2-nitrophenylhydrazine and stearic-acid

2-nitrophenylhydrazine has been researched along with stearic-acid* in 1 studies

Other Studies

1 other study(ies) available for 2-nitrophenylhydrazine and stearic-acid

ArticleYear
[Determination of magnesium stearate in pharmaceutical preparations using derivatization with 2-nitrophenylhydrazine and HPLC].
    Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 2005, Volume: 125, Issue:3

    In general, spectrophotometric methods (inductively coupled plasma or atomic absorption spectrophotometry) are used for the assay of magnesium stearate (Mg-St). In this study, a new rapid, selective assay method was developed for Mg-St in pharmaceutical formulations. The method was based on isocratic reverse-phase liquid chromatography using a mobile phase of acetonitrile-water (80:20, v/v) after precolumn derivatization with 2-nitrophenyl hydrazine for sensitive UV detection. Margaric acid was used as an internal standard and the substances were detectable at 230 nm or 400 nm. Using a short (2 cm) HPLC column reduced the analytical time to 5 min. Validation of the newly developed method was performed in accordance with the International Conference on Harmonization guidelines. The linearity range for Mg-St was 0.00-0.04 mg/ml (as the concentration of injected sample solution) and their correlation factor was 0.9998. The determination and detection limits for Mg-St were 6 microg and 2 microg, respectively. The proposed method was successfully applied to the determination of trace amounts of Mg-St in commercially available tablets with a high recovery percentage, good accuracy, and precision.

    Topics: Acetonitriles; Chromatography, High Pressure Liquid; Hydrazines; Pharmaceutical Preparations; Phenylhydrazines; Sensitivity and Specificity; Stearic Acids; Water

2005