Compounds > 2-amino-5-6-7-trimethyl-1-8-naphthyridine

2-amino-5-6-7-trimethyl-1-8-naphthyridine and 7-8-dimethylalloxazine

2-amino-5-6-7-trimethyl-1-8-naphthyridine has been researched along with 7-8-dimethylalloxazine* in 2 studies

Other Studies

2 other study(ies) available for 2-amino-5-6-7-trimethyl-1-8-naphthyridine and 7-8-dimethylalloxazine

Article	Year
Competitive binding of abasic site-binding ligands and masking ligands to DNA duplexes for the analysis of single-base mutation. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2013, Volume: 29, Issue:1 We describe a competitive binding assay for the analysis of single-base mutation by using abasic site (AP site)-specific binding fluorescent ligands and masking ligands bound to the AP site in DNA duplexes. 2-Amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) can strongly bind to not only cytosine (C), but also thymine (T) opposite an AP site in the DNA duplexes (K(11)/10(7) M(-1): C, 15; T, 7.0). By contrast, in the presence of lumichrome (Lch) as a masking ligand, ATMND shows a clear binding selectivity for C over T (K(11)/10(7) M(-1): C, 7.0; T, 0.57), which is ascribed to the effective masking effect of Lch to the ATMND-T interaction in the AP site-containing DNA duplexes. It was shown that the competitive binding of ATMND and Lch to the AP site allowed the highly selective detection of C-related single-base mutation in DNAs amplified by polymerase chain reaction. Topics: Binding, Competitive; Cytosine; DNA; DNA Mutational Analysis; Flavins; Ligands; Models, Molecular; Naphthyridines; Point Mutation; Polymerase Chain Reaction; Spectrometry, Fluorescence; Thymine	2013
Competitive binding of small ligands to nucleobases in AP site-containing DNA duplexes. Nucleic acids symposium series (2004), 2008, Issue:52 By using lumichrome (Lch) as a masking ligand, we successfully control the binding selectivity of 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) when binding to nucleobases in AP site-containing DNA duplexes (5'-TCT GCG TCC AGX GCA ACG CAC AC-3'/3'-AGA CGC AGG TCN CGT TGC GTG TG-5', X = AP site; Spacer C3, N = C or T). In solutions buffered to pH 7.0 (I = 0.11 M, at 5 degrees C), ATMND binds to cytosine and thymine with a comparable binding affinity (K(d) / nM: C: 7.7, T: 15). By contrast, in the presence of Lch, ATMND shows a clear binding selectivity for cytosine over thymine (K(d)/nM: C: 17, T: 204). Such competitive binding events are discussed with a view towards development of ligand-based fluorescence assay for single-nucleotide polymorphisms (SNPs) typing. Topics: Binding, Competitive; Cytosine; DNA; Flavins; Ligands; Naphthyridines; Polymorphism, Single Nucleotide; Spectrometry, Fluorescence; Thymine	2008

Article

Year

Competitive binding of abasic site-binding ligands and masking ligands to DNA duplexes for the analysis of single-base mutation.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2013, Volume: 29, Issue:1

We describe a competitive binding assay for the analysis of single-base mutation by using abasic site (AP site)-specific binding fluorescent ligands and masking ligands bound to the AP site in DNA duplexes. 2-Amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) can strongly bind to not only cytosine (C), but also thymine (T) opposite an AP site in the DNA duplexes (K(11)/10(7) M(-1): C, 15; T, 7.0). By contrast, in the presence of lumichrome (Lch) as a masking ligand, ATMND shows a clear binding selectivity for C over T (K(11)/10(7) M(-1): C, 7.0; T, 0.57), which is ascribed to the effective masking effect of Lch to the ATMND-T interaction in the AP site-containing DNA duplexes. It was shown that the competitive binding of ATMND and Lch to the AP site allowed the highly selective detection of C-related single-base mutation in DNAs amplified by polymerase chain reaction.

Topics: Binding, Competitive; Cytosine; DNA; DNA Mutational Analysis; Flavins; Ligands; Models, Molecular; Naphthyridines; Point Mutation; Polymerase Chain Reaction; Spectrometry, Fluorescence; Thymine

2013

Competitive binding of small ligands to nucleobases in AP site-containing DNA duplexes.

Nucleic acids symposium series (2004), 2008, Issue:52

By using lumichrome (Lch) as a masking ligand, we successfully control the binding selectivity of 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) when binding to nucleobases in AP site-containing DNA duplexes (5'-TCT GCG TCC AGX GCA ACG CAC AC-3'/3'-AGA CGC AGG TCN CGT TGC GTG TG-5', X = AP site; Spacer C3, N = C or T). In solutions buffered to pH 7.0 (I = 0.11 M, at 5 degrees C), ATMND binds to cytosine and thymine with a comparable binding affinity (K(d) / nM: C: 7.7, T: 15). By contrast, in the presence of Lch, ATMND shows a clear binding selectivity for cytosine over thymine (K(d)/nM: C: 17, T: 204). Such competitive binding events are discussed with a view towards development of ligand-based fluorescence assay for single-nucleotide polymorphisms (SNPs) typing.

Topics: Binding, Competitive; Cytosine; DNA; Flavins; Ligands; Naphthyridines; Polymorphism, Single Nucleotide; Spectrometry, Fluorescence; Thymine

2008