2-furoyl-ligrlo-amide and capsazepine

The capsaicin receptor, transient receptor potential vanilloid receptor 1 (TRPV1), colocalizes with protease-activated receptors (PARs) in the urinary bladder, but functional interaction between TRPV1 and PARs remains to be clarified. In the present study, the role of capsaicin-sensitive sensory neurons in contraction induced by activation of PAR-2 was investigated in the rat urinary bladders. The contractile responses to trypsin and the PAR-2 agonist 2-furoyl-LIGRL-NH(2) were significantly attenuated by either desensitization of sensory neurons achieved by capsaicin (10 microM) or by the TRPV1 antagonist capsazepine (30 microM). These results suggest that stimulation of capsaicin-sensitive sensory fibers contributes, at least in part, to PAR-2-mediated bladder contractions in rats.

Topics: Acetylcholine; Analgesics, Non-Narcotic; Analysis of Variance; Animals; Capsaicin; Dose-Response Relationship, Drug; Male; Muscle Contraction; Muscle, Smooth; Neurons, Afferent; Oligopeptides; Rats; Rats, Wistar; Receptor, PAR-2; Trypsin; Urinary Bladder