2004, Volume: 26, Issue:1 To analyze multilamellar cytoplasmic structures by confocal laser scanning microscopy (CLSM) combined with factor analysis of biomedical image sequences (FAMIS).. After treatment of U937 cells with 7-ketocholesterol (7-keto), cytoplasmic alterations were assessed with monodansylcadaverine (MDC). By ultraviolet excitation of a confocal laser scanning microscope (UV-CLSM), spectral sequences were performed to characterize 7-keto and MDC distribution inside cells. FAMIS was used to transform the image sequences in factor curves and images.. By UV-CLSM, 7-keto fluorescence was detected together with MDC, which revealed morphologic cytoplasmic changes in cells. The factor images obtained from confocal image sequences emphasized the view of these results. These data are in agreement with biochemical characterizations of MDC-positive structures.. The combined use of confocal microscopy and FAMIS allowed us to detect MDC-positive cytoplasmic structures in 7-keto-treated cells and to colocalize MDC and 7-keto distribution. This new method confirms the usefulness of MDC as a marker of oxysterol-induced cell death. Topics: Benzimidazoles; Cadaverine; Carbocyanines; Cell Death; Cell Nucleus; Centrifugation, Density Gradient; Cytoplasmic Structures; Enzyme Inhibitors; Flow Cytometry; Humans; Image Processing, Computer-Assisted; Ketocholesterols; Membrane Potentials; Microscopy, Confocal; Mitochondria; Spectrometry, Fluorescence; Time Factors; U937 Cells	2004

Article

Year

Analysis of the fluorescence of monodansylcadaverine-positive cytoplasmic structures during 7-ketocholesterol-induced cell death.

Analytical and quantitative cytology and histology, 2004, Volume: 26, Issue:1

To analyze multilamellar cytoplasmic structures by confocal laser scanning microscopy (CLSM) combined with factor analysis of biomedical image sequences (FAMIS).. After treatment of U937 cells with 7-ketocholesterol (7-keto), cytoplasmic alterations were assessed with monodansylcadaverine (MDC). By ultraviolet excitation of a confocal laser scanning microscope (UV-CLSM), spectral sequences were performed to characterize 7-keto and MDC distribution inside cells. FAMIS was used to transform the image sequences in factor curves and images.. By UV-CLSM, 7-keto fluorescence was detected together with MDC, which revealed morphologic cytoplasmic changes in cells. The factor images obtained from confocal image sequences emphasized the view of these results. These data are in agreement with biochemical characterizations of MDC-positive structures.. The combined use of confocal microscopy and FAMIS allowed us to detect MDC-positive cytoplasmic structures in 7-keto-treated cells and to colocalize MDC and 7-keto distribution. This new method confirms the usefulness of MDC as a marker of oxysterol-induced cell death.

Topics: Benzimidazoles; Cadaverine; Carbocyanines; Cell Death; Cell Nucleus; Centrifugation, Density Gradient; Cytoplasmic Structures; Enzyme Inhibitors; Flow Cytometry; Humans; Image Processing, Computer-Assisted; Ketocholesterols; Membrane Potentials; Microscopy, Confocal; Mitochondria; Spectrometry, Fluorescence; Time Factors; U937 Cells

2004