2-4-dinitrophenylhydrazine has been researched along with dityrosine* in 2 studies
2 other study(ies) available for 2-4-dinitrophenylhydrazine and dityrosine
| Article | Year |
|---|---|
Analysis of oxidative modification of proteins.
Proteins are targets of oxidative modification. This unit describes detailed procedures for the analysis of popular indices of protein oxidation including protein carbonyl formation, loss of protein thiols, and nitrotyrosine and dityrosine formation, as well as isoaspartate formation. Procedures are detailed for the analysis of protein carbonyls labeled with 2,4-dinitrophenylhydrazine, tritiated sodium borohydride, and biotin-hydrazide, followed by detection measurements that are based on the distinguishing feature of each labeling chemical. Methods are outlined for the determination of protein cysteine oxidation by quantifying the loss of free protein thiols using radiolabeled [(14)C]-iodoacetamide. Protocols are described for the measurement of protein dityrosine by gas chromatography/mass spectrometry, as are the details for the detection of protein nitrotyrosine by a competitive ELISA approach. Finally, methods are described for the quantification of protein-bound isoaspartate using protein-L-isoaspartyl methyltransferase that converts aberrant L-isoaspartyl residues in peptides and proteins to normal aspartyl residues. Topics: Biochemistry; Biotin; Borohydrides; Cysteine; Isoaspartic Acid; Isotope Labeling; Oxidation-Reduction; Phenylhydrazines; Protein Binding; Proteins; Sulfhydryl Compounds; Tyrosine | 2009 |
Analysis of oxidative modification of proteins.
Protein oxidation has been implicated in a variety of degenerative diseases as well as in the aging process. This unit describes techniques for the quantification of various protein oxidation products, including protein carbonyls, loss of protein thiol groups, dityrosine and nitrotyrosine, and isoaspartate formation. Such oxidatively modified products may also be used as biomarkers for the assessment of oxidative stress during aging and/or disease. Topics: Biochemistry; Borohydrides; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Immunoblotting; Iodoacetamide; Isoaspartic Acid; Mass Spectrometry; Oxidation-Reduction; Phenylhydrazines; Protein Carbonylation; Proteins; Reference Standards; Spectrophotometry; Sulfhydryl Compounds; Tritium; Tyrosine | 2001 |