2-hydrazino-1-methylpyridine and 2-fluoro-1-methylpyridinium

2-hydrazino-1-methylpyridine has been researched along with 2-fluoro-1-methylpyridinium* in 1 studies

Other Studies

1 other study(ies) available for 2-hydrazino-1-methylpyridine and 2-fluoro-1-methylpyridinium

Article	Year
2-hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005, Oct-25, Volume: 825, Issue:2 A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60 degrees C within 1h and the resulting derivatives of the mono-oxosteroids provided a 70-1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids, such as androstenedione and progesterone. The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram level of the androgen with a 10-mg sample. Topics: Androgens; Chromatography, Liquid; Dihydrotestosterone; Humans; Hydrazines; Ketosteroids; Male; Prostate; Prostatic Hyperplasia; Pyridines; Pyridinium Compounds; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Testosterone	2005

Article

Year

2-hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005, Oct-25, Volume: 825, Issue:2

A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60 degrees C within 1h and the resulting derivatives of the mono-oxosteroids provided a 70-1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids, such as androstenedione and progesterone. The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram level of the androgen with a 10-mg sample.

Topics: Androgens; Chromatography, Liquid; Dihydrotestosterone; Humans; Hydrazines; Ketosteroids; Male; Prostate; Prostatic Hyperplasia; Pyridines; Pyridinium Compounds; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Testosterone

2005