2-acetyl-1-pyrroline and (pentafluorophenyl)hydrazine

The GC-SNIF technique was used to obtain the olfactograms of shrimps, and their impact odorants were identified by MS hyphenated to the GC/olfactometric system and by comprehensive two-dimensional GC hyphenated to a time-of-flight MS. Confirming these identifications by their linear retention indices required application of a new strategy to compare retention indices between both instruments and with the in-house database. The aldehydes were confirmed by using their pentafluorophenylhydrazone derivatives, and 2-ethyl-3,5-dimethyl pyrazine had to be resolved from co-eluting compounds and then identified by multidimensional GC hyphenated to an MS and a sniff port. In both shrimp products, the most important odorants were trimethylamine, 2-acetyl-1-pyrroline, and 2-ethyl-3,5-dimethyl pyrazine, together with common carbonyl compounds.

Topics: Analytic Sample Preparation Methods; Animals; Atlantic Ocean; Databases, Factual; Gas Chromatography-Mass Spectrometry; Head; Humans; Hydrazines; Indicators and Reagents; Methylamines; Molecular Structure; Odorants; Pandalidae; Polymers; Pyrazines; Pyrroles; Shellfish; Software; Volatilization