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2-nitro-5-thiocyanobenzoic acid and cysteine

2-nitro-5-thiocyanobenzoic acid has been researched along with cysteine in 27 studies

Research

Studies (27)

TimeframeStudies, this research(%)All Research%
pre-199011 (40.74)18.7374
1990's7 (25.93)18.2507
2000's8 (29.63)29.6817
2010's0 (0.00)24.3611
2020's1 (3.70)2.80

Authors

AuthorsStudies
Otieno, S1
Dalton, H; Smith, DD1
Avila, J; Correas, I; López-Otín, C; Nieto, A1
Buchs, A; Carter-Su, C; May, JM1
Cetta, G; Mottes, M; Pignatti, PF; Rossi, A; Tenni, R; Valli, M1
Chapot, MP; Eshdat, Y; Strosberg, AD1
Sutherland, C; Walsh, MP1
Kato, H; Kimura, A; Nishioka, T; Oda, J; Tanaka, T1
Fukui, S; Ichikawa, M; Kakizono, T; Nihira, T; Taguchi, H; Toraya, T; Yasuda, T1
Allen, G; Gurnett, LP1
Ogilvie, JW1
Burns, RG; Islam, K1
Gracy, RW; Lu, HS1
Gaarn, A; Ramjeesingh, M; Rothstein, A1
Subramanian, AR1
Gage, DA; Watson, JT; Wu, J1
Chu, Y; Lee, EY; Reimann, EM; Schlender, KK; Wilson, SE1
Abian, J; Caminade, E; Canosa, D; Carrascal, M; Daniel, R; Le Goffic, F; Martel, A1
Chang, SI; Choi, JD; Chong, CK; Shin, HJ1
Kunugi, S; Mitani, D; Tanaka, N1
Bubis, J; Möller, C; Ortiz, JO1
Speicher, DW; Tang, HY1
Haska, CL; Hou, Z; Matherly, LH; Stapels, SE1
Belghazi, M; Cahoreau, C; Combarnous, Y; Klett, D1
Gardonio, D; Siemann, S1
Clerens, S; Deb-Choudhury, S; Dyer, JM; Koehn, H; Morton, JD; Plowman, JE1
Leeuwon, SZ; Liu, WR; Qiao, Y; Yu, G1

Other Studies

27 other study(ies) available for 2-nitro-5-thiocyanobenzoic acid and cysteine

ArticleYear
Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing.
    Biochemistry, 1978, Dec-12, Volume: 17, Issue:25

    Topics: Amino Acid Sequence; Autoanalysis; Cysteine; Dithionitrobenzoic Acid; Globins; Glycerophosphates; Hexokinase; Nickel; Nitrobenzoates; Peptide Fragments; Phosphotransferases; Saccharomyces cerevisiae; Sulfhydryl Reagents; Thiocyanates

1978
Evidence for two histidine ligands at the diiron site of methane monooxygenase.
    European journal of biochemistry, 1992, Dec-01, Volume: 210, Issue:2

    Topics: Apoenzymes; Binding Sites; Circular Dichroism; Cysteine; Diethyl Pyrocarbonate; Histidine; Iron; Kinetics; Methylococcaceae; Oxygenases; Protein Structure, Secondary; Thiocyanates

1992
Tau-related protein present in paired helical filaments has a decreased tubulin binding capacity as compared with microtubule-associated protein tau.
    Biochimica et biophysica acta, 1991, Apr-15, Volume: 1096, Issue:3

    Topics: Alzheimer Disease; Amino Acid Sequence; Brain Chemistry; Cysteine; Humans; Molecular Sequence Data; Thiocyanates; Tubulin

1991
Localization of a reactive exofacial sulfhydryl on the glucose carrier of human erythrocytes.
    Biochemistry, 1990, Nov-13, Volume: 29, Issue:45

    Topics: Binding Sites; Biological Transport; Bromosuccinimide; Cysteine; Cytochalasin B; Cytosol; Erythrocyte Membrane; Erythrocytes; Glucose; Humans; Maleimides; Monosaccharide Transport Proteins; Sulfhydryl Compounds; Thiocyanates; Trypsin

1990
Anomalous cysteine in type I collagen. Localisation by chemical cleavage of the protein using 2-nitro-5-thiocyanobenzoic acid and by mismatch analysis of cDNA heteroduplexes.
    Matrix (Stuttgart, Germany), 1990, Volume: 10, Issue:1

    Topics: Base Sequence; Collagen; Cysteine; DNA; Fibroblasts; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Osteogenesis Imperfecta; Polymerase Chain Reaction; Skin; Thiocyanates

1990
Chemical characterization of ligand binding site fragments from turkey beta-adrenergic receptor.
    FEBS letters, 1989, Mar-27, Volume: 246, Issue:1-2

    Topics: Affinity Labels; Animals; Binding Sites; Cysteine; Diazomethane; Erythrocyte Membrane; Ethylamines; Glycoside Hydrolases; Hydroxylamine; Hydroxylamines; Indicators and Reagents; Iodine Radioisotopes; Iodocyanopindolol; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; Molecular Weight; Peptide Fragments; Pindolol; Receptors, Adrenergic, beta; Serine Endopeptidases; Thiocyanates; Turkeys

1989
Phosphorylation of caldesmon prevents its interaction with smooth muscle myosin.
    The Journal of biological chemistry, 1989, Jan-05, Volume: 264, Issue:1

    Topics: Actins; Adenosine Triphosphate; Animals; Calmodulin; Calmodulin-Binding Proteins; Chickens; Chromatography, Affinity; Cysteine; Gizzard, Avian; Muscle, Smooth; Myosins; Peptide Fragments; Phosphorylation; Sulfhydryl Reagents; Thiocyanates

1989
Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis.
    The Journal of biological chemistry, 1988, Aug-25, Volume: 263, Issue:24

    Topics: Alanine; Amino Acids; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Codon; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Genes, Bacterial; Glutathione Synthase; Mutation; p-Chloromercuribenzoic Acid; Peptide Synthases; Plasmids; Sulfhydryl Compounds; Thiocyanates; Transformation, Bacterial

1988
Functional role of cysteinyl residues in tryptophanase.
    European journal of biochemistry, 1985, May-15, Volume: 149, Issue:1

    Topics: Catalysis; Chemical Phenomena; Chemistry; Cysteine; Dithiothreitol; Escherichia coli; Hydrogen-Ion Concentration; Kinetics; Lyases; Oxidation-Reduction; Specimen Handling; Thiocyanates; Tryptophanase

1985
Locations of the six disulphide bonds in a variant surface glycoprotein (VSG 117) from Trypanosoma brucei.
    The Biochemical journal, 1983, Feb-01, Volume: 209, Issue:2

    Topics: Animals; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Disulfides; Endopeptidases; Glycoproteins; Indicators and Reagents; Membrane Proteins; Pepsin A; Peptide Fragments; Serine Endopeptidases; Sulfhydryl Reagents; Thiocyanates; Trypanosoma brucei brucei; Trypsin; Variant Surface Glycoproteins, Trypanosoma

1983
Cleavage of phosphofructokinase at S-cyanylated cysteine residues.
    Biochimica et biophysica acta, 1980, Apr-25, Volume: 622, Issue:2

    Topics: Animals; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Molecular Weight; Peptide Fragments; Phosphofructokinase-1; Protein Conformation; Protein Denaturation; Rabbits; Thiocyanates

1980
Stoichiometry of microtubule-associated protein (MAP2):tubulin and the localisation of the phosphorylation and cysteine residues along the MAP2 primary sequence.
    European journal of biochemistry, 1984, Jun-15, Volume: 141, Issue:3

    Topics: Amino Acid Sequence; Animals; Binding Sites; Brain Chemistry; Chemical Phenomena; Chemistry; Chickens; Chymotrypsin; Cysteine; Electrophoresis, Polyacrylamide Gel; Microtubule-Associated Proteins; Phosphorylation; Protein Binding; Protein Kinases; Proteins; Thiocyanates; Tubulin

1984
Specific cleavage of glucosephosphate isomerases at cysteinyl residues using 2-nitro-5-thiocyanobenzoic acid: analyses of peptides eluted from polyacrylamide gels and localization of active site histidyl and lysyl residues.
    Archives of biochemistry and biophysics, 1981, Volume: 212, Issue:2

    Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Female; Glucose-6-Phosphate Isomerase; Histidine; Humans; Lysine; Muscles; Peptide Fragments; Placenta; Pregnancy; Sulfhydryl Reagents; Swine; Thiocyanates

1981
The locations of the three cysteine residues in the primary structure of the intrinsic segments of band 3 protein, and implications concerning the arrangement of band 3 protein in the bilayer.
    Biochimica et biophysica acta, 1983, Mar-23, Volume: 729, Issue:1

    Topics: Amino Acid Sequence; Anion Exchange Protein 1, Erythrocyte; Blood Proteins; Cysteine; Erythrocyte Membrane; Erythrocytes; Humans; Lipid Bilayers; Membrane Lipids; Molecular Weight; Thiocyanates

1983
Sulfhydryl groups of Escherichia coli ribosomal protein S1. Location along the polypeptide chain.
    The Journal of biological chemistry, 1980, Apr-25, Volume: 255, Issue:8

    Topics: Cysteine; Escherichia coli; Ethylmaleimide; Peptides; Ribosomal Proteins; Thiocyanates; Urea

1980
A strategy to locate cysteine residues in proteins by specific chemical cleavage followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Analytical biochemistry, 1996, Mar-15, Volume: 235, Issue:2

    Topics: Cysteine; Indicators and Reagents; Molecular Weight; Phosphines; Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Reagents; Thiocyanates

1996
Effect of activation of protein phosphatase 1 on sulfhydryl reactivity.
    Archives of biochemistry and biophysics, 1996, Oct-01, Volume: 334, Issue:1

    Topics: Animals; Binding Sites; Cattle; Cobalt; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; In Vitro Techniques; Kinetics; Manganese; Myocardium; Phosphoprotein Phosphatases; Protein Conformation; Protein Denaturation; Protein Phosphatase 1; Recombinant Proteins; Sulfhydryl Reagents; Thiocyanates

1996
Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagent.
    The Journal of biological chemistry, 1997, Oct-24, Volume: 272, Issue:43

    Topics: Binding Sites; Cysteine; Dihydroorotase; Dithionitrobenzoic Acid; Escherichia coli; Guanidine; Kinetics; Macromolecular Substances; Peptide Fragments; Spectrometry, Mass, Secondary Ion; Sulfhydryl Reagents; Thiocyanates

1997
Structural and functional role of cysteinyl residues in tobacco acetolactate synthase.
    Biochemical and biophysical research communications, 2000, May-19, Volume: 271, Issue:3

    Topics: Acetolactate Synthase; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Flavin-Adenine Dinucleotide; Herbicides; Kinetics; Mutagenesis, Site-Directed; Nicotiana; Peptide Fragments; Plants, Toxic; Protein Binding; Protein Conformation; Spectrophotometry; Thiocyanates

2000
Pressure-induced perturbation on the active site of beta-amylase monitored from the sulfhydryl reaction.
    Biochemistry, 2001, May-22, Volume: 40, Issue:20

    Topics: beta-Amylase; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Guanidine; Pressure; Protein Conformation; Protein Folding; Solanaceae; Spectrometry, Fluorescence; Sulfhydryl Reagents; Thermodynamics; Thiocyanates

2001
Chemical modification of transducin with iodoacetic acid: transducin-alpha carboxymethylated at Cys(347) allows transducin binding to Light-activated rhodopsin but prevents its release in the presence of GTP.
    Archives of biochemistry and biophysics, 2001, Nov-15, Volume: 395, Issue:2

    Topics: Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Guanine; Guanosine Diphosphate; Guanosine Triphosphate; Iodoacetic Acid; Light; Magnetic Resonance Spectroscopy; Models, Molecular; Nucleotides; Peptides; Protein Binding; Protein Conformation; Retina; Rhodopsin; Thiocyanates; Time Factors; Transducin; Trypsin

2001
Identification of alternative products and optimization of 2-nitro-5-thiocyanatobenzoic acid cyanylation and cleavage at cysteine residues.
    Analytical biochemistry, 2004, Nov-01, Volume: 334, Issue:1

    Topics: Cysteine; Nitriles; Oligopeptides; Thiocyanates

2004
Localization of a substrate binding domain of the human reduced folate carrier to transmembrane domain 11 by radioaffinity labeling and cysteine-substituted accessibility methods.
    The Journal of biological chemistry, 2005, Oct-28, Volume: 280, Issue:43

    Topics: Amino Acid Sequence; Anions; Aspartic Acid; Blotting, Western; Cell Line; Cell Membrane; Cysteine; Cytosol; Folic Acid; Glutamine; HeLa Cells; Histidine; Humans; K562 Cells; Leucovorin; Membrane Transport Proteins; Mesylates; Microscopy, Fluorescence; Molecular Sequence Data; Mutagenesis; Mutation; Peptides; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Reduced Folate Carrier Protein; Sulfhydryl Reagents; Temperature; Thiocyanates; Time Factors; Transfection; Tyrosine

2005
Nitro-thiocyanobenzoic acid (NTCB) reactivity of cysteines beta100 and beta110 in porcine luteinizing hormone: metastability and hypothetical isomerization of the two disulfide bridges of its beta-subunit seatbelt.
    Molecular and cellular endocrinology, 2006, Mar-09, Volume: 247, Issue:1-2

    Topics: Amino Acid Sequence; Animals; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Hydrogen-Ion Concentration; Luteinizing Hormone; Luteinizing Hormone, beta Subunit; Molecular Sequence Data; Peptide Fragments; Protein Denaturation; Protein Folding; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Swine; Temperature; Thiocyanates

2006
Chelator-facilitated chemical modification of IMP-1 metallo-beta-lactamase and its consequences on metal binding.
    Biochemical and biophysical research communications, 2009, Mar-27, Volume: 381, Issue:1

    Topics: beta-Lactamases; Chelating Agents; Cysteine; Dinitrobenzenes; Methods; Methyl Methanesulfonate; Phosphines; Protein Binding; Thiocyanates; Zinc

2009
Higher sequence coverage and improved confidence in the identification of cysteine-rich proteins from the wool cuticle using combined chemical and enzymatic digestion.
    Journal of proteomics, 2009, Dec-01, Volume: 73, Issue:2

    Topics: Amino Acid Sequence; Animals; Cysteine; Keratins; Peptide Fragments; Thiocyanates; Trypsin; Wool

2009
Site-Specific Conversion of Cysteine in a Protein to Dehydroalanine Using 2-Nitro-5-thiocyanatobenzoic Acid.
    Molecules (Basel, Switzerland), 2021, Apr-29, Volume: 26, Issue:9

    Topics: Alanine; Chromatography, High Pressure Liquid; Chromatography, Liquid; Cysteine; Models, Molecular; Protein Conformation; Protein Processing, Post-Translational; Proteins; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization; Thiocyanates

2021