2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid and coumarin

Antioxidants have been the subject of intense research interest due to their numerous health benefits. In this work, a series of new conjugates of hydroxytyrosol and coumarin were synthesized and evaluated for their free radical scavenging, toxicity and antioxidant mechanism in vitro. The all target compounds 14a-t exhibited better radical scavenging activity than BHT, hydroxytyrosol, and coumarin in both DPPH radical and ABTS

Topics: Antioxidants; Apoptosis; Benzothiazoles; Biphenyl Compounds; Cell Line; Cell Survival; Coumarins; Dose-Response Relationship, Drug; Humans; Hydrogen Peroxide; Molecular Structure; Phenylethyl Alcohol; Picrates; Reactive Oxygen Species; Structure-Activity Relationship; Sulfonic Acids

Series of new benzyl 2H-chromenones 6a-n was synthesized by Pechmann condensation of substituted benzyl resorcinols 2a-c and 3a with various β-ketoesters such as ethyl 3-oxobutanoate, ethyl 3-oxo-3-phenylpropanoate, ethyl 4- chloro-3-oxobutanoate, ethyl 4,4,4-trifluoro-3-oxobutanoate and ethyl 2-chloro-3-oxobutanoate 5a-e in very good yields. Synthesized compounds 6a-n were screened for their α-amylase inhibitory, and ABTS.+ scavenging activities. In the present series of compounds, compound 8-benzyl-7-hydroxy-4-phenyl-2H-chromen-2-one 6c and 8-benzyl-7-hydroxy-4- methyl-2H-chromen-2-one 6a were most potent ABTS.+ radical scavenging and α-amylase inhibitor. Although compound 6,8-dibenzyl-7-hydroxy-4-(trifluoromethyl)-2H-chromen-2-one 6h displayed potent ABTS.+ free radical scavenging potential, it was found poor in inhibiting pancreatic α-amylase.

Topics: alpha-Amylases; Benzothiazoles; Coumarins; Crystallography, X-Ray; Free Radical Scavengers; Humans; Molecular Structure; Pancreas; Sulfonic Acids