2-phospholactic-acid and factor-420

2-phospholactic-acid has been researched along with factor-420* in 1 studies

Other Studies

1 other study(ies) available for 2-phospholactic-acid and factor-420

ArticleYear
Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis.
    Biochemistry, 2008, Mar-04, Volume: 47, Issue:9

    Coenzyme F 420 is a hydride carrier cofactor functioning in methanogenesis. One step in the biosynthesis of coenzyme F 420 involves the coupling of 2-phospho- l-lactate (LP) to 7,8-didemethyl-8-hydroxy-5-deazaflavin, the F 420 chromophore. This condensation requires an initial activation of 2-phospho- l-lactate through a pyrophosphate linkage to GMP. Bioinformatic analysis identified an uncharacterized archaeal protein in the Methanocaldococcus jannaschii genome, MJ0887, which could be involved in this transformation. The predicted MJ0887-derived protein has domain similarity with other known nucleotidyl transferases. The MJ0887 gene was cloned and overexpressed, and the purified protein was found to catalyze the formation of lactyl-2-diphospho-5'-guanosine from LP and GTP. Kinetic constants were determined for the MJ0887-derived protein with both LP and GTP substrates and are as follows: V max = 3 micromol min (-1) mg (-1), GTP K M (app) = 56 microM, and k cat/ K M (app) = 2 x 10 (4) M (-1) s (-1) and LP K M (app) = 36 microM, and k cat/ K M (app) = 4 x 10 (4) M (-1) s (-1). The MJ0887 gene product has been designated CofC to indicate its involvement in the third step of coenzyme F 420 biosynthesis.

    Topics: Archaeal Proteins; Cloning, Molecular; Coenzymes; Fluorescence Resonance Energy Transfer; Gene Expression; Guanosine Triphosphate; Kinetics; Lactates; Methanococcales; Molecular Structure; Nucleotidyltransferases; Riboflavin; Substrate Specificity

2008