Compounds > 2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide

2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide and sapropterin

2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide has been researched along with sapropterin* in 1 studies

Other Studies

1 other study(ies) available for 2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide and sapropterin

Article	Year
Evidence for a role of nitric oxide in the mediation of antiproliferative UVA effects in keratinocytes. Pharmacological research, 2002, Volume: 45, Issue:3 Using cultured human keratinocytes, the present study investigates the role of nitric oxide (NO) in the mediation of the antiproliferative effects of ultraviolet light A (UVA). UVA treatment of cells (3-21 J cm (-2)) caused a time- and dose-dependent increase in nitrite formation in a micromolar range. This effect was accompanied by a decrease in DNA synthesis by 53.5%. Moreover, UVA treatment slightly reduced cell viability by 23.8%. Preincubation of keratinocytes with the NO scavenger 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO, 10-100 microM) or the NO synthase inhibitor N(G)-monomethyl-l-arginine (l-NMMA, 30-300 microM) significantly diminished the UVA-induced increase in nitrite. PTIO as well as l-NMMA partially protected keratinocytes from UVA-induced antiproliferative effects and increased DNA synthesis by 67 or 49% of the control. The co-application of UVA irradiation (10 J cm (-2)) and the essential cofactor of NO synthases tetrahydrobiopetrin (BH4, 500 microM) led to an overadditive increase in the release of nitrite as well as to a decrease in DNA synthesis. These results imply that NO is involved in the antiproliferative UVA effects in keratinocytes. Topics: Antioxidants; Biopterins; Cell Division; Cell Survival; Cells, Cultured; Cyclic N-Oxides; DNA; Dose-Response Relationship, Radiation; Free Radical Scavengers; Humans; Imidazoles; Keratinocytes; Nitric Oxide; Nitric Oxide Synthase; omega-N-Methylarginine; Ultraviolet Rays	2002

Article

Year

Evidence for a role of nitric oxide in the mediation of antiproliferative UVA effects in keratinocytes.

Pharmacological research, 2002, Volume: 45, Issue:3

Using cultured human keratinocytes, the present study investigates the role of nitric oxide (NO) in the mediation of the antiproliferative effects of ultraviolet light A (UVA). UVA treatment of cells (3-21 J cm (-2)) caused a time- and dose-dependent increase in nitrite formation in a micromolar range. This effect was accompanied by a decrease in DNA synthesis by 53.5%. Moreover, UVA treatment slightly reduced cell viability by 23.8%. Preincubation of keratinocytes with the NO scavenger 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO, 10-100 microM) or the NO synthase inhibitor N(G)-monomethyl-l-arginine (l-NMMA, 30-300 microM) significantly diminished the UVA-induced increase in nitrite. PTIO as well as l-NMMA partially protected keratinocytes from UVA-induced antiproliferative effects and increased DNA synthesis by 67 or 49% of the control. The co-application of UVA irradiation (10 J cm (-2)) and the essential cofactor of NO synthases tetrahydrobiopetrin (BH4, 500 microM) led to an overadditive increase in the release of nitrite as well as to a decrease in DNA synthesis. These results imply that NO is involved in the antiproliferative UVA effects in keratinocytes.

Topics: Antioxidants; Biopterins; Cell Division; Cell Survival; Cells, Cultured; Cyclic N-Oxides; DNA; Dose-Response Relationship, Radiation; Free Radical Scavengers; Humans; Imidazoles; Keratinocytes; Nitric Oxide; Nitric Oxide Synthase; omega-N-Methylarginine; Ultraviolet Rays

2002