2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid and protocatechualdehyde

A comparative analysis of ethanol extracts from peel, pulp and seed of Prunus persica var. platycarpa (Tabacchiera peach) was done. The total phenol, flavonoid and carotenoid content as well as the antioxidant properties by using different in vitro assays (DPPH, ABTS, FRAP, Fe-chelating, β-carotene bleaching test) were evaluated. Pulp extract was subjected to liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ESI-MS/MS). Gallic acid, protocatechuic acid, protocatechualdehyde, chlorogenic acid, p-coumaric acid, and ferulic acid were identified as main constituents. Pulp extract was characterized by the highest total phytonutrients content and exhibited the highest antioxidant activity in all in vitro assays (IC(50) values of 2.2 μg/mL after 60 min of incubation by using β-carotene bleaching test and 2.9 μg/mL by using Fe-chelating assay). Overall, the obtained results suggest that P. persica var. platycarpa displays a good antioxidant activity and its consumption could be promoted.

Topics: Antioxidants; Benzaldehydes; Benzothiazoles; beta Carotene; Biphenyl Compounds; Catechols; Chlorogenic Acid; Chromatography, High Pressure Liquid; Coumaric Acids; Fruit; Gallic Acid; Humans; Hydroxybenzoates; Oxidation-Reduction; Picrates; Plant Extracts; Propionates; Prunus persica; Seeds; Species Specificity; Sulfonic Acids; Tandem Mass Spectrometry